Cyclopropane-fatty-acyl-phospholipid synthase (P0A9H7)

Identification
Name:Cyclopropane-fatty-acyl-phospholipid synthase
Synonyms:
  • CFA synthase
  • Cyclopropane fatty acid synthase
Gene Name:cfa
Enzyme Class:
Biological Properties
General Function:Involved in lipid biosynthetic process
Specific Function:Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge
Cellular Location:Cytoplasm
SMPDB Pathways:
  • Cyclopropane Fatty Acid (CFA) Biosynthesis PW002109
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Phospholipid cyclopropane fatty acid
1.0S-Adenosylmethionine + 1.0Phospholipid olefinic fatty acid ↔ 1.0S-Adenosylhomocysteine + 1.0Phospholipid cyclopropane fatty acid
ReactionCard
SMPDB Reactions:
1.0a [phospholipid] olefinic fatty acid+1.0S-adenosyl-L-methionine → 1.0a [phospholipid] cyclopropane fatty acid+1.0Thumb+1.0Thumb
1.0a [phospholipid] olefinic fatty acid + 1.0S-adenosyl-L-methionine → 1.0a [phospholipid] cyclopropane fatty acid + 1.0S-Adenosylhomocysteine + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
2.0Thumb+1.0Thumb → 2.0Thumb+1.0Thumb+2.0Thumb
2.0S-Adenosylmethionine + 1.0PE(14:0/14:0) → 2.0S-Adenosylhomocysteine + 1.0Cyclopropane phosphatidylethanolamine (dihexadec-9,10-cyclo-anoyl, N-C16:0 cyclo) + 2.0Hydrogen ion
ReactionCard
2.0Thumb+1.0Thumb → 2.0Thumb+1.0Thumb+2.0Thumb
2.0S-Adenosylmethionine + 1.0PE(14:0/14:0) → 2.0S-Adenosylhomocysteine + 1.0Cyclopropane phosphatidylethanolamine (dioctadec-11,12-cyclo-anoyl, N-C18:0 cyclo) + 2.0Hydrogen ion
ReactionCard
2.0Thumb+1.0Thumb → 2.0Thumb+1.0Thumb+2.0Thumb
2.0S-Adenosylmethionine + 1.0PG(16:1(9Z)/16:1(9Z)) → 2.0S-Adenosylhomocysteine + 1.0Cyclopropane phosphatidylglycerol (dihexadec-9,10-cyclo-anoyl, N-C16:0 cyclo) + 2.0Hydrogen ion
ReactionCard
2.0Thumb+1.0Thumb → 2.0Thumb+1.0Thumb+2.0Thumb
2.0S-Adenosylmethionine + 1.0PG(18:1(11Z)/18:1(11Z)) → 2.0S-Adenosylhomocysteine + 1.0Cyclopropane phosphatidylglycerol (dioctadec-11,12-cyclo-anoyl, N-C18:0 cyclo) + 2.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21322Cyclopropane phosphatidylethanolamine (dihexadec-9,10-cyclo-anoyl, N-C16:0 cyclo)MetaboCard
ECMDB21323Cyclopropane phosphatidylethanolamine (dioctadec-11,12-cyclo-anoyl, N-C18:0 cyclo)MetaboCard
ECMDB21324Cyclopropane phosphatidylglycerol (dihexadec-9,10-cyclo-anoyl, N-C16:0 cyclo)MetaboCard
ECMDB21325Cyclopropane phosphatidylglycerol (dioctadec-11,12-cyclo-anoyl, N-C18:0 cyclo)MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB08821PE(14:0/14:0)MetaboCard
ECMDB10586PG(16:1(9Z)/16:1(9Z))MetaboCard
ECMDB10618PG(18:1(11Z)/18:1(11Z))MetaboCard
ECMDB00939S-AdenosylhomocysteineMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
GO Classification:
Process
lipid biosynthetic process
lipid metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b1661
Gene OrientationClockwise
Centisome Percentage:37.49
Left Sequence End1739437
Right Sequence End1740585
Gene Sequence:
>1149 bp
TTGATTAAGTCAGCGCTATTGGTTCTGGAAGACGGAACCCAGTTTCACGGTCGGGCCATA
GGGGCAACAGGTTCGGCGGTTGGGGAAGTCGTTTTCAATACTTCAATGACCGGTTATCAA
GAAATCCTCACTGATCCTTCCTATTCTCGTCAAATCGTTACTCTTACTTATCCCCATATT
GGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTG
GTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCT
TACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGACGCGT
TTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCG
GCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAA
GAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGC
CTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGT
GCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCG
GCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAAC
GGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAA
ACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGT
GCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTG
GAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACA
TTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATT
CATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCA
CACGACGCCGCGCCGTTGTTCGACCACTTTATCGAGTTAATTGAGCAGTACCGTAAAACC
GCTAAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:382
Protein Molecular Weight:43909
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Cyclopropane-fatty-acyl-phospholipid synthase
MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNPDFFKRVLQEGSLGLGE
SYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGARLFNLQSKKRAWIVGKEHY
DLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMICEKLQLKPGMRVLDIGCGWGG
LAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTILLQDYRDLNDQFDRIVSVGMFEH
VGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLNVDPWINKYIFPNGCLPSVRQIAQS
SEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPEIADNYSERFKRMFTYYLNACAGAFRA
RDIQLWQVVFSRGVENGLRVAR
References
External Links:
ResourceLink
Uniprot ID:P0A9H7
Uniprot Name:CFA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321914
Ecogene ID:EG11531
Ecocyc:EG11531
ColiBase:b1661
Kegg Gene:b1661
EchoBASE ID:EB1493
CCDB:CFA_ECOLI
BacMap:16129619
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Wang, A. Y., Grogan, D. W., Cronan, J. E. Jr (1992). "Cyclopropane fatty acid synthase of Escherichia coli: deduced amino acid sequence, purification, and studies of the enzyme active site." Biochemistry 31:11020-11028. Pubmed: 1445840