Phospholipase A1 (P0A921)

• Detergent-resistant phospholipase A
• DR-phospholipase A
• Outer membrane phospholipase A
• OM PLA
• OMPLA
• Phosphatidylcholine 1-acylