| Identification |
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| Name: | 23S rRNA (uracil-5-)-methyltransferase rumA |
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| Synonyms: | - 23S rRNA(M-5-U1939)-methyltransferase
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| Gene Name: | rumA |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | Translation, ribosomal structure and biogenesis |
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| Specific Function: | Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA |
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| Cellular Location: | Cytoplasmic |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | Not Available |
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| Metabolites: | | ECMDB ID | Name | View |
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| ECMDB00939 | S-Adenosylhomocysteine | MetaboCard |
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| GO Classification: | | Function |
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| binding | | catalytic activity | | methyltransferase activity | | nucleic acid binding | | RNA binding | | RNA methyltransferase activity | | transferase activity | | transferase activity, transferring one-carbon groups | | Process |
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| cellular macromolecule metabolic process | | macromolecule metabolic process | | metabolic process | | RNA metabolic process | | RNA processing |
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| Gene Properties |
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| Blattner: | Not Available |
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| Gene Orientation | Not Available |
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| Centisome Percentage: | Not Available |
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| Left Sequence End | Not Available |
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| Right Sequence End | Not Available |
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| Gene Sequence: | >1302 bp
ATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTT
ATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAGTTGCCGCAA
AGTAGCCTGCTTACGCTCACGCAATATGCTTTTCTTGCTACCGCACTGGCCTGCTTCGCT
CAGGCGTTTTGCGGTCATCGTCGCGCTATTATGGAAGGGCCAGGTGGCCTGTGGTGGGGA
ACCATCCTTACTATCACCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGCC
ACCAGCCTGGCAGTGGGGATTGCACTCTCCGGCGTGCTGACGATGTTGATTGGTTTTAGC
GGATTAGGCCATCGCCTGGCACGGTTATTTACGCCGTCGGTGATGGTCTTGTTTATGTTG
ATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGC
ATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATG
TGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTG
GTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTC
TCCGGTGAGTTGCACTGGCAGTGGTTCCCGCTCGGCAGTGGCGGTGCTTTGTCGCCGGGA
ATTATTCTGACGGCGGTGATTACAGGTCTGGTAAATATCAGCAATACCTACGGTGCGATT
CGGGGCACGGATGTTTTTTATCCGCAGCAGGGCGCAGGGAATACGCGTTATCGTCGTAGC
TTTGTGGCGACCGGATTTATGACGCTGATAACCGTACCGCTGGCGGTAATTCCATTTTCA
CCGTTTGTTTCATCCATTGGTTTATTAACCCAGACTGGCGATTACACGCGGCGTTCGTTT
ATTTATGGCAGCGTTATTTGCCTGCTGGTGGCGCTGGTTCCTGCACTCACGCGACTGTTT
TGCAGTATCCCTTTACCCGTGAGTAGTGCGGTCATGCTGGTTTCTTATCTGCCTTTACTC
TTTTCCGCGCTGGTGTTTAGCCAGCAAATAACGTTTACCGCTCGCAATATTTATCGACTC
GCATTGCCGTTATTTGTCGGCATATTTTTAATGGCATTACCGCCTGTGTATCTGCAAGAC
CTTCCATTAACGCTTCGTCCTCTGCTCAGTAACGGCTTATTGGTCGGGATTTTACTGGCT
GTTCTTATGGATAACCTTATTCCGTGGGAACGCATCGAATAA |
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| Protein Properties |
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| Pfam Domain Function: | |
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| Protein Residues: | 433 |
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| Protein Molecular Weight: | 48052 |
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| Protein Theoretical pI: | 9 |
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| PDB File: | 2BH2 |
| Signaling Regions: | |
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| Transmembrane Regions: | |
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| Protein Sequence: | >23S rRNA (uracil-5-)-methyltransferase rumA
MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGLLPQENAEVTVTEDKKQ
YARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQRSKSAALARLMKHDVSEVI
ADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVKQCPILAPQLEALLPKVRACL
GSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREKLERFSHSEGLDLYLAPDSEILE
TVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVARALEWLDVQPEDRVLDLFCGMGNF
TLPLATQAASVVGVEGVPALVEKGQQNARLNGLQNVTFYHENLEEDVTKQPWAKNGFDKV
LLDPARAGAAGVMQQIIKLEPIRIVYVSCNPATLARDSEALLKAGYTIARLAMLDMFPHT
GHLESMVLFSRVK |
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| References |
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| External Links: | |
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| General Reference: | - Agarwalla, S., Kealey, J. T., Santi, D. V., Stroud, R. M. (2002). "Characterization of the 23 S ribosomal RNA m5U1939 methyltransferase from Escherichia coli." J Biol Chem 277:8835-8840. Pubmed: 11779873
- Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Lee, T. T., Agarwalla, S., Stroud, R. M. (2004). "Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase." Structure 12:397-407. Pubmed: 15016356
- Lee, T. T., Agarwalla, S., Stroud, R. M. (2005). "A unique RNA Fold in the RumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function." Cell 120:599-611. Pubmed: 15766524
- Madsen, C. T., Mengel-Jorgensen, J., Kirpekar, F., Douthwaite, S. (2003). "Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry." Nucleic Acids Res 31:4738-4746. Pubmed: 12907714
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