Ribosomal RNA small subunit methyltransferase B (P36929)

Identification
Name:Ribosomal RNA small subunit methyltransferase B
Synonyms:
  • 16S rRNA m5C967 methyltransferase
  • rRNA (cytosine-C(5)-)-methyltransferase rsmB
Gene Name:rsmB
Enzyme Class:
Biological Properties
General Function:Defense mechanisms
Specific Function:Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA
Cellular Location:Cytoplasm (Potential)
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
GO Classification:
Function
binding
catalytic activity
methyltransferase activity
nucleic acid binding
RNA binding
RNA methyltransferase activity
rRNA methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
biological regulation
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
regulation of biological process
regulation of gene expression
regulation of macromolecule metabolic process
regulation of metabolic process
regulation of transcription
regulation of transcription, DNA-dependent
RNA metabolic process
rRNA metabolic process
rRNA processing
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>1290 bp
ATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCC
GCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCC
GGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCA
GGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTA
ACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACAC
GCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAG
GCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAG
GTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGAT
GGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCAT
TACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTT
AATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTG
ATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCATATCCTTGGGGCTGGTTTGTGGA
TTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAA
CACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATG
GTGCCGGAAGGCGGTCTGGCAATGGTGCCGCAACTGGTTAACGAGGGCGTGATGATCGTT
GGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCC
ATTGCATCGGCTCAGGCTGCCGCCACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTC
TCCGCCAGCAGTCTGGCGCAATACAAACGTGAGCTGGAACAAAGCTGCGTCATGCGTGAT
ATGCAGCATTTTCGCAAGATCCCGGCGCTGATGGAAAACCCGCGCCTGTTTAGCCAATAC
CCACGAATGGTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAG
CCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAA
GATGGCATTAAGGGAGCAACCGCGCTATGA
Protein Properties
Pfam Domain Function:
Protein Residues:429
Protein Molecular Weight:48347
Protein Theoretical pI:8
PDB File:1SQG
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ribosomal RNA small subunit methyltransferase B
MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALLQELCFGVLRTLSQLDW
LINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAETVEGAIAIKRPQLKGLING
VLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQWQSIVEANNQRPPMWLRINR
THHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVHALPGFEDGWVTVQDASAQGCMT
WLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAVDIDEQRLSRVYDNLKRLGMKATVK
QGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRHPDIKWLRRDRDIPELAQLQSEILDAI
WPHLKTGGTLVYATCSVLPEENSLQIKAFLQRTADAELCETGTPEQPGKQNLPGAEEGDG
FFYAKLIKK
References
External Links:
ResourceLink
Uniprot ID:P36929
Uniprot Name:RSMB_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:1787991
PDB ID:1SQG
CCDB:RSMB_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Foster, P. G., Nunes, C. R., Greene, P., Moustakas, D., Stroud, R. M. (2003). "The first structure of an RNA m5C methyltransferase, Fmu, provides insight into catalytic mechanism and specific binding of RNA substrate." Structure 11:1609-1620. Pubmed: 14656444
  • Gu, X. R., Gustafsson, C., Ku, J., Yu, M., Santi, D. V. (1999). "Identification of the 16S rRNA m5C967 methyltransferase from Escherichia coli." Biochemistry 38:4053-4057. Pubmed: 10194318
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Liu, Y., Santi, D. V. (2000). "m5C RNA and m5C DNA methyl transferases use different cysteine residues as catalysts." Proc Natl Acad Sci U S A 97:8263-8265. Pubmed: 10899996
  • Mazel, D., Coic, E., Blanchard, S., Saurin, W., Marliere, P. (1997). "A survey of polypeptide deformylase function throughout the eubacterial lineage." J Mol Biol 266:939-949. Pubmed: 9086272
  • Meinnel, T., Guillon, J. M., Mechulam, Y., Blanquet, S. (1993). "The Escherichia coli fmt gene, encoding methionyl-tRNA(fMet) formyltransferase, escapes metabolic control." J Bacteriol 175:993-1000. Pubmed: 8432722
  • Schlosser, A., Hamann, A., Bossemeyer, D., Schneider, E., Bakker, E. P. (1993). "NAD+ binding to the Escherichia coli K(+)-uptake protein TrkA and sequence similarity between TrkA and domains of a family of dehydrogenases suggest a role for NAD+ in bacterial transport." Mol Microbiol 9:533-543. Pubmed: 8412700
  • Tscherne, J. S., Nurse, K., Popienick, P., Michel, H., Sochacki, M., Ofengand, J. (1999). "Purification, cloning, and characterization of the 16S RNA m5C967 methyltransferase from Escherichia coli." Biochemistry 38:1884-1892. Pubmed: 10026269