Identification
Name:Trans-aconitate 2-methyltransferase
Synonyms:Not Available
Gene Name:tam
Enzyme Class:
Biological Properties
General Function:Involved in trans-aconitate 2-methyltransferase activity
Specific Function:Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate at high affinity and of cis- aconitate, isocitrate, and citrate at lower velocities and affinities
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB23032(E)-3-(Methoxycarbonyl)pent-2-enedioateMetaboCard
ECMDB21211E-3-Carboxy-2-pentenedioate 6-methyl esterMetaboCard
ECMDB00939S-AdenosylhomocysteineMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
ECMDB00958trans-Aconitic acidMetaboCard
GO Classification:
Function
catalytic activity
methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
metabolic process
Gene Properties
Blattner:b1519
Gene OrientationClockwise
Centisome Percentage:34.60
Left Sequence End1605370
Right Sequence End1606128
Gene Sequence:
>759 bp
ATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCG
GTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGT
GGCCCAGGTAACAGCACCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGC
ATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTC
GTGGAAGCAGACATCCGCAACTGGCAACCGGTACAGGCACTCGATCTGATTTTTGCTAAT
GCCTCACTGCAATGGCTGCCCGACCACTACGAATTGTTTCCTCATCTGGTTTCTTTACTT
AATCCGCAGGGCGTGCTGGCAGTACAGATGCCAGATAACTGGCTGGAGCCGACCCATGTG
CTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCT
GGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGA
ACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACT
GGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGC
TACCATCAGATGCTGGAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGCA
TTTCCGCGTCTGTTTATTGTTGCCCGGCGTATGGAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:252
Protein Molecular Weight:29006
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Trans-aconitate 2-methyltransferase
MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG
IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLL
NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR
TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLA
FPRLFIVARRME
References
External Links:
ResourceLink
Uniprot ID:P76145
Uniprot Name:TAM_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675003
Ecogene ID:EG13812
Ecocyc:EG13812
ColiBase:b1519
Kegg Gene:b1519
EchoBASE ID:EB3573
CCDB:TAM_ECOLI
BacMap:16129478
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cai, H., Clarke, S. (1999). "A novel methyltransferase catalyzes the methyl esterification of trans-aconitate in Escherichia coli." J Biol Chem 274:13470-13479. Pubmed: 10224113
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553