ECMDB

Identification

Name:

Ribosomal RNA small subunit methyltransferase E

Synonyms:

16S rRNA m3U1498 methyltransferase

Gene Name:

rsmE

Enzyme Class:

2.1.1.193

Biological Properties

General Function:

Involved in rRNA (uridine-N3-)-methyltransferase activity

Specific Function:

Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit

Cellular Location:

Cytoplasm (Probable)

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

Metabolites:

ECMDB ID	Name	View
ECMDB00939	S-Adenosylhomocysteine	MetaboCard

GO Classification:

Function
catalytic activity
methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
rRNA metabolic process
rRNA processing

Gene Properties

Blattner:

Not Available

Gene Orientation

Not Available

Centisome Percentage:

Not Available

Left Sequence End

Not Available

Right Sequence End

Not Available

Gene Sequence:

>732 bp
ATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAG
ATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTGAAGTGGTGAAC
CGTCGCCTGACGGGCAATAACTTCCATGTTTATCTCAAACCCGATCGGCTGGTGGATCCG
GAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCC
GAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCA
GCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAG
ACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGT
AAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACG
CTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGT
GGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCA
ACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAA
GAGATTGCAGCTCATGAAGCCCGTCTCGATCTGGTGCAGAAGAAAGGCGGAAGTTGCCTC
TGGCGAGCATAA

Protein Properties

Pfam Domain Function:

Methyltrans_RNA (PF04452 )

Protein Residues:

243

Protein Molecular Weight:

26978

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Ribosomal RNA small subunit methyltransferase E
MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEITSASKK
SVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLD
SERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLHPRASNSIN
TLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITALQVRFG
DLG

References

External Links:

Resource	Link
Uniprot ID:	P0AGL7
Uniprot Name:	RSME_ECOLI
GenBank Gene ID:	U00096
Genebank Protein ID:	1786409
CCDB:	RSME_ECOLI

General Reference:

Basturea, G. N., Deutscher, M. P. (2007). "Substrate specificity and properties of the Escherichia coli 16S rRNA methyltransferase, RsmE." RNA 13:1969-1976. Pubmed: 17872509
Basturea, G. N., Rudd, K. E., Deutscher, M. P. (2006). "Identification and characterization of RsmE, the founding member of a new RNA base methyltransferase family." RNA 12:426-434. Pubmed: 16431987
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Gushima, H., Yasuda, S., Soeda, E., Yokota, M., Kondo, M., Kimura, A. (1984). "Complete nucleotide sequence of the E. coli glutathione synthetase gsh-II." Nucleic Acids Res 12:9299-9307. Pubmed: 6393055
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Jekel, M., Wackernagel, W. (1994). "Location of the endA gene coding for endonuclease I on the physical map of the Escherichia coli K-12 chromosome." J Bacteriol 176:1550-1551. Pubmed: 8113204

Ribosomal RNA small subunit methyltransferase E (P0AGL7)