ECMDB

Identification

Name:

3-demethylubiquinone-9 3-methyltransferase

Synonyms:

3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase
DHHB methyltransferase

Gene Name:

ubiG

Enzyme Class:

Biological Properties

General Function:

Involved in 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity

Specific Function:

S-adenosyl-L-methionine + 3- demethylubiquinone-9 = S-adenosyl-L-homocysteine + ubiquinone-9

Cellular Location:

Not Available

SMPDB Pathways:

Secondary Metabolites: Ubiquinol biosynthesis PW000981
Secondary Metabolites: Ubiquinol biosynthesis 2 PW002036

KEGG Pathways:

Metabolic pathways eco01100
Ubiquinone and other terpenoid-quinone biosynthesis ec00130

KEGG Reactions:

1.0

→

1.0

1.02-Octaprenyl-6-hydroxyphenol + 1.0S-Adenosylmethionine → 1.02-Octaprenyl-6-methoxyphenol + 1.0S-Adenosylhomocysteine + 1.0Hydrogen ion
ReactionCard

1.0

↔

1.0

1.02-Octaprenyl-6-hydroxyphenol + 1.0S-Adenosylmethionine ↔ 1.02-Octaprenyl-6-methoxyphenol + 1.0S-Adenosylhomocysteine
ReactionCard

1.02-Polyprenyl-6-hydroxyphenol

1.0

↔

1.02-Polyprenyl-6-methoxyphenol

1.0

1.02-Polyprenyl-6-hydroxyphenol + 1.0S-Adenosylmethionine ↔ 1.02-Polyprenyl-6-methoxyphenol + 1.0S-Adenosylhomocysteine
ReactionCard

1.02-Polyprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone

1.0

↔

1.0

1.02-Polyprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone + 1.0S-Adenosylmethionine ↔ 1.0Ubiquinone-1 + 1.0S-Adenosylhomocysteine
ReactionCard

SMPDB Reactions:

1.02-Octaprenyl-6-hydroxyphenol

1.0S-adenosyl-L-methionine

1.0

→

1.0

1.02-Octaprenyl-6-hydroxyphenol + 1.0S-adenosyl-L-methionine + 1.02-Octaprenyl-6-hydroxyphenol → 1.0Hydrogen ion + 1.0S-Adenosylhomocysteine + 1.02-methoxy-6-(all-trans-octaprenyl)phenol
ReactionCard

1.0

1.0S-adenosyl-L-methionine

→

1.0

1.0Ubiquinol 8

1.0

1.03-demethylubiquinol-8 + 1.0S-adenosyl-L-methionine → 1.0Hydrogen ion + 1.0S-Adenosylhomocysteine + 1.0Ubiquinol 8 + 1.0Ubiquinol-8
ReactionCard

1.02-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone

1.0S-adenosyl-L-methionine

→

1.0

1.02-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone + 1.0S-adenosyl-L-methionine → 1.0S-Adenosylhomocysteine + 1.0Ubiquinol-8 + 1.0Hydrogen ion
ReactionCard

EcoCyc Reactions:

1.0

→

1.0

1.02-Octaprenyl-6-hydroxyphenol + 1.0S-Adenosylmethionine → 1.02-Octaprenyl-6-methoxyphenol + 1.0S-Adenosylhomocysteine + 1.0Hydrogen ion
ReactionCard

Complex Reactions:

1.0

→

1.0

1.02-Octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinol + 1.0S-Adenosylmethionine → 1.0S-Adenosylhomocysteine + 1.0Hydrogen ion + 1.0Ubiquinol-8
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB23437	2-methoxy-6-(all-trans-octaprenyl)phenol	MetaboCard
ECMDB21156	2-Octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinol	MetaboCard
ECMDB04117	2-Octaprenyl-6-hydroxyphenol	MetaboCard
ECMDB04119	2-Octaprenyl-6-methoxyphenol	MetaboCard
ECMDB24259	3-demethylubiquinol-8	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00939	S-Adenosylhomocysteine	MetaboCard
ECMDB01185	S-Adenosylmethionine	MetaboCard
ECMDB21579	Ubiquinol-7	MetaboCard
ECMDB01060	Ubiquinol-8	MetaboCard
ECMDB21580	Ubiquinol-9	MetaboCard
ECMDB21438	Ubiquinone-1	MetaboCard
ECMDB21581	Ubiquinone-2	MetaboCard
ECMDB20620	Ubiquinone-6	MetaboCard
ECMDB21584	Ubiquinone-7	MetaboCard
ECMDB21296	Ubiquinone-8	MetaboCard
ECMDB21583	Ubiquinone-9	MetaboCard

GO Classification:

Function
2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity
C-methyltransferase activity
catalytic activity
methyltransferase activity
quinone cofactor methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular metabolic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
oxidoreduction coenzyme metabolic process
ubiquinone biosynthetic process
ubiquinone metabolic process

Gene Properties

Blattner:

b2232

Gene Orientation

Clockwise

Centisome Percentage:

50.38

Left Sequence End

2337589

Right Sequence End

2338311

Gene Sequence:

>723 bp
ATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCC
TGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATG
TATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGC
GCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGAC
CCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTG
CCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATTGATTGATGAT
GGCTGGCTGGTCATTAGTGGCTTCAATCCCATCAGTTTTATGGGATTACGCAAACTTGTG
CCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTG
GACTGGCTCTCTTTGTTGAATTTTGAAGTGCTACACGCCAGCCGTTTCCACGTTCTCCCG
TGGAACAAACACGGAGGAAAACTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAA
CTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAAA
AACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCT
TAA

Protein Properties

Pfam Domain Function:

Methyltransf_11 (PF08241 )

Protein Residues:

240

Protein Molecular Weight:

26555

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>3-demethylubiquinone-9 3-methyltransferase
MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLRLGYIAERAGGLFGKKV
LDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESGIQVDYVQETVEEHAAKHA
GQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTLNRNGKSWLMAVVGAEYILRM
VPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNPITNTFKLGPGVDVNYMLHTQNK

References

External Links:

Resource	Link
Uniprot ID:	P17993
Uniprot Name:	UBIG_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	4902950
Ecogene ID:	EG11143
Ecocyc:	EG11143
ColiBase:	b2232
Kegg Gene:	b2232
EchoBASE ID:	EB1133
CCDB:	UBIG_ECOLI
BacMap:	16130167

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Hussain, K., Elliott, E. J., Salmond, G. P. (1987). "The parD- mutant of Escherichia coli also carries a gyrAam mutation. The complete sequence of gyrA." Mol Microbiol 1:259-273. Pubmed: 2834621
Wu, G., Williams, H. D., Zamanian, M., Gibson, F., Poole, R. K. (1992). "Isolation and characterization of Escherichia coli mutants affected in aerobic respiration: the cloning and nucleotide sequence of ubiG. Identification of an S-adenosylmethionine-binding motif in protein, RNA, and small-molecule methyltransferases." J Gen Microbiol 138:2101-2112. Pubmed: 1479344
Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837

3-demethylubiquinone-9 3-methyltransferase (P17993)