tRNA (guanine-N(1)-)-methyltransferase (P0A873)

Identification
Name:tRNA (guanine-N(1)-)-methyltransferase
Synonyms:
  • M1G-methyltransferase
  • tRNA [GM37] methyltransferase
Gene Name:trmD
Enzyme Class:
Biological Properties
General Function:Involved in RNA binding
Specific Function:Specifically methylates guanosine-37 in various tRNAs
Cellular Location:Cytoplasm (Potential)
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
GO Classification:
Function
binding
catalytic activity
methyltransferase activity
nucleic acid binding
RNA binding
RNA methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
tRNA (guanine) methyltransferase activity
tRNA (guanine-N1-)-methyltransferase activity
tRNA methyltransferase activity
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
tRNA metabolic process
tRNA processing
Gene Properties
Blattner:b2607
Gene OrientationCounterclockwise
Centisome Percentage:59.11
Left Sequence End2742594
Right Sequence End2743361
Gene Sequence:
>768 bp
ATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACC
GGGCTTGTACAGCGTGGTGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTT
TACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATC
GCCAAACTTGTCCAGGACTACCCGATAAAATCCTGTTCGGTGGTGGCTCATATTCGCCAG
GCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGG
CGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGC
AACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAA
TTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCC
TCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGC
TATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGC
GTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCGCAGACCACACCGAAT
GATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATT
ATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:255
Protein Molecular Weight:28422
Protein Theoretical pI:6
PDB File:1P9P
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>tRNA (guanine-N(1)-)-methyltransferase
MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHDRHRTVDDRPYGGGPGM
LMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSELATNQKLILVCGRYEGIDE
RVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVLGHEASATEDSFAEGLLDCPH
YTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWLRRPELLENLALTEEQARLLAEF
KTEHAQQQHKHDGMA
References
External Links:
ResourceLink
Uniprot ID:P0A873
Uniprot Name:TRMD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4902959
PDB ID:1P9P
Ecogene ID:EG11023
Ecocyc:EG11023
ColiBase:b2607
Kegg Gene:b2607
EchoBASE ID:EB1016
CCDB:TRMD_ECOLI
BacMap:16130528
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bystrom, A. S., Hjalmarsson, K. J., Wikstrom, P. M., Bjork, G. R. (1983). "The nucleotide sequence of an Escherichia coli operon containing genes for the tRNA(m1G)methyltransferase, the ribosomal proteins S16 and L19 and a 21-K polypeptide." EMBO J 2:899-905. Pubmed: 6357787
  • Elkins, P. A., Watts, J. M., Zalacain, M., van Thiel, A., Vitazka, P. R., Redlak, M., Andraos-Selim, C., Rastinejad, F., Holmes, W. M. (2003). "Insights into catalysis by a knotted TrmD tRNA methyltransferase." J Mol Biol 333:931-949. Pubmed: 14583191
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hjalmarsson, K. J., Bystrom, A. S., Bjork, G. R. (1983). "Purification and characterization of transfer RNA (guanine-1)methyltransferase from Escherichia coli." J Biol Chem 258:1343-1351. Pubmed: 6337136
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837