| Identification |
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| Name: | tRNA (adenine-N(6)-)-methyltransferase |
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| Synonyms: | - tRNA m6A37 methyltransferase
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| Gene Name: | yfiC |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | Involved in nucleic acid binding |
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| Specific Function: | Specifically methylates the adenine in position 37 of tRNA(Val) |
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| Cellular Location: | Cytoplasm (Potential) |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | Not Available |
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| KEGG Reactions: | |
1.0 | + | 1.0tRNA | ↔ | 1.0 | + | 1.0tRNA containing N6-methyladenine |
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| Metabolites: | | ECMDB ID | Name | View |
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| ECMDB00939 | S-Adenosylhomocysteine | MetaboCard |
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| GO Classification: | | Function |
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| binding | | catalytic activity | | methyltransferase activity | | nucleic acid binding | | transferase activity | | transferase activity, transferring one-carbon groups | | Process |
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| cellular metabolic process | | metabolic process | | methylation | | one-carbon metabolic process |
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| Gene Properties |
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| Blattner: | Not Available |
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| Gene Orientation | Not Available |
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| Centisome Percentage: | Not Available |
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| Left Sequence End | Not Available |
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| Right Sequence End | Not Available |
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| Gene Sequence: | >738 bp
ATGTATCCCGTCGACCTTCATATGCATACCGTTGCCAGCACACATGCATATAGCACATTA
AGTGATTACATTGCCCAGGCCAAACAAAAGGGCATTAAACTTTTTGCGATCACCGATCAT
GGCCCGGATATGGAAGATGCGCCGCATCACTGGCACTTCATTAACATGCGTATCTGGCCG
CGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGAT
GGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTT
CATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACT
ATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGAT
GTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCC
TCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTA
CGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGG
GAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTG
AATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCG
GAATTTGCAGACCTTTAA |
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| Protein Properties |
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| Pfam Domain Function: | |
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| Protein Residues: | 245 |
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| Protein Molecular Weight: | 27270 |
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| Protein Theoretical pI: | 5 |
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| Signaling Regions: | |
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| Transmembrane Regions: | |
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| Protein Sequence: | >tRNA (adenine-N(6)-)-methyltransferase
MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLAL
MLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLII
SNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECITEEGFFCVVLPEQIGNGFTEL
ALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQA
FYLFM |
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| References |
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| External Links: | |
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| General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Golovina, A. Y., Sergiev, P. V., Golovin, A. V., Serebryakova, M. V., Demina, I., Govorun, V. M., Dontsova, O. A. (2009). "The yfiC gene of E. coli encodes an adenine-N6 methyltransferase that specifically modifies A37 of tRNA1Val(cmo5UAC)." RNA 15:1134-1141. Pubmed: 19383770
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
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