Ribosomal RNA small subunit methyltransferase A (P06992)

Identification
Name:Ribosomal RNA small subunit methyltransferase A
Synonyms:
  • 16S rRNA dimethyladenosine transferase
  • 16S rRNA dimethylase
  • High level kasugamycin resistance protein ksgA
  • Kasugamycin dimethyltransferase
  • S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase
Gene Name:rsmA
Enzyme Class:
Biological Properties
General Function:Translation, ribosomal structure and biogenesis
Specific Function:Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. Has also a DNA glycosylase/AP lyase activity that removes C mispaired with oxidized T from DNA, and may play a role in protection of DNA against oxidative stress
Cellular Location:Cytoplasm (Potential)
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
GO Classification:
Function
catalytic activity
methyltransferase activity
N-methyltransferase activity
RNA methyltransferase activity
rRNA (adenine) methyltransferase activity
rRNA (adenine-N6,N6-)-dimethyltransferase activity
rRNA methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
rRNA metabolic process
rRNA modification
rRNA processing
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>822 bp
ATGAATAATCGAGTCCACCAGGGCCACTTAGCCCGTAAACGCTTCGGGCAAAACTTTCTC
AACGATCAGTTCGTGATCGACAGTATTGTGTCTGCCATTAACCCGCAAAAGGGCCAGGCG
ATGGTCGAAATCGGCCCCGGTCTGGCGGCATTGACCGAACCGGTCGGCGAACGTCTGGAC
CAGCTGACGGTCATCGAACTTGACCGCGATCTGGCGGCACGTCTGCAAACGCATCCATTC
TTAGGCCCGAAACTGACGATTTATCAGCAGGATGCGATGACCTTTAACTTTGGTGAACTG
GCCGAGAAAATGGGTCAGCCGCTGCGTGTTTTCGGCAACCTGCCTTATAACATCTCCACG
CCGTTGATGTTCCATCTGTTTAGCTATACTGATGCCATTGCCGACATGCACTTTATGTTG
CAAAAAGAGGTGGTGAATCGTCTGGTTGCAGGACCGAACAGCAAAGCGTATGGTCGATTA
AGCGTCATGGCGCAATACTATTGCAATGTGATCCCGGTACTGGAAGTACCGCCGTCAGCC
TTTACACCACCACCCAAAGTGGATTCCGCCGTCGTGCGCCTGGTTCCTCATGCAACGATG
CCTCACCCGGTTAAAGATGTTCGTGTGTTGAGCCGCATCACCACCGAAGCCTTTAACCAG
CGTCGTAAAACCATTCGTAACAGCCTCGGCAACCTGTTTAGCGTCGAGGTGTTAACGGGA
ATGGGGATCGACCCGGCGATGCGAGCGGAAAATATCTCTGTCGCGCAATATTGCCAGATG
GCGAACTATCTGGCGGAGAACGCGCCTTTGCAGGAGAGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:273
Protein Molecular Weight:30420
Protein Theoretical pI:8
PDB File:1QYR
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ribosomal RNA small subunit methyltransferase A
MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEIGPGLAALTEPVGERLD
QLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAEKMGQPLRVFGNLPYNIST
PLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRLSVMAQYYCNVIPVLEVPPSA
FTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAFNQRRKTIRNSLGNLFSVEVLTG
MGIDPAMRAENISVAQYCQMANYLAENAPLQES
References
External Links:
ResourceLink
Uniprot ID:P06992
Uniprot Name:RSMA_ECOLI
GenBank Gene ID:M11054
Genebank Protein ID:146571
PDB ID:1QYR
CCDB:RSMA_ECOLI
General Reference:
  • Blanchin-Roland, S., Blanquet, S., Schmitter, J. M., Fayat, G. (1986). "The gene for Escherichia coli diadenosine tetraphosphatase is located immediately clockwise to folA and forms an operon with ksgA." Mol Gen Genet 205:515-522. Pubmed: 3031429
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Campbell, T. L., Brown, E. D. (2008). "Genetic interaction screens with ordered overexpression and deletion clone sets implicate the Escherichia coli GTPase YjeQ in late ribosome biogenesis." J Bacteriol 190:2537-2545. Pubmed: 18223068
  • Connolly, K., Rife, J. P., Culver, G. (2008). "Mechanistic insight into the ribosome biogenesis functions of the ancient protein KsgA." Mol Microbiol 70:1062-1075. Pubmed: 18990185
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • O'Farrell, H. C., Scarsdale, J. N., Rife, J. P. (2004). "Crystal structure of KsgA, a universally conserved rRNA adenine dimethyltransferase in Escherichia coli." J Mol Biol 339:337-353. Pubmed: 15136037
  • Roa, B. B., Connolly, D. M., Winkler, M. E. (1989). "Overlap between pdxA and ksgA in the complex pdxA-ksgA-apaG-apaH operon of Escherichia coli K-12." J Bacteriol 171:4767-4777. Pubmed: 2670894
  • van Buul, C. P., van Knippenberg, P. H. (1985). "Nucleotide sequence of the ksgA gene of Escherichia coli: comparison of methyltransferases effecting dimethylation of adenosine in ribosomal RNA." Gene 38:65-72. Pubmed: 3905517
  • van Gemen, B., Koets, H. J., Plooy, C. A., Bodlaender, J., Van Knippenberg, P. H. (1987). "Characterization of the ksgA gene of Escherichia coli determining kasugamycin sensitivity." Biochimie 69:841-848. Pubmed: 3122846
  • Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901
  • Zhang-Akiyama, Q. M., Morinaga, H., Kikuchi, M., Yonekura, S., Sugiyama, H., Yamamoto, K., Yonei, S. (2009). "KsgA, a 16S rRNA adenine methyltransferase, has a novel DNA glycosylase/AP lyase activity to prevent mutations in Escherichia coli." Nucleic Acids Res 37:2116-2125. Pubmed: 19223326