Ribosomal RNA large subunit methyltransferase F (P75782)

Identification
Name:Ribosomal RNA large subunit methyltransferase F
Synonyms:
  • 23S rRNA mA1618 methyltransferase
  • rRNA adenine N-6-methyltransferase
Gene Name:rlmF
Enzyme Class:
Biological Properties
General Function:Involved in rRNA (adenine-N6-)-methyltransferase activity
Specific Function:Specifically methylates the adenine in position 1618 of 23S rRNA
Cellular Location:Cytoplasm (Potential)
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0rRNA ↔ 1.0Thumb+1.0rRNA containing N6-methyladenine
1.0S-Adenosylmethionine + 1.0rRNA ↔ 1.0S-Adenosylhomocysteine + 1.0rRNA containing N6-methyladenine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
methyltransferase activity
N-methyltransferase activity
rRNA (adenine-N6-)-methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
rRNA metabolic process
rRNA processing
Gene Properties
Blattner:b0807
Gene OrientationClockwise
Centisome Percentage:18.14
Left Sequence End841555
Right Sequence End842481
Gene Sequence:
>927 bp
ATGACCATTGCACTGGAACTTCAACAGCTTAAAAAAACCTATCCAGGCGGCGTTCAGGCG
CTTCGTGGGATAGATTTGCAGGTCGAAGCGGGTGATTTTTATGCGCTTCTCGGGCCGAAC
GGGGCCGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGG
CGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGTTG
GGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTG
AATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTAT
CTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGG
ATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTC
GACGAACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAG
GATTTAAACGACAAAGGCACCACCATCATTCTCACCACACACTACCTGGAAGAAGCAGAA
ATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATG
AAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGC
CCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTT
GAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATT
CAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTT
AATGAAAAACAAGGAGATCGCGCATGA
Protein Properties
Pfam Domain Function:
Protein Residues:308
Protein Molecular Weight:34226
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ribosomal RNA large subunit methyltransferase F
MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSVDFANPLAVKALNKALL
AHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPL
IGVHEYGWRFTGSETSSQALSSAQAIISSNPGLNRAIRLRRQKESGAIFNGIIHKNEQYD
ATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGGQQQELWCEGGEVTFIKKMIEES
KGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTFMNDEQR
RRFVNRQR
References
External Links:
ResourceLink
Uniprot ID:P75782
Uniprot Name:RLMF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21238982
Ecogene ID:EG13319
Ecocyc:EG13319
ColiBase:b0807
Kegg Gene:b0807
EchoBASE ID:EB3103
CCDB:RLMF_ECOLI
BacMap:162135897
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Sergiev, P. V., Serebryakova, M. V., Bogdanov, A. A., Dontsova, O. A. (2008). "The ybiN gene of Escherichia coli encodes adenine-N6 methyltransferase specific for modification of A1618 of 23 S ribosomal RNA, a methylated residue located close to the ribosomal exit tunnel." J Mol Biol 375:291-300. Pubmed: 18021804