Identification
Name:Uncharacterized adenine-specific methylase yhdJ
Synonyms:Not Available
Gene Name:yhdJ
Enzyme Class:
Biological Properties
General Function:Involved in DNA binding
Specific Function:S-adenosyl-L-methionine + DNA adenine = S- adenosyl-L-homocysteine + DNA 6-methylaminopurine
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0DNA adenine1.0Thumb+1.0DNA 6-methylaminopurine
1.0S-Adenosylmethionine + 1.0DNA adenine ↔ 1.0S-Adenosylhomocysteine + 1.0DNA 6-methylaminopurine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
GO Classification:
Function
binding
catalytic activity
DNA binding
methyltransferase activity
N-methyltransferase activity
nucleic acid binding
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular macromolecule metabolic process
cellular metabolic process
DNA alkylation
DNA metabolic process
DNA methylation
DNA modification
macromolecule metabolic process
metabolic process
methylation
one-carbon metabolic process
Gene Properties
Blattner:b3262
Gene OrientationClockwise
Centisome Percentage:73.49
Left Sequence End3409675
Right Sequence End3410559
Gene Sequence:
>885 bp
ATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCT
TCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAACTTATCGACGTTTCCACCTGTATC
GGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGG
CACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATG
CGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGT
ATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAG
TACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCATT
GCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGC
ACGCTCTGCGTCGATCGCGTCAGCGTCGGCCAGGAACCGGCTTGTGTGAAAACCTGTCCG
ACCGGGGCTATCCACTTCGGCACCAAGAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTG
GCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGT
GGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTG
CCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTG
GCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGC
CCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:294
Protein Molecular Weight:33397
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Uncharacterized adenine-specific methylase yhdJ
MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYNIGKNFDGLIEAWKEDL
FIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLFTIKSRIVWSYDSSGVQAK
KHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALIDYRKNPPQPYNHQKVPGNVW
DFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIVLDPFAGSFTTGAVAIASGRKFI
GIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTGNLSKRSRLSEVDPDLITK
References
External Links:
ResourceLink
Uniprot ID:P28638
Uniprot Name:YHDJ_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742408
Ecogene ID:EG11498
Ecocyc:EG11498
ColiBase:b3262
Kegg Gene:b3262
EchoBASE ID:EB1461
CCDB:YHDJ_ECOLI
BacMap:90111566
General Reference:
  • Ball, C. A., Osuna, R., Ferguson, K. C., Johnson, R. C. (1992). "Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli." J Bacteriol 174:8043-8056. Pubmed: 1459953
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Johnson, R. C., Ball, C. A., Pfeffer, D., Simon, M. I. (1988). "Isolation of the gene encoding the Hin recombinational enhancer binding protein." Proc Natl Acad Sci U S A 85:3484-3488. Pubmed: 2835774