tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (P0AEI1)

Identification
Name:tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Synonyms:Not Available
Gene Name:miaB
Enzyme Class:
Biological Properties
General Function:tRNA methylthiolation
Specific Function:Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
2.0Thumb ↔ 1.0Thumb+2.0Thumb+1.0Thumb
2.0S-Adenosylmethionine ↔ 1.0S-Adenosylhomocysteine + 2.0L-Methionine + 1.05'-Deoxyadenosine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211745'-DeoxyadenosineMetaboCard
ECMDB00696L-MethionineMetaboCard
ECMDB00939S-AdenosylhomocysteineMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
GO Classification:
Function
4 iron, 4 sulfur cluster binding
cytoplasm
iron ion binding
N6-isopentenyladenosine methylthiotransferase activity
tRNA methylation
tRNA methylthiolation
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>1425
atgaccaaaaaactccatattaaaacctggggctgtcagatgaacgagtacgattcatcg
aagatggccgatctgctggatgccacccacggctatcaactgaccgacgtggcggaagaa
gcggatgtgctgctgctgaacacctgctcaatccgcgagaaggctcaggaaaaagtcttc
catcagttgggtcgctggaaactgttaaaagagaagaatccagacctgattatcggcgtc
ggtggctgcgtggcatcgcaagaaggcgagcacattcgccagcgcgcccactatgtcgat
attatttttgggccgcaaacgctgcaccgtctgccggagatgatcaactccgtgcgtggc
gaccgcagcccggttgtagatatcagcttcccggaaatcgagaagtttgaccgtctaccg
gaaccgcgcgccgaagggccgaccgcgtttgtctccatcatggaaggctgcaataaatat
tgcacctactgcgtggtgccttacacccgtggtgaagaggtaagccgtccgtccgacgat
attctgtttgagattgcccagcttgcggctcagggcgtgcgtgaagtcaacctgctcggt
cagaacgtgaacgcctggcgtggtgaaaactacgacggcaccaccggatcgtttgccgat
ctgctgcgtctggttgctgcgatcgacgggatcgatcgtattcgctttaccaccagccat
ccgatcgaattcaccgacgatatcatcgaagtgtatcgcgacacgccggagctggtgagc
ttcctgcatctgccggtacagagcggttccgatcgcattctgaacctgatgggccgtacc
catacggcgctggagtacaaagcgatcatccgtaaactgcgtgcggcgcgtccagatatt
cagatcagttctgatttcatcgttggcttccctggcgaaaccaccgaagacttcgagaaa
acgatgaagctgattgccgacgtcaatttcgacatgagctacagctttatcttctctgca
cgtccgggtacaccagccgccgatatggttgatgatgttccggaagaagagaagaagcag
cgtctgtatattctgcaagagcgcattaatcagcaagcgatggcgtggagccgccggatg
ctcggcaccacccagcgtattctggtagaaggtacatcgcgtaagagcatcatggagctt
tccgggcgtacggaaaataaccgcgtggtcaacttcgaaggcacgccggatatgatcggt
aaattcgtcgatgtagaaattaccgacgtctacccgaactctctgcgcggtaaagtggtg
cgtactgaagatgaaatgggtctgcgcgtggcagaaacaccggaatcagtgattgcccgt
acccgcaaagaaaacgaccttggcgtgggttattatcagccgtaa
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:474
Protein Molecular Weight:53662
Protein Theoretical pI:Not Available
Signaling Regions:Not Available
Transmembrane Regions:Not Available
Protein Sequence:
>tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVF
HQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRG
DRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDD
ILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSH
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDI
QISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQ
RLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMIG
KFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP
References
External Links:
ResourceLink
Uniprot ID:P0AEI1
Uniprot Name:MIAB_ECOLI
CCDB:MIAB_ECOLI
General Reference:Not Available