Ribosomal RNA large subunit methyltransferase G (P42596)

Identification
Name:Ribosomal RNA large subunit methyltransferase G
Synonyms:
  • 23S rRNA m2G1835 methyltransferase
  • rRNA (guanine-N(2)-)-methyltransferase rlmG
Gene Name:rlmG
Enzyme Class:
Biological Properties
General Function:Involved in nucleic acid binding
Specific Function:Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA
Cellular Location:Cytoplasm (Potential)
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0rRNA ↔ 1.0Thumb+1.0rRNA containing N2-methylguanine
1.0S-Adenosylmethionine + 1.0rRNA ↔ 1.0S-Adenosylhomocysteine + 1.0rRNA containing N2-methylguanine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
binding
catalytic activity
methyltransferase activity
N-methyltransferase activity
nucleic acid binding
rRNA (guanine-N2-)-methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular macromolecule metabolic process
cellular metabolic process
macromolecule metabolic process
metabolic process
methylation
ncRNA metabolic process
one-carbon metabolic process
RNA metabolic process
rRNA metabolic process
rRNA methylation
rRNA modification
rRNA processing
Gene Properties
Blattner:b3084
Gene OrientationCounterclockwise
Centisome Percentage:69.68
Left Sequence End3232761
Right Sequence End3233897
Gene Sequence:
>1137 bp
ATGTTTGATTATGAAACATTGCGCTTCATCTGGTGGCTGCTGATTGGCGTGATCCTGGTG
GTCTTTATGATCTCCGACGGATTTGACATGGGGATCGGCTGTCTGCTGCCGCTGGTGGCG
CGTAATGATGATGAACGCCGGATAGTGATAAACAGCGTTGGTGCACACTGGGAAGGCAAC
CAGGTCTGGTTGATCCTCGCTGGTGGGGCATTATTTGCCGCCTGGCCCAGAGTGTATGCA
GCGGCGTTTTCCGGCTTTTATGTGGCGATGATCCTGGTGCTGTGCTCACTGTTCTTCCGC
CCGCTGGCCTTTGATTATCGCGGAAAAATCGCCGATGCACGCTGGCGTAAAATGTGGGAC
GCCGGTCTGGTCATCGGCAGTCTGGTGCCGCCGGTAGTCTTCGGTATCGCCTTCGGCAAC
TTGTTGCTCGGCGTGCCGTTTGCCTTCACACCGCAATTACGCGTGGAGTATCTCGGCAGC
TTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTG
ATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGTTGGTGTGATTCATCTGCGTTCA
CAGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTAC
TGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCC
AATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAG
TCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATG
GCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGC
GTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCG
ATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTG
GTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGCTACTACAAA
ATGTGGGGGCGCATGACAACAGAAACTCTCCGCCGTAACGAAAACGAGTTGTACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:378
Protein Molecular Weight:42331
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ribosomal RNA large subunit methyltransferase G
MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEIRGPVLILNDAFGALSC
ALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLAL
LEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLGPTTTTLAWKKARLINCTFNE
PQLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNG
VIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNMPEALDRCEFMINNALSGVEPFRFN
AVLCNPPFHQQHALTDNVAWEMFHHARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTI
ATNNKFVVLKAVKLGRRR
References
External Links:
ResourceLink
Uniprot ID:P42596
Uniprot Name:RLMG_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651480
Ecogene ID:EG12726
Ecocyc:EG12726
ColiBase:b3084
Kegg Gene:b3084
EchoBASE ID:EB2585
CCDB:RLMG_ECOLI
BacMap:90111535
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Sergiev, P. V., Lesnyak, D. V., Bogdanov, A. A., Dontsova, O. A. (2006). "Identification of Escherichia coli m2G methyltransferases: II. The ygjO gene encodes a methyltransferase specific for G1835 of the 23 S rRNA." J Mol Biol 364:26-31. Pubmed: 17010380