S-Adenosylhomocysteine (ECMDB00939) (M2MDB000205)

General function:

Involved in S-adenosylmethionine-dependent methyltransferase activity

Specific function:

Methylation of the membrane-bound methyl-accepting chemotaxis proteins (MCP) to form gamma-glutamyl methyl ester residues in MCP

Gene Name:

cheR

Uniprot ID:

P07364

Molecular weight:

32849

Reactions

General function:

Involved in site-specific DNA-methyltransferase (adenine-specific) activity

Specific function:

The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. The EcoKI enzyme recognizes 5'-AACN(6)GTGC-3'

Gene Name:

hsdM

Uniprot ID:

P08957

Molecular weight:

59306

Reactions

General function:

Coenzyme transport and metabolism

Specific function:

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1

Gene Name:

hemX

Uniprot ID:

P09127

Molecular weight:

42963

Reactions

General function:

Involved in protein-L-isoaspartate (D-aspartate) O-methyltransferase activity

Specific function:

Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. This enzyme does not act on D-aspartyl residues

Gene Name:

pcm

Uniprot ID:

P0A7A5

Molecular weight:

23258

Reactions

General function:

Involved in RNA binding

Specific function:

Specifically methylates guanosine-37 in various tRNAs

Gene Name:

trmD

Uniprot ID:

P0A873

Molecular weight:

28422

Reactions

General function:

Involved in tRNA (guanine-N7-)-methyltransferase activity

Specific function:

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

Gene Name:

trmB

Uniprot ID:

P0A8I5

Molecular weight:

27307

Reactions

General function:

Involved in lipid biosynthetic process

Specific function:

Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge

Gene Name:

cfa

Uniprot ID:

P0A9H7

Molecular weight:

43909

Reactions

General function:

Involved in nucleic acid binding

Specific function:

Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle

Gene Name:

rsmD

Uniprot ID:

P0ADX9

Molecular weight:

21677

Reactions

General function:

Involved in methyltransferase activity

Specific function:

Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD- dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme

Gene Name:

cysG

Uniprot ID:

P0AEA8

Molecular weight:

49951

Reactions

General function:

Involved in DNA binding

Specific function:

This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands

Gene Name:

dcm

Uniprot ID:

P0AED9

Molecular weight:

53464

Reactions

General function:

Involved in site-specific DNA-methyltransferase (adenine-specific) activity

Specific function:

Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication

Gene Name:

dam

Uniprot ID:

P0AEE8

Molecular weight:

32099

Reactions

General function:

Involved in adenosylhomocysteine nucleosidase activity

Specific function:

Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively. Can also use 5'-isobutylthioadenosine, 5'-n- butylthioadenosine, S-adenosyl-D-homocysteine, decarboxylated adenosylhomocysteine, deaminated adenosylhomocysteine and S-2-aza- adenosylhomocysteine as substrates

Gene Name:

mtnN

Uniprot ID:

P0AF12

Molecular weight:

24354

Reactions

General function:

Involved in tRNA (guanosine-2'-O-)-methyltransferase activity

Specific function:

Specifically methylates guanosine-18 in various tRNAs

Gene Name:

trmH

Uniprot ID:

P0AGJ2

Molecular weight:

25343

Reactions

General function:

Involved in 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity

Specific function:

S-adenosyl-L-methionine + 3- demethylubiquinone-9 = S-adenosyl-L-homocysteine + ubiquinone-9

Gene Name:

ubiG

Uniprot ID:

P17993

Molecular weight:

26555

Reactions

General function:

Involved in tRNA (uracil-5-)-methyltransferase activity

Specific function:

Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

Gene Name:

trmA

Uniprot ID:

P23003

Molecular weight:

41967

Reactions

General function:

Involved in DNA binding

Specific function:

S-adenosyl-L-methionine + DNA adenine = S- adenosyl-L-homocysteine + DNA 6-methylaminopurine

Gene Name:

yhdJ

Uniprot ID:

P28638

Molecular weight:

33397

Reactions

General function:

Involved in methyltransferase activity

Specific function:

Specifically methylates the guanosine in position 745 of 23S rRNA

Gene Name:

rlmA

Uniprot ID:

P36999

Molecular weight:

30419

Reactions

General function:

Involved in nucleic acid binding

Specific function:

S-adenosyl-L-methionine + DNA adenine = S- adenosyl-L-homocysteine + DNA 6-methylaminopurine

Gene Name:

yfcB

Uniprot ID:

P39199

Molecular weight:

35001

Reactions

General function:

Involved in nucleic acid binding

Specific function:

Specifically methylates the guanosine in position 1207 of 16S rRNA in the 30S particle

Gene Name:

rsmC

Uniprot ID:

P39406

Molecular weight:

37624

Reactions

General function:

Involved in nucleic acid binding

Specific function:

Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA

Gene Name:

rlmG

Uniprot ID:

P42596

Molecular weight:

42331

Reactions

General function:

Involved in rRNA (adenine-N6-)-methyltransferase activity

Specific function:

Specifically methylates the adenine in position 1618 of 23S rRNA

Gene Name:

rlmF

Uniprot ID:

P75782

Molecular weight:

34226

Reactions

General function:

Involved in trans-aconitate 2-methyltransferase activity

Specific function:

Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate at high affinity and of cis- aconitate, isocitrate, and citrate at lower velocities and affinities

Gene Name:

tam

Uniprot ID:

P76145

Molecular weight:

29006

Reactions

General function:

Involved in oxidoreductase activity

Specific function:

Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm(5)s(2)U34, to form mnm(5)s(2)U34

Gene Name:

mnmC

Uniprot ID:

P77182

Molecular weight:

74434

Reactions

General function:

Involved in homocysteine S-methyltransferase activity

Specific function:

