Siroheme synthase (P0AEA8)

Identification
Name:Siroheme synthase
Synonyms:
  • Uroporphyrinogen-III C-methyltransferase
  • Urogen III methylase
  • SUMT
  • Uroporphyrinogen III methylase
  • UROM
  • Precorrin-2 dehydrogenase
  • Sirohydrochlorin ferrochelatase
Gene Name:cysG
Enzyme Class:
Biological Properties
General Function:Involved in methyltransferase activity
Specific Function:Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD- dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
2.0Thumb+1.0Thumb → 2.0Thumb+1.0Thumb+1.0Thumb
2.0S-Adenosylmethionine + 1.0Uroporphyrinogen III → 2.0S-Adenosylhomocysteine + 1.0Precorrin 2 + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Precorrin 2 + 1.0NAD → 1.0Hydrogen ion + 1.0NADH + 1.0Sirohydrochlorin
ReactionCard
1.0Thumb+2.0Thumb ↔ 1.0Fe2++1.0Thumb
1.0Siroheme + 2.0Hydrogen ion ↔ 1.0Fe2+ + 1.0Sirohydrochlorin
ReactionCard
2.0Thumb+1.0Thumb ↔ 2.0Thumb+1.0Thumb
2.0S-Adenosylmethionine + 1.0Uroporphyrinogen III ↔ 2.0S-Adenosylhomocysteine + 1.0Precorrin 2
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Precorrin 2 + 1.0NAD ↔ 1.0Sirohydrochlorin + 1.0NADH + 1.0Hydrogen ion
ReactionCard
2.0Thumb+1.0Thumb+1.0Thumb ↔ 2.0Thumb+1.0Thumb
2.0S-Adenosylmethionine + 1.0Uroporphyrinogen III + 1.0Precorrin-1 ↔ 2.0S-Adenosylhomocysteine + 1.0Precorrin 2
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0S-adenosyl-L-methionine → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Uroporphyrinogen III + 1.0S-adenosyl-L-methionine → 1.0Hydrogen ion + 1.0S-Adenosylhomocysteine + 1.0Precorrin-1
ReactionCard
1.0Precorrin-2+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Precorrin-2 + 1.0NAD → 1.0NADH + 1.0Hydrogen ion + 1.0Sirohydrochlorin
ReactionCard
1.0Thumb+1.0Thumb → 2.0Thumb+1.0Thumb
1.0Sirohydrochlorin + 1.0Iron → 2.0Hydrogen ion + 1.0Siroheme
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+2.0Thumb
1.0Precorrin 2 + 1.0NAD ↔ 1.0Sirohydrochlorin + 1.0NADH + 2.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 3.0Thumb+1.0Thumb
1.0Iron + 1.0Sirohydrochlorin → 3.0Hydrogen ion + 1.0Siroheme
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Precorrin 2 + 1.0NAD → 1.0Hydrogen ion + 1.0NADH + 1.0Sirohydrochlorin
ReactionCard
Complex Reactions:
1.0Thumb+2.0Thumb → 1.0Thumb+1.0Thumb
1.0Siroheme + 2.0Hydrogen ion → 1.0Sirohydrochlorin + 1.0Iron
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB23899Fe2+MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00692IronMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB20183Precorrin 2MetaboCard
ECMDB21568Precorrin-1MetaboCard
ECMDB00939S-AdenosylhomocysteineMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
ECMDB20194SirohemeMetaboCard
ECMDB20195SirohydrochlorinMetaboCard
ECMDB00916Uroporphyrin IIIMetaboCard
ECMDB01086Uroporphyrinogen IIIMetaboCard
GO Classification:
Function
binding
catalytic activity
methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
metabolic process
nitrogen compound metabolic process
oxidation reduction
porphyrin biosynthetic process
porphyrin metabolic process
tetrapyrrole metabolic process
Gene Properties
Blattner:b3368
Gene OrientationClockwise
Centisome Percentage:75.35
Left Sequence End3495850
Right Sequence End3497223
Gene Sequence:
>1374 bp
ATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCA
CTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCC
GGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGT
GCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAG
GAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTACTGCTTTGAAATCCTT
ATTGTCGCCATCGCCGATGTGACCGCTTTTGGTATCTATATGGGTGTCTGGTTCCCGACG
GTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATG
AGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATC
ATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAA
CCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGC
ATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATT
ACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCG
ATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGG
AATCAGGTTGGCACTGCCGGTAGCCCGTTCGTGCTGACGTTCCAGCATATGGGCATTACC
TTTGCCGCCAGCATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGT
GATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAA
ATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACC
GCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATC
GCTTCGCTGGCAACCTTCGCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATT
GCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTGCCGGGT
GGGGTAGCAACGACCATCGGCGGGCTGATTTTCCTGCTCTTTATTATCGGGTTGATTGGT
TATCACCCGGATACGCGTATCTCGCTGTATGTCGGTTTCGCGTGGATTGTTGTGCTGTTG
ATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:457
Protein Molecular Weight:49951
Protein Theoretical pI:6
PDB File:1PJT
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Siroheme synthase
MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALAFIPQFTAWADAGMLTL
VEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNVVDAPKAASFIMPSIIDRS
PLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQLRGRVKQQFATMGERRRFWE
KLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEVVLVGAGPGDAGLLTLKGLQQIQ
QADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHCVPQEEINQILLREAQKGKRVVRLK
GGDPFIFGRGGEELETLCNAGIPFSVVPGITAASGCSAYSGIPLTHRDYAQSVRLITGHL
KTGGELDWENLAAEKQTLVFYMGLNQAATIQQKLIEHGMPGEMPVAIVENGTAVTQRVID
GTLTQLGELAQQMNSPSLIIIGRVVGLRDKLNWFSNH
References
External Links:
ResourceLink
Uniprot ID:P0AEA8
Uniprot Name:CYSG_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674542
PDB ID:1PJT
Ecogene ID:EG10188
Ecocyc:EG10188
ColiBase:b3368
Kegg Gene:b3368
EchoBASE ID:EB0185
CCDB:CYSG_ECOLI
BacMap:16131246
General Reference:
  • Bell, A. I., Gaston, K. L., Cole, J. A., Busby, S. J. (1989). "Cloning of binding sequences for the Escherichia coli transcription activators, FNR and CRP: location of bases involved in discrimination between FNR and CRP." Nucleic Acids Res 17:3865-3874. Pubmed: 2543955
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Peakman, T., Crouzet, J., Mayaux, J. F., Busby, S., Mohan, S., Harborne, N., Wootton, J., Nicolson, R., Cole, J. (1990). "Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome." Eur J Biochem 191:315-323. Pubmed: 2200672
  • Spencer, J. B., Stolowich, N. J., Roessner, C. A., Scott, A. I. (1993). "The Escherichia coli cysG gene encodes the multifunctional protein, siroheme synthase." FEBS Lett 335:57-60. Pubmed: 8243665
  • Warren, M. J., Roessner, C. A., Santander, P. J., Scott, A. I. (1990). "The Escherichia coli cysG gene encodes S-adenosylmethionine-dependent uroporphyrinogen III methylase." Biochem J 265:725-729. Pubmed: 2407234
  • Warren, M. J., Stolowich, N. J., Santander, P. J., Roessner, C. A., Sowa, B. A., Scott, A. I. (1990). "Enzymatic synthesis of dihydrosirohydrochlorin (precorrin-2) and of a novel pyrrocorphin by uroporphyrinogen III methylase." FEBS Lett 261:76-80. Pubmed: 2407558