ECMDB: tRNA (adenine-N(6)-)-methyltransferase (P31825)

Identification

Name:

tRNA (adenine-N(6)-)-methyltransferase

Synonyms:

tRNA m6A37 methyltransferase

Gene Name:

yfiC

Enzyme Class:

Biological Properties

General Function:

Involved in nucleic acid binding

Specific Function:

Specifically methylates the adenine in position 37 of tRNA(Val)

Cellular Location:

Cytoplasm (Potential)

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

KEGG Reactions:

1.0

1.0tRNA

↔

1.0

1.0tRNA containing N6-methyladenine

1.0S-Adenosylmethionine + 1.0tRNA ↔ 1.0S-Adenosylhomocysteine + 1.0tRNA containing N6-methyladenine
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00939	S-Adenosylhomocysteine	MetaboCard

GO Classification:

Function
binding
catalytic activity
methyltransferase activity
nucleic acid binding
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular metabolic process
metabolic process
methylation
one-carbon metabolic process

Gene Properties

Blattner:

Not Available

Gene Orientation

Not Available

Centisome Percentage:

Not Available

Left Sequence End

Not Available

Right Sequence End

Not Available

Gene Sequence:

>738 bp
ATGTATCCCGTCGACCTTCATATGCATACCGTTGCCAGCACACATGCATATAGCACATTA
AGTGATTACATTGCCCAGGCCAAACAAAAGGGCATTAAACTTTTTGCGATCACCGATCAT
GGCCCGGATATGGAAGATGCGCCGCATCACTGGCACTTCATTAACATGCGTATCTGGCCG
CGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGAT
GGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTT
CATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACT
ATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGAT
GTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCC
TCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTA
CGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGG
GAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTG
AATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCG
GAATTTGCAGACCTTTAA

Protein Properties

Pfam Domain Function:

MTS (PF05175 )

Protein Residues:

245

Protein Molecular Weight:

27270

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>tRNA (adenine-N(6)-)-methyltransferase
MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLAL
MLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLII
SNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECITEEGFFCVVLPEQIGNGFTEL
ALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQA
FYLFM

References

External Links:

Resource	Link
Uniprot ID:	P31825
Uniprot Name:	TRMN6_ECOLI
GenBank Gene ID:	U00096
Genebank Protein ID:	1787271
CCDB:	TRMN6_ECOLI

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Golovina, A. Y., Sergiev, P. V., Golovin, A. V., Serebryakova, M. V., Demina, I., Govorun, V. M., Dontsova, O. A. (2009). "The yfiC gene of E. coli encodes an adenine-N6 methyltransferase that specifically modifies A37 of tRNA1Val(cmo5UAC)." RNA 15:1134-1141. Pubmed: 19383770
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553