Guanosine diphosphate (ECMDB01201) (M2MDB000296)

General function:

Involved in oxidation-reduction process

Specific function:

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1 contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction of the ribonucleotide. It also provides redox- active cysteines

Gene Name:

nrdA

Uniprot ID:

P00452

Molecular weight:

85774

Reactions

General function:

Involved in RNA binding

Specific function:

Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

Gene Name:

pnp

Uniprot ID:

P05055

Molecular weight:

77100

Reactions

General function:

Involved in bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity

Specific function:

Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP

Gene Name:

apaH

Uniprot ID:

P05637

Molecular weight:

31296

Reactions

General function:

Involved in acid phosphatase activity

Specific function:

A phosphate monoester + H(2)O = an alcohol + phosphate

Gene Name:

appA

Uniprot ID:

P07102

Molecular weight:

47056

Reactions

General function:

Involved in nucleoside diphosphate kinase activity

Specific function:

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate

Gene Name:

ndk

Uniprot ID:

P0A763

Molecular weight:

15463

Reactions

General function:

Involved in adenylosuccinate synthase activity

Specific function:

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

Gene Name:

purA

Uniprot ID:

P0A7D4

Molecular weight:

47345

Reactions

General function:

Involved in ATP binding

Specific function:

ATP + uridine = ADP + UMP

Gene Name:

udk

Uniprot ID:

P0A8F4

Molecular weight:

24353

Reactions

General function:

Involved in magnesium ion binding

Specific function:

ATP + pyruvate = ADP + phosphoenolpyruvate

Gene Name:

pykF

Uniprot ID:

P0AD61

Molecular weight:

50729

Reactions

General function:

Involved in nucleotide binding

Specific function:

ATP-dependent phosphorylation of adenosylcobinamide and adds GMP to adenosylcobinamide phosphate

Gene Name:

cobU

Uniprot ID:

P0AE76

Molecular weight:

20164

Reactions

General function:

Involved in hydrolase activity

Specific function:

Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P), and hydrolysis of thiamine pyrophosphate (TPP) to thiamine monophosphate (TMP). Can hydrolyze other substrates such as MeO-HMP-PP, CF(3)-HMP-PP and MeO-TPP. Is also a non-specific nucleoside tri- and diphosphatase that releases inorganic orthophosphate

Gene Name:

nudJ

Uniprot ID:

P0AEI6

Molecular weight:

17433

General function:

Involved in amino acid binding

Specific function:

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp

Gene Name:

relA

Uniprot ID:

P0AG20

Molecular weight:

83875

Reactions

General function:

Involved in catalytic activity

Specific function:

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the synthesis and degradation of ppGpp. The second messengers ppGpp and c-di-GMP together control biofilm formation in response to translational stress; ppGpp represses biofilm formation while c- di-GMP induces it

Gene Name:

spoT

Uniprot ID:

P0AG24

Molecular weight:

79342

Reactions

General function:

Involved in electron carrier activity

Specific function:

Efficient electron donor for the essential enzyme ribonucleotide reductase. Is also able to reduce the interchain disulfide bridges of insulin

Gene Name:

trxC

Uniprot ID:

P0AGG4

Molecular weight:

15555

Reactions

General function:

Involved in catalytic activity

Specific function:

ATP + sulfate = diphosphate + adenylyl sulfate

Gene Name:

cysD

Uniprot ID:

P21156

Molecular weight:

35188

Reactions

General function:

Involved in magnesium ion binding

Specific function:

ATP + pyruvate = ADP + phosphoenolpyruvate

Gene Name:

pykA

Uniprot ID:

P21599

Molecular weight:

51357

Reactions

General function:

Involved in GTPase activity

Specific function:

May be the GTPase, regulating ATP sulfurylase activity

Gene Name:

cysN

Uniprot ID:

P23845

Molecular weight:

52558

Reactions

General function:

Involved in nucleotidyltransferase activity

Specific function:

Involved in the biosynthesis of the capsular polysaccharide colanic acid

Gene Name:

manC

Uniprot ID:

P24174

Molecular weight:

53016

Reactions

General function:

Involved in catalytic activity

Specific function:

2'-deoxyribonucleoside triphosphate + thioredoxin disulfide + H(2)O = ribonucleoside triphosphate + thioredoxin

Gene Name:

nrdD

Uniprot ID:

P28903

Molecular weight:

80022

Reactions

General function:

Involved in hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides

Specific function:

Could participate in the regulation of cell wall biosynthesis by influencing the concentration of GDP-mannose or GDP-glucose in the cell. May be involved in the degradation of GDP-mannose and GDP-glucose, diverting the GDP to the synthesis of GDP-fucose as required. Might also be involved in the biosynthesis of the slime polysaccharide colanic acid

Gene Name:

nudD

Uniprot ID:

P32056

Molecular weight:

18273

Reactions

General function:

Involved in oxidoreductase activity

Specific function:

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2F contains the tyrosyl radical required for catalysis

Gene Name:

nrdF

Uniprot ID:

P37146

Molecular weight:

36443

Reactions

General function:

Involved in GTPase activity

Specific function:

May play a role in 30S ribosomal subunit biogenesis. Unusual circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover. Dispensible for viability, but important for overall fitness. The intrinsic GTPase activity is stimulated by the presence of 30S (160-fold increase in kcat) or 70S (96 fold increase in kcat) ribosomes (PubMed:14973029). The GTPase is inhibited by aminoglycoside antibiotics such as neomycin and paromycin (PubMed:15466596) streptomycin and spectinomycin (PubMed:15828870). This inhibition is not due to competition for binding sites on the 30S or 70S ribosome (PubMed:15828870)

Gene Name:

rsgA

Uniprot ID:

P39286

Molecular weight:

39193

General function:

Involved in oxidation-reduction process

Specific function:

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1E contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction of the ribonucleotide

Gene Name:

nrdE

Uniprot ID:

P39452

Molecular weight:

80478

Reactions

General function:

Involved in protein binding

Specific function:

Essential for recycling GMP and indirectly, cGMP

Gene Name:

gmk

Uniprot ID:

P60546

Molecular weight:

23593

Reactions

General function:

Involved in ATP binding

Specific function:

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in the energy metabolism and nucleotide synthesis, is essential for maintenance and cell growth

Gene Name:

adk

Uniprot ID:

P69441

Molecular weight:

23586

Reactions

General function:

Involved in oxidoreductase activity

Specific function:

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis

Gene Name:

nrdB

Uniprot ID:

P69924

Molecular weight:

43517

Reactions

General function:

Involved in electron carrier activity

Specific function:

Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters (Probable)

Gene Name:

grxD

Uniprot ID:

P0AC69

Molecular weight:

12879

General function:

Involved in electron carrier activity

Specific function:

The disulfide bond functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. In addition, it is also involved in reducing some disulfides in a coupled system with glutathione reductase

Gene Name:

grxC

Uniprot ID:

P0AC62

Molecular weight:

9137

General function:

Involved in protein binding

Specific function:

Involved in reducing some disulfides in a coupled system with glutathione reductase. Does not act as hydrogen donor for ribonucleotide reductase

Gene Name:

grxB

Uniprot ID:

P0AC59

Molecular weight:

24350

General function:

Involved in electron carrier activity

Specific function:

The disulfide bond functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. In addition, it is also involved in reducing some disulfides in a coupled system with glutathione reductase

Gene Name:

grxA

Uniprot ID:

P68688

Molecular weight:

9685

General function:

Involved in electron carrier activity

Specific function:

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions

Gene Name:

trxA

Uniprot ID:

P0AA25

Molecular weight:

11807