Ribosomal RNA large subunit methyltransferase I (P75876)

Identification
Name:Ribosomal RNA large subunit methyltransferase I
Synonyms:
  • 23S rRNA m5C1962 methyltransferase
  • rRNA (cytosine-C(5)-)-methyltransferase rlmI
Gene Name:rlmI
Enzyme Class:
Biological Properties
General Function:Involved in RNA binding
Specific Function:Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA. Methylation occurs before assembly of 23S rRNA into 50S subunits
Cellular Location:Cytoplasm (Potential)
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
GO Classification:
Function
binding
nucleic acid binding
RNA binding
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>1191 bp
ATGAGTGTACGTTTAGTGTTAGCCAAAGGGCGCGAAAAATCATTACTTCGTCGCCATCCG
TGGGTCTTTTCCGGGGCCGTTGCCCGCATGGAAGGTAAAGCCAGCCTCGGTGAAACCATC
GATATTGTTGATCATCAGGGAAAATGGTTAGCACGCGGCGCTTATTCGCCAGCTTCGCAA
ATCCGGGCGCGCGTCTGGACGTTTGACCCGTCTGAGTCTATCGACATTGCTTTTTTTTCC
CGCCGTTTGCAACAAGCACAAAAATGGCGTGACTGGCTGGCGCAAAAAGATGGCCTCGAC
AGCTATCGTTTAATCGCCGGAGAATCTGATGGCCTGCCGGGTATTACTATCGATCGTTTC
GGTAATTTTCTGGTGCTGCAACTGCTGAGTGCTGGGGCAGAATATCAGCGCGCGGCATTA
ATTAGTGCCCTGCAAACGCTGTACCCGGAATGTTCGATTTACGATCGCAGCGACGTCGCG
GTACGTAAAAAAGAAGGAATGGAGCTGACCCAGGGCCCCGTCACCGGCGAGTTGCCACCT
GCCCTGCTGCCGATTGAAGAACACGGAATGAAACTGCTGGTGGATATTCAGCACGGACAC
AAAACGGGCTACTACCTGGACCAGCGTGATAGCCGCCTGGCTACCCGCCGCTACGTTGAA
AATAAACGTGTGCTGAACTGTTTCTCCTATACCGGTGGTTTCGCCGTATCGGCACTGATG
GGCGGTTGCAGCCAGGTTGTCAGCGTTGATACCTCCCAGGAAGCGCTGGATATTGCACGG
CAGAACGTTGAGCTGAACAAACTGGATCTGAGCAAGGCTGAGTTTGTCCGTGATGATGTC
TTTAAATTGCTGCGTACTTATCGCGATCGCGGTGAAAAATTTGACGTTATCGTGATGGAC
CCGCCGAAGTTTGTTGAGAATAAAAGCCAGTTGATGGGCGCGTGTCGTGGTTATAAAGAC
ATCAACATGCTGGCGATTCAGCTGCTGAATGAAGGCGGTATTCTCCTGACTTTCTCCTGT
TCCGGTCTGATGACCAGCGATTTATTTCAGAAAATCATCGCGGATGCCGCAATTGATGCC
GGTCGTGATGTACAATTTATAGAGCAGTTCCGTCAGGCAGCCGATCATCCGGTGATCGCT
ACCTATCCGGAAGGGCTATATCTGAAAGGGTTTGCCTGTCGCGTCATGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:396
Protein Molecular Weight:44357
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ribosomal RNA large subunit methyltransferase I
MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVDHQGKWLARGAYSPASQ
IRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSYRLIAGESDGLPGITIDRF
GNFLVLQLLSAGAEYQRAALISALQTLYPECSIYDRSDVAVRKKEGMELTQGPVTGELPP
ALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTGGFAVSALM
GGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFVRDDVFKLLRTYRDRGEKFDVIVMD
PPKFVENKSQLMGACRGYKDINMLAIQLLNEGGILLTFSCSGLMTSDLFQKIIADAAIDA
GRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM
References
External Links:
ResourceLink
Uniprot ID:P75876
Uniprot Name:RLMI_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:145693111
CCDB:RLMI_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Purta, E., O'Connor, M., Bujnicki, J. M., Douthwaite, S. (2008). "YccW is the m5C methyltransferase specific for 23S rRNA nucleotide 1962." J Mol Biol 383:641-651. Pubmed: 18786544