23S rRNA (guanosine-2'-O-)-methyltransferase rlmB (P63177)

Identification
Name:23S rRNA (guanosine-2'-O-)-methyltransferase rlmB
Synonyms:
  • 23S rRNA Gm2251 2'-O-methyltransferase
Gene Name:rlmB
Enzyme Class:
Biological Properties
General Function:Translation, ribosomal structure and biogenesis
Specific Function:Specifically methylates the ribose of guanosine 2251 in 23S rRNA
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
GO Classification:
Function
binding
catalytic activity
methyltransferase activity
nucleic acid binding
RNA binding
RNA methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
RNA metabolic process
RNA processing
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>732 bp
ATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAG
ATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTGAAGTGGTGAAC
CGTCGCCTGACGGGCAATAACTTCCATGTTTATCTCAAACCCGATCGGCTGGTGGATCCG
GAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCC
GAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCA
GCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAG
ACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGT
AAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACG
CTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGT
GGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCA
ACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAA
GAGATTGCAGCTCATGAAGCCCGTCTCGATCTGGTGCAGAAGAAAGGCGGAAGTTGCCTC
TGGCGAGCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:243
Protein Molecular Weight:26556
Protein Theoretical pI:7
PDB File:1GZ0
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>23S rRNA (guanosine-2'-O-)-methyltransferase rlmB
MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALESQGVVIQLANRQYLDE
KSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDGVTDPHNLGACLRSADAAG
VHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLARTMRMLQEENIWIVGTAGEADH
TLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPMAGSVSSLNVSVATGICLFEAVR
QRS
References
External Links:
ResourceLink
Uniprot ID:P63177
Uniprot Name:RLMB_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:1786409
PDB ID:1GZ0
CCDB:RLMB_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lovgren, J. M., Wikstrom, P. M. (2001). "The rlmB gene is essential for formation of Gm2251 in 23S rRNA but not for ribosome maturation in Escherichia coli." J Bacteriol 183:6957-6960. Pubmed: 11698387
  • Michel, G., Sauve, V., Larocque, R., Li, Y., Matte, A., Cygler, M. (2002). "The structure of the RlmB 23S rRNA methyltransferase reveals a new methyltransferase fold with a unique knot." Structure 10:1303-1315. Pubmed: 12377117