Protein-L-isoaspartate O-methyltransferase (P0A7A5)

Identification
Name:Protein-L-isoaspartate O-methyltransferase
Synonyms:
  • L-isoaspartyl protein carboxyl methyltransferase
  • Protein L-isoaspartyl methyltransferase
  • Protein-beta-aspartate methyltransferase
  • PIMT
Gene Name:pcm
Enzyme Class:
Biological Properties
General Function:Involved in protein-L-isoaspartate (D-aspartate) O-methyltransferase activity
Specific Function:Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. This enzyme does not act on D-aspartyl residues
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Protein L-isoaspartate ↔ 1.0Thumb+1.0Protein L-isoaspartate methyl ester
1.0S-Adenosylmethionine + 1.0Protein L-isoaspartate ↔ 1.0S-Adenosylhomocysteine + 1.0Protein L-isoaspartate methyl ester
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0a [protein]-L-β-isoaspartate → 1.0Thumb+1.0a protein L-β-isoaspartate α-methyl ester+1.0Thumb
1.0S-Adenosylmethionine + 1.0a [protein]-L-β-isoaspartate → 1.0S-Adenosylhomocysteine + 1.0a protein L-β-isoaspartate α-methyl ester + 1.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB00939S-AdenosylhomocysteineMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
GO Classification:
Function
catalytic activity
methyltransferase activity
protein-L-isoaspartate (D-aspartate) O-methyltransferase activity
S-adenosylmethionine-dependent methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
macromolecule metabolic process
macromolecule modification
metabolic process
protein modification process
Gene Properties
Blattner:b2743
Gene OrientationCounterclockwise
Centisome Percentage:61.79
Left Sequence End2866915
Right Sequence End2867541
Gene Sequence:
>627 bp
ATGACATACGATAGTGAATTCGGATCACATGTATCCCTATATCGGGATAGAATCAAACAG
GTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCAT
GACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGT
GCTATATCTCGCTTTACCAGTGCGTTAAAAGCAAAGCTTAAACATGATAAGCATATTAAA
ACTCAACGGAAAGACTGGCCTGATAGTCGACATTCCACTTTACGTTACGCATGGGTCAGA
GAATATACCAAAAATAGAAAGCGGCATTACCATTTGATACTGTGTTTCAATCAGGATGCT
TATTATCATTTAGGTGATTACGACTTAAACCGTAACACGTTACGTACAATGATAACGACA
GCTTGGTACAGTGCACTTGGCATCCCTATAGATAGCTCGGGGAAGTTAGTTAATTACCCG
CCAAATGGCAAATACCTTCTCAATCGTAAAAGGGACAACTTTGAGCAGACTTATAGCGAT
TTGATGAATAGGGTGGATTACATGACCAAAGTAAGGACTAAAATAGTCGGTGACGGAGAC
CGTAATTTCGGCTGCAGTCGCGGGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:208
Protein Molecular Weight:23258
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Protein-L-isoaspartate O-methyltransferase
MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKAWDNIALPIGQGQTISQ
PYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCSVERIKGLQWQARRRLKNL
DLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMTQLDEGGILVLPVGEEHQYLK
RVRRRGGEFIIDTVEAVRFVPLVKGELA
References
External Links:
ResourceLink
Uniprot ID:P0A7A5
Uniprot Name:PIMT_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675489
Ecogene ID:EG10689
Ecocyc:EG10689
ColiBase:b2743
Kegg Gene:b2743
EchoBASE ID:EB0683
CCDB:PIMT_ECOLI
BacMap:16130650
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Fu, J. C., Ding, L., Clarke, S. (1991). "Purification, gene cloning, and sequence analysis of an L-isoaspartyl protein carboxyl methyltransferase from Escherichia coli." J Biol Chem 266:14562-14572. Pubmed: 1860862
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ichikawa, J. K., Li, C., Fu, J., Clarke, S. (1994). "A gene at 59 minutes on the Escherichia coli chromosome encodes a lipoprotein with unusual amino acid repeat sequences." J Bacteriol 176:1630-1638. Pubmed: 8132457
  • Li, C., Ichikawa, J. K., Ravetto, J. J., Kuo, H. C., Fu, J. C., Clarke, S. (1994). "A new gene involved in stationary-phase survival located at 59 minutes on the Escherichia coli chromosome." J Bacteriol 176:6015-6022. Pubmed: 7928962
  • Takayanagi, Y., Tanaka, K., Takahashi, H. (1994). "Structure of the 5' upstream region and the regulation of the rpoS gene of Escherichia coli." Mol Gen Genet 243:525-531. Pubmed: 8208244