Catalyzes methyl transfer from S-methylmethionine or S- adenosylmethionine (less efficient) to homocysteine, selenohomocysteine and less efficiently selenocysteine

Gene Name:

mmuM

Uniprot ID:

Q47690

Molecular weight:

33422

Reactions

General function:

Involved in methyltransferase activity

Specific function:

Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

Gene Name:

ubiE

Uniprot ID:

P0A887

Molecular weight:

28073

Reactions

General function:

Involved in biotin biosynthetic process

Specific function:

BioC is involved in an early, but chemically unexplored, step in the conversion of pimelic acid to biotin

Gene Name:

bioC

Uniprot ID:

P12999

Molecular weight:

28276

Reactions

General function:

Not Available

Specific function:

Not Available

Gene Name:

rlmL

Uniprot ID:

P75864

Molecular weight:

Not Available

General function:

Translation, ribosomal structure and biogenesis

Specific function:

Methylates the translation termination release factor RF1/PrfA on 'Gln-235' and RF2/PrfB on 'Gln-252'

Gene Name:

hemK

Uniprot ID:

P0ACC1

Molecular weight:

30975

Reactions

General function:

Translation, ribosomal structure and biogenesis

Specific function:

Specifically methylates the ribose of guanosine 2251 in 23S rRNA

Gene Name:

rlmB

Uniprot ID:

P63177

Molecular weight:

26556

Reactions

General function:

Translation, ribosomal structure and biogenesis

Specific function:

Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA

Gene Name:

rumB

Uniprot ID:

P75817

Molecular weight:

41955

Reactions

General function:

Translation, ribosomal structure and biogenesis

Specific function:

Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

Gene Name:

rumA

Uniprot ID:

P55135

Molecular weight:

48052

Reactions

General function:

Translation, ribosomal structure and biogenesis

Specific function:

Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

Gene Name:

rlmE

Uniprot ID:

P0C0R7

Molecular weight:

23335

Reactions

General function:

Involved in protein homodimerization activity

Specific function:

Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA. Specific for fully assembled 70S ribosomes

Gene Name:

rlmH

Uniprot ID:

P0A8I8

Molecular weight:

17341

Reactions

General function:

Involved in RNA binding

Specific function:

Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA. Methylation occurs before assembly of 23S rRNA into 50S subunits

Gene Name:

rlmI

Uniprot ID:

P75876

Molecular weight:

44357

Reactions

General function:

Involved in nucleic acid binding

Specific function:

Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA. Modifies C2498 in naked 23S rRNA, but not in assembled 50S subunits or ribosomes

Gene Name:

rlmM

Uniprot ID:

P0ADR6

Molecular weight:

41905

Reactions

General function:

Involved in 4 iron, 4 sulfur cluster binding

Specific function:

Specifically methylates position 2 of adenine 2503 in 23S rRNA

Gene Name:

rlmN

Uniprot ID:

P36979

Molecular weight:

43085

Reactions

General function:

Translation, ribosomal structure and biogenesis

Specific function:

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. Has also a DNA glycosylase/AP lyase activity that removes C mispaired with oxidized T from DNA, and may play a role in protection of DNA against oxidative stress

Gene Name:

rsmA

Uniprot ID:

P06992

Molecular weight:

30420

Reactions

General function:

Defense mechanisms

Specific function:

Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA

Gene Name:

rsmB

Uniprot ID:

P36929

Molecular weight:

48347

Reactions

General function:

Involved in rRNA (uridine-N3-)-methyltransferase activity

Specific function:

Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit

Gene Name:

rsmE

Uniprot ID:

P0AGL7

Molecular weight:

26978

Reactions

General function:

Translation, ribosomal structure and biogenesis

Specific function:

Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA

Gene Name:

rsmF

Uniprot ID:

P76273

Molecular weight:

53227

Reactions

General function:

Cell wall/membrane/envelope biogenesis

Specific function:

Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

Gene Name:

rsmG

Uniprot ID:

P0A6U5

Molecular weight:

23431

Reactions

General function:

Cell wall/membrane/envelope biogenesis

Specific function:

Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA. In vitro, active on the assembled 30S subunit, but not naked 16S rRNA or 70S ribosomes

Gene Name:

rsmH

Uniprot ID:

P60390

Molecular weight:

34877

Reactions

General function:

Involved in protein binding

Specific function:

Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA. In vitro, active on the assembled 30S subunit, but not naked 16S rRNA or 70S ribosomes

Gene Name:

rsmI

Uniprot ID:

P67087

Molecular weight:

31348

Reactions

General function:

Secondary metabolites biosynthesis, transport and catabolism

Specific function:

Not Available

Gene Name:

yhiQ

Uniprot ID:

P68567

Molecular weight:

26949

Reactions

General function:

Replication, recombination and repair

Specific function:

Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA

Gene Name:

trmJ

Uniprot ID:

P0AE01

Molecular weight:

27048

Reactions

General function:

Translation, ribosomal structure and biogenesis

Specific function:

Not Available

Gene Name:

yibK

Uniprot ID:

P0AGJ7

Molecular weight:

17726

Reactions

General function:

Involved in nucleic acid binding

Specific function:

Specifically methylates the adenine in position 37 of tRNA(Val)

Gene Name:

yfiC

Uniprot ID:

P31825

Molecular weight:

27270

Reactions

General function:

tRNA methylthiolation

Specific function:

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.

Gene Name:

miaB

Uniprot ID:

P0AEI1

Molecular weight:

53662

Reactions

General function:

RNA modification

Specific function:

Catalyzes the methylthiolation of the residue Asp-89 of ribosomal protein S12.

Gene Name:

rimO

Uniprot ID:

P0AEI4

Molecular weight:

49581

Reactions