Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (P0A887)

Identification
Name:Ubiquinone/menaquinone biosynthesis methyltransferase ubiE
Synonyms:Not Available
Gene Name:ubiE
Enzyme Class:
Biological Properties
General Function:Involved in methyltransferase activity
Specific Function:Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)
Cellular Location:Not Available
SMPDB Pathways:
  • Menaquinol biosythesis PW001897
  • Secondary Metabolites: Ubiquinol biosynthesis PW000981
  • Secondary Metabolites: Ubiquinol biosynthesis 2 PW002036
KEGG Pathways:
  • Metabolic pathways eco01100
  • Ubiquinone and other terpenoid-quinone biosynthesis ec00130
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Menaquinone+1.0Thumb
1.02-Demethylmenaquinone 8 + 1.0S-Adenosylmethionine ↔ 1.0Menaquinone + 1.0S-Adenosylhomocysteine
ReactionCard
1.02-Phytyl-1,4-naphthoquinone+1.0Thumb ↔ 1.0Phylloquinone+1.0Thumb
1.02-Phytyl-1,4-naphthoquinone + 1.0S-Adenosylmethionine ↔ 1.0Phylloquinone + 1.0S-Adenosylhomocysteine
ReactionCard
1.02-Polyprenyl-6-methoxy-1,4-benzoquinone+1.0Thumb ↔ 1.02-Polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone+1.0Thumb
1.02-Polyprenyl-6-methoxy-1,4-benzoquinone + 1.0S-Adenosylmethionine ↔ 1.02-Polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone + 1.0S-Adenosylhomocysteine
ReactionCard
1.0Demethylmenaquinol+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Demethylmenaquinol + 1.0S-Adenosylmethionine + 1.0Demethylmenaquinol ↔ 1.0Menaquinol 6 + 1.0S-Adenosylhomocysteine
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0S-adenosyl-L-methionine → 1.0Thumb+1.0Thumb+1.0Thumb
1.02-Octaprenyl-6-methoxy-1,4-benzoquinol + 1.0S-adenosyl-L-methionine → 1.0S-Adenosylhomocysteine + 1.0Hydrogen ion + 1.06-Methoxy-3-methyl-2-all-trans-octaprenyl-1,4-benzoquinol
ReactionCard
1.0Thumb+1.0S-adenosyl-L-methionine → 1.0Thumb+1.0Thumb+1.0Thumb
1.02-Demethylmenaquinol 8 + 1.0S-adenosyl-L-methionine → 1.0Hydrogen ion + 1.0S-Adenosylhomocysteine + 1.0Menaquinol 8
ReactionCard
1.0Thumb+1.0S-adenosyl-L-methionine → 1.02-Octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol +1.0Thumb+1.0Thumb
1.02-Octaprenyl-6-methoxy-1,4-benzoquinol + 1.0S-adenosyl-L-methionine → 1.02-Octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol + 1.0S-Adenosylhomocysteine + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.02-Demethylmenaquinol 8 + 1.0S-Adenosylmethionine → 1.0S-Adenosylhomocysteine + 1.0Hydrogen ion + 1.0Menaquinol 8
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.02-Octaprenyl-6-methoxy-1,4-benzoquinol + 1.0S-Adenosylmethionine → 1.02-Octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol + 1.0S-Adenosylhomocysteine + 1.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211542-Demethylmenaquinol 8MetaboCard
ECMDB211552-Demethylmenaquinone 8MetaboCard
ECMDB211572-Octaprenyl-3-methyl-6-methoxy-1,4-benzoquinolMetaboCard
ECMDB211582-Octaprenyl-6-methoxy-1,4-benzoquinolMetaboCard
ECMDB234366-Methoxy-3-methyl-2-all-trans-octaprenyl-1,4-benzoquinolMetaboCard
ECMDB23820DemethylmenaquinolMetaboCard
ECMDB21521Demethylmenaquinone-7MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21551Menaquinol 6MetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB04102MenaquinoneMetaboCard
ECMDB00939S-AdenosylhomocysteineMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
GO Classification:
Function
catalytic activity
methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Gene Properties
Blattner:b3833
Gene OrientationClockwise
Centisome Percentage:86.58
Left Sequence End4016878
Right Sequence End4017633
Gene Sequence:
>756 bp
ATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT
GAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAG
CGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGG
CGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCA
CGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCG
ACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCC
ACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACG
CTGGTGCAGGGATCGTTACCCGAACTGCATCAGGCGTGGCAGGCGGTGGACGAGCGTCCG
CATGCTAATCGCTTTTTACCGCCAGATGAAATCGAACAGTCGCTGAACGGCGTGCATTAT
CAACATCATATTCAGCCCATCACGCTGTGGTTTGATGATGCGCTCAGTGCCATGCGTTCG
CTGAAAGGCATCGGTGCCACGCATCTTCATGAAGGGCGCGACCCGCGAATATTAACGCGT
TCGCAGTTGCAGCGATTGCAACTGGCCTGGCCGCAACAGCAGGGGCGATATCCTCTGACG
TATCATCTTTTTTTGGGAGTGATTGCTCGTGAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:251
Protein Molecular Weight:28073
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ubiquinone/menaquinone biosynthesis methyltransferase ubiE
MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMSFGIHRLWKRFTIDCSG
VRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLKMGREKLRNIGVIGNVEYV
QANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVLKPGGRLLVLEFSKPIIEPLS
KAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQDTLKAMMQDAGFESVDYYNLTA
GVVALHRGYKF
References
External Links:
ResourceLink
Uniprot ID:P0A887
Uniprot Name:UBIE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674760
Ecogene ID:EG11473
Ecocyc:EG11473
ColiBase:b3833
Kegg Gene:b3833
EchoBASE ID:EB1441
CCDB:UBIE_ECOLI
BacMap:49176426
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lee, P. T., Hsu, A. Y., Ha, H. T., Clarke, C. F. (1997). "A C-methyltransferase involved in both ubiquinone and menaquinone biosynthesis: isolation and identification of the Escherichia coli ubiE gene." J Bacteriol 179:1748-1754. Pubmed: 9045837
  • Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293