tRNA (guanine-N(7)-)-methyltransferase (P0A8I5)

Identification
Name:tRNA (guanine-N(7)-)-methyltransferase
Synonyms:
  • tRNA(m7G46)-methyltransferase
Gene Name:trmB
Enzyme Class:
Biological Properties
General Function:Involved in tRNA (guanine-N7-)-methyltransferase activity
Specific Function:Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
GO Classification:
Function
catalytic activity
methyltransferase activity
RNA methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
tRNA (guanine) methyltransferase activity
tRNA (guanine-N7-)-methyltransferase activity
tRNA methyltransferase activity
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
tRNA metabolic process
tRNA modification
tRNA processing
Gene Properties
Blattner:b2960
Gene OrientationCounterclockwise
Centisome Percentage:66.82
Left Sequence End3100155
Right Sequence End3100874
Gene Sequence:
>720 bp
GTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGA
GACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGA
TGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAA
AATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGC
ACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAATGGGCATCACGT
GCCGCAAAGGTTGCCGCGCGTATGCAGGATCTCACCTCTTTTATTGTTAATAAATTAGGG
GTAGTCGATGTAGGTGCCAGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTG
GCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTG
GAGCTGTTGACCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTC
AAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTC
CGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTA
CAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCCGCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:239
Protein Molecular Weight:27307
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>tRNA (guanine-N(7)-)-methyltransferase
MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPVMGVEFSEDMLDFPALF
GREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGACLASAHEEGLSNLRVMCH
DAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVPFAELVKSKLQLGGVFHMATD
WEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTKFEQRGHRLGHGVWDLMFERVK
References
External Links:
ResourceLink
Uniprot ID:P0A8I5
Uniprot Name:TRMB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674450
Ecogene ID:EG11779
Ecocyc:EG11779
ColiBase:b2960
Kegg Gene:b2960
EchoBASE ID:EB1727
CCDB:TRMB_ECOLI
BacMap:16130861
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • De Bie, L. G., Roovers, M., Oudjama, Y., Wattiez, R., Tricot, C., Stalon, V., Droogmans, L., Bujnicki, J. M. (2003). "The yggH gene of Escherichia coli encodes a tRNA (m7G46) methyltransferase." J Bacteriol 185:3238-3243. Pubmed: 12730187
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Purta, E., van Vliet, F., Tricot, C., De Bie, L. G., Feder, M., Skowronek, K., Droogmans, L., Bujnicki, J. M. (2005). "Sequence-structure-function relationships of a tRNA (m7G46) methyltransferase studied by homology modeling and site-directed mutagenesis." Proteins 59:482-488. Pubmed: 15789416
  • Tsai-Wu, J. J., Radicella, J. P., Lu, A. L. (1991). "Nucleotide sequence of the Escherichia coli micA gene required for A/G-specific mismatch repair: identity of micA and mutY." J Bacteriol 173:1902-1910. Pubmed: 2001994