DNA adenine methylase (P0AEE8)

Identification
Name:DNA adenine methylase
Synonyms:
  • DNA adenine methyltransferase
  • Deoxyadenosyl-methyltransferase
  • M.EcoDam
Gene Name:dam
Enzyme Class:
Biological Properties
General Function:Involved in site-specific DNA-methyltransferase (adenine-specific) activity
Specific Function:Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0DNA adenine ↔ 1.0Thumb+1.0DNA 6-methylaminopurine
1.0S-Adenosylmethionine + 1.0DNA adenine ↔ 1.0S-Adenosylhomocysteine + 1.0DNA 6-methylaminopurine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
GO Classification:
Function
binding
catalytic activity
methyltransferase activity
nucleic acid binding
S-adenosylmethionine-dependent methyltransferase activity
site-specific DNA-methyltransferase (adenine-specific) activity
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular macromolecule metabolic process
cellular metabolic process
DNA alkylation
DNA metabolic process
DNA methylation
DNA modification
macromolecule metabolic process
metabolic process
methylation
one-carbon metabolic process
Gene Properties
Blattner:b3387
Gene OrientationCounterclockwise
Centisome Percentage:75.72
Left Sequence End3513099
Right Sequence End3513935
Gene Sequence:
>837 bp
ATGGAAATGCTCGAAGAGCACCGCTGTTTTGAAGGCTGGCAGCAACGCTGGCGACACGAC
TCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCAC
ACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACC
ACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTACTGGTGATGCCAGAC
ACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCA
GGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTAT
CTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCC
ATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGT
AAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGA
ATCAAAGCGTTTAGCAGCTATTTAGGTGAGGACAAAAATGCATGGCTGGAATGGGACAGT
TGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATCCCGACGCTTATCGATCAGGGC
GATAATGATCAGTTTCTTGCCGACCAGTTGCAACCTGCGGTACTGGCAGAAGCCGCGCGC
CAGAAAGCGTGGCCGATGACGCTGCGTATTCAGCCGGGATATGATCACAGTTACTACTTC
ATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:278
Protein Molecular Weight:32099
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>DNA adenine methylase
MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLI
SLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQDPFRRAVLFLYLNRYGYN
GLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFFYCESYADSMARADDASVVYC
DPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVERHIPVLISNHDTMLTREWYQRA
KLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPAKK
References
External Links:
ResourceLink
Uniprot ID:P0AEE8
Uniprot Name:DMA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675268
Ecogene ID:EG10204
Ecocyc:EG10204
ColiBase:b3387
Kegg Gene:b3387
EchoBASE ID:EB0200
CCDB:DMA_ECOLI
BacMap:16131265
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Brooks, J. E., Blumenthal, R. M., Gingeras, T. R. (1983). "The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene." Nucleic Acids Res 11:837-851. Pubmed: 6300769
  • Guyot, J. B., Grassi, J., Hahn, U., Guschlbauer, W. (1993). "The role of the preserved sequences of Dam methylase." Nucleic Acids Res 21:3183-3190. Pubmed: 8341592
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jonczyk, P., Hines, R., Smith, D. W. (1989). "The Escherichia coli dam gene is expressed as a distal gene of a new operon." Mol Gen Genet 217:85-96. Pubmed: 2549371
  • Lyngstadaas, A., Lobner-Olesen, A., Boye, E. (1995). "Characterization of three genes in the dam-containing operon of Escherichia coli." Mol Gen Genet 247:546-554. Pubmed: 7603433