Putative uroporphyrinogen-III C-methyltransferase (P09127)

Identification
Name:Putative uroporphyrinogen-III C-methyltransferase
Synonyms:
  • Urogen-III methylase
  • ORF X
Gene Name:hemX
Enzyme Class:
Biological Properties
General Function:Coenzyme transport and metabolism
Specific Function:S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1
Cellular Location:Cell inner membrane; Single-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
2.0Thumb+1.0Thumb → 2.0Thumb+1.0Thumb+1.0Thumb
2.0S-Adenosylmethionine + 1.0Uroporphyrinogen III → 2.0S-Adenosylhomocysteine + 1.0Precorrin 2 + 1.0Hydrogen ion
ReactionCard
2.0Thumb+1.0Thumb ↔ 2.0Thumb+1.0Thumb
2.0S-Adenosylmethionine + 1.0Uroporphyrinogen III ↔ 2.0S-Adenosylhomocysteine + 1.0Precorrin 2
ReactionCard
2.0Thumb+1.0Thumb+1.0Thumb ↔ 2.0Thumb+1.0Thumb
2.0S-Adenosylmethionine + 1.0Uroporphyrinogen III + 1.0Precorrin-1 ↔ 2.0S-Adenosylhomocysteine + 1.0Precorrin 2
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB20183Precorrin 2MetaboCard
ECMDB21568Precorrin-1MetaboCard
ECMDB00939S-AdenosylhomocysteineMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
ECMDB00916Uroporphyrin IIIMetaboCard
ECMDB01086Uroporphyrinogen IIIMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b3803
Gene OrientationCounterclockwise
Centisome Percentage:85.91
Left Sequence End3985908
Right Sequence End3987089
Gene Sequence:
>1182 bp
ATGACCTGTTCAACTTCATTAAGCGGCAAAAACAGGATTGTCCTTATCGCTGGCATTCTG
ATGATTGCCACAACATTACGCGTCACCTTTACCGGCGCAGCACCGTTACTGGATACGATT
CGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTG
GCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGT
AGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCG
CCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTC
TTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCA
TATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCT
TTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCT
CTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGC
GCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTT
GGGATCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGT
CACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCA
GCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCA
GCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCAC
GCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTG
ACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCA
CAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGAT
GCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCG
ATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:393
Protein Molecular Weight:42963
Protein Theoretical pI:4
Signaling Regions:
  • None
Transmembrane Regions:
  • 37-57
Protein Sequence:
>Putative uroporphyrinogen-III C-methyltransferase
MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAIAIALAAGIGLYGWGKQ
QAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLKQANRQQETLAKQLDEVQQ
KVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAALLKSADASLADMNDPSLITV
RRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRLADNDSDGSPMDSDGEELSSSIS
EWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQDIYLRENIRSRLLVAAQAVPRHQEE
TYRQALENVSTWVRAYYDTDDATTKAFLDEVDQLSQQNISMDLPETLQSQAMLEKLMQTR
VRNLLAQPAAGTTEAKPAPAPQADTPAAAPQGE
References
External Links:
ResourceLink
Uniprot ID:P09127
Uniprot Name:HEMX_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1736416
Ecogene ID:EG10433
Ecocyc:EG10433
ColiBase:b3803
Kegg Gene:b3803
EchoBASE ID:EB0428
CCDB:HEMX_ECOLI
BacMap:16131655
General Reference:
  • Alefounder, P. R., Abell, C., Battersby, A. R. (1988). "The sequence of hemC, hemD and two additional E. coli genes." Nucleic Acids Res 16:9871. Pubmed: 3054815
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Sasarman, A., Echelard, Y., Letowski, J., Tardif, D., Drolet, M. (1988). "Nucleotide sequence of the hemX gene, the third member of the Uro operon of Escherichia coli K12." Nucleic Acids Res 16:11835. Pubmed: 3062586
  • Stenberg, F., Chovanec, P., Maslen, S. L., Robinson, C. V., Ilag, L. L., von Heijne, G., Daley, D. O. (2005). "Protein complexes of the Escherichia coli cell envelope." J Biol Chem 280:34409-34419. Pubmed: 16079137