Protein methyltransferase hemK (P0ACC1)

Identification
Name:Protein methyltransferase hemK
Synonyms:
  • M.EcoKHemKP
  • Protein-(glutamine-N(5)) MTase hemK
  • Protein-glutamine N-methyltransferase hemK
Gene Name:hemK
Enzyme Class:
Biological Properties
General Function:Translation, ribosomal structure and biogenesis
Specific Function:Methylates the translation termination release factor RF1/PrfA on 'Gln-235' and RF2/PrfB on 'Gln-252'
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
GO Classification:
Function
binding
catalytic activity
methyltransferase activity
nucleic acid binding
protein methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular metabolic process
macromolecule metabolic process
macromolecule modification
metabolic process
methylation
one-carbon metabolic process
post-translational protein modification
protein amino acid alkylation
protein amino acid methylation
protein modification process
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>834 bp
ATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCG
CGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTC
GCCTTTGGTGAAACGCAGCTGACTGACGAACAATGTCAGCAACTTGATGCGCTACTGACA
CGTCGTCGCGATGGTGAACCCATTGCTCATTTAACCGGGGTGCGAGAATTCTGGTCGTTG
CCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAG
CAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACC
GGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGAT
CGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAAT
ATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATT
GTCAGCAATCCGCCGTATATTGACGAGCAGGACCCTCATCTTCAACAAGGCGATGTCCGC
TTTGAGCCGCTCACTGCGCTGGTTGCGGCAGACAGTGGAATGGCAGACATCGTGCATATC
ATCGAACAGTCGCGTAACGCGCTGGTATCCGGCGGCTTTCTGCTTCTGGAACATGGCTGG
CAGCAGGGCGAAGCGGTGCGACAAGCATTTATCCTCGCGGGGTATCATGACGTCGAAACC
TGCCGTGACTATGGTGATAACGAGCGCGTAACGCTCGGCCGCTATTATCAATGA
Protein Properties
Pfam Domain Function:
Protein Residues:277
Protein Molecular Weight:30975
Protein Theoretical pI:5
PDB File:1T43
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Protein methyltransferase hemK
MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGETQLTDEQCQQLDALLT
RRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQALARLPEQPCRILDLGTGT
GAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKNIHILQSDWFSALAGQQFAMI
VSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIVHIIEQSRNALVSGGFLLLEHGW
QQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYYQ
References
External Links:
ResourceLink
Uniprot ID:P0ACC1
Uniprot Name:HEMK_ECOLI
GenBank Gene ID:AB078778
Genebank Protein ID:18376673
PDB ID:1T43
CCDB:HEMK_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Graille, M., Heurgue-Hamard, V., Champ, S., Mora, L., Scrima, N., Ulryck, N., van Tilbeurgh, H., Buckingham, R. H. (2005). "Molecular basis for bacterial class I release factor methylation by PrmC." Mol Cell 20:917-927. Pubmed: 16364916
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Nakahigashi, K., Kubo, N., Narita, S., Shimaoka, T., Goto, S., Oshima, T., Mori, H., Maeda, M., Wada, C., Inokuchi, H. (2002). "HemK, a class of protein methyl transferase with similarity to DNA methyl transferases, methylates polypeptide chain release factors, and hemK knockout induces defects in translational termination." Proc Natl Acad Sci U S A 99:1473-1478. Pubmed: 11805295
  • Nakayashiki, T., Nishimura, K., Inokuchi, H. (1995). "Cloning and sequencing of a previously unidentified gene that is involved in the biosynthesis of heme in Escherichia coli." Gene 153:67-70. Pubmed: 7883187
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Strohmaier, H., Remler, P., Renner, W., Hogenauer, G. (1995). "Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12." J Bacteriol 177:4488-4500. Pubmed: 7543480
  • Yang, Z., Shipman, L., Zhang, M., Anton, B. P., Roberts, R. J., Cheng, X. (2004). "Structural characterization and comparative phylogenetic analysis of Escherichia coli HemK, a protein (N5)-glutamine methyltransferase." J Mol Biol 340:695-706. Pubmed: 15223314