| Identification |
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| Name: | 23S rRNA (guanosine-2'-O-)-methyltransferase rlmB |
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| Synonyms: | - 23S rRNA Gm2251 2'-O-methyltransferase
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| Gene Name: | rlmB |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | Translation, ribosomal structure and biogenesis |
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| Specific Function: | Specifically methylates the ribose of guanosine 2251 in 23S rRNA |
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| Cellular Location: | Cytoplasmic |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | Not Available |
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| Metabolites: | | ECMDB ID | Name | View |
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| ECMDB00939 | S-Adenosylhomocysteine | MetaboCard |
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| GO Classification: | | Function |
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| binding | | catalytic activity | | methyltransferase activity | | nucleic acid binding | | RNA binding | | RNA methyltransferase activity | | transferase activity | | transferase activity, transferring one-carbon groups | | Process |
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| cellular macromolecule metabolic process | | macromolecule metabolic process | | metabolic process | | RNA metabolic process | | RNA processing |
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| Gene Properties |
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| Blattner: | Not Available |
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| Gene Orientation | Not Available |
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| Centisome Percentage: | Not Available |
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| Left Sequence End | Not Available |
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| Right Sequence End | Not Available |
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| Gene Sequence: | >732 bp
ATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAG
ATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTGAAGTGGTGAAC
CGTCGCCTGACGGGCAATAACTTCCATGTTTATCTCAAACCCGATCGGCTGGTGGATCCG
GAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCC
GAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCA
GCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAG
ACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGT
AAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACG
CTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGT
GGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCA
ACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAA
GAGATTGCAGCTCATGAAGCCCGTCTCGATCTGGTGCAGAAGAAAGGCGGAAGTTGCCTC
TGGCGAGCATAA |
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| Protein Properties |
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| Pfam Domain Function: | |
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| Protein Residues: | 243 |
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| Protein Molecular Weight: | 26556 |
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| Protein Theoretical pI: | 7 |
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| PDB File: | 1GZ0 |
| Signaling Regions: | |
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| Transmembrane Regions: | |
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| Protein Sequence: | >23S rRNA (guanosine-2'-O-)-methyltransferase rlmB
MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALESQGVVIQLANRQYLDE
KSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDGVTDPHNLGACLRSADAAG
VHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLARTMRMLQEENIWIVGTAGEADH
TLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPMAGSVSSLNVSVATGICLFEAVR
QRS |
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| References |
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| External Links: | |
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| General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Lovgren, J. M., Wikstrom, P. M. (2001). "The rlmB gene is essential for formation of Gm2251 in 23S rRNA but not for ribosome maturation in Escherichia coli." J Bacteriol 183:6957-6960. Pubmed: 11698387
- Michel, G., Sauve, V., Larocque, R., Li, Y., Matte, A., Cygler, M. (2002). "The structure of the RlmB 23S rRNA methyltransferase reveals a new methyltransferase fold with a unique knot." Structure 10:1303-1315. Pubmed: 12377117
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