ECMDB

Identification

Name:

Thioredoxin-2

Synonyms:

Trx-2
Protein-disulfide reductase

Gene Name:

trxC

Enzyme Class:

1.8.1.8

Biological Properties

General Function:

Involved in electron carrier activity

Specific Function:

Efficient electron donor for the essential enzyme ribonucleotide reductase. Is also able to reduce the interchain disulfide bridges of insulin

Cellular Location:

Cytoplasm

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

KEGG Reactions:

1.0Protein dithiol

1.0

↔

1.0Protein disulfide

1.0

1.0Protein dithiol + 1.0NAD + 1.0NADP ↔ 1.0Protein disulfide + 1.0NADH + 1.0NADPH + 1.0Hydrogen ion
ReactionCard

Complex Reactions:

1.0

1.0Reduced Thioredoxin

→

1.0

1.0Oxidized Thioredoxin

1.0ADP + 1.0Reduced Thioredoxin → 1.0dADP + 1.0Water + 1.0Oxidized Thioredoxin
ReactionCard

1.0

1.0Reduced Thioredoxin

→

1.0

1.0Oxidized Thioredoxin

1.0Guanosine diphosphate + 1.0Reduced Thioredoxin → 1.0dGDP + 1.0Water + 1.0Oxidized Thioredoxin
ReactionCard

1.0

1.0Reduced Thioredoxin

→

1.0

1.0Oxidized Thioredoxin

1.0CDP + 1.0Reduced Thioredoxin → 1.0dCDP + 1.0Water + 1.0Oxidized Thioredoxin
ReactionCard

1.0Reduced Thioredoxin

1.0

→

1.0

1.0Oxidized Thioredoxin

1.0Reduced Thioredoxin + 1.0Uridine 5'-diphosphate → 1.0dUDP + 1.0Water + 1.0Oxidized Thioredoxin
ReactionCard

1.0

1.0Reduced Thioredoxin

→

1.0

1.0Oxidized Thioredoxin

1.0Methionine sulfoxide + 1.0Reduced Thioredoxin → 1.0Water + 1.0L-Methionine + 1.0Oxidized Thioredoxin
ReactionCard

1.0

1.0Reduced Thioredoxin

→

2.0

1.0Oxidized Thioredoxin

1.0Hydrogen peroxide + 1.0Reduced Thioredoxin → 2.0Water + 1.0Oxidized Thioredoxin
ReactionCard

1.0

1.0Reduced Thioredoxin

→

2.0

1.0

1.0Oxidized Thioredoxin

1.0Phosphoadenosine phosphosulfate + 1.0Reduced Thioredoxin → 2.0Hydrogen ion + 1.0Adenosine 3',5'-diphosphate + 1.0Sulfite + 1.0Oxidized Thioredoxin
ReactionCard

1.0

1.0Oxidized Thioredoxin

→

1.0

1.0Reduced Thioredoxin

1.0Hydrogen ion + 1.0NADPH + 1.0Oxidized Thioredoxin → 1.0NADP + 1.0Reduced Thioredoxin
ReactionCard

1.0fused thiol:disulfide interchange protein (oxidized)

1.0Reduced Thioredoxin

→

1.0fused thiol:disulfide interchange protein (reduced)

1.0Oxidized Thioredoxin

1.0fused thiol:disulfide interchange protein (oxidized) + 1.0Reduced Thioredoxin → 1.0fused thiol:disulfide interchange protein (reduced) + 1.0Oxidized Thioredoxin
ReactionCard

1.0Protein dithiol

1.0NAD(P)(+)

→

1.0protein disulfide

1.0NAD(P)H

1.0Protein dithiol + 1.0NAD(P)(+) → 1.0protein disulfide + 1.0NAD(P)H
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00061	Adenosine 3',5'-diphosphate	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB01546	CDP	MetaboCard
ECMDB21326	dADP	MetaboCard
ECMDB01245	dCDP	MetaboCard
ECMDB00960	dGDP	MetaboCard
ECMDB01000	dUDP	MetaboCard
ECMDB01201	Guanosine diphosphate	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB21232	Hydrogen peroxide	MetaboCard
ECMDB00696	L-Methionine	MetaboCard
ECMDB02005	Methionine sulfoxide	MetaboCard
ECMDB00217	NADP	MetaboCard
ECMDB04111	NADPH	MetaboCard
ECMDB01134	Phosphoadenosine phosphosulfate	MetaboCard
ECMDB00240	Sulfite	MetaboCard
ECMDB00295	Uridine 5'-diphosphate	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
disulfide oxidoreductase activity
electron carrier activity
oxidoreductase activity
oxidoreductase activity, acting on a sulfur group of donors
protein disulfide oxidoreductase activity
Process
cell redox homeostasis
cellular homeostasis
cellular process
glycerol ether metabolic process
metabolic process
organic ether metabolic process
small molecule metabolic process

Gene Properties

Blattner:

b2582

Gene Orientation

Clockwise

Centisome Percentage:

58.55

Left Sequence End

2716757

Right Sequence End

2717176

Gene Sequence:

>420 bp
GTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGG
CAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTA
AAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCA
TACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTA
GAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATAC
AAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCAT
AAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGA

Protein Properties

Pfam Domain Function:

Thioredoxin (PF00085 )

Protein Residues:

139

Protein Molecular Weight:

15555

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Thioredoxin-2
MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGETLDKLLKDDLPVVIDFW
APWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRFGIRSIPTIMIFKNGQVVD
MLNGAVPKAPFDSWLNESL

References

External Links:

Resource	Link
Uniprot ID:	P0AGG4
Uniprot Name:	THIO2_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674556
Ecogene ID:	EG11887
Ecocyc:	EG11887
ColiBase:	b2582
Kegg Gene:	b2582
EchoBASE ID:	EB1833
CCDB:	THIO2_ECOLI
BacMap:	16130507

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Miranda-Vizuete, A., Damdimopoulos, A. E., Gustafsson, J., Spyrou, G. (1997). "Cloning, expression, and characterization of a novel Escherichia coli thioredoxin." J Biol Chem 272:30841-30847. Pubmed: 9388228
Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837

Thioredoxin-2 (P0AGG4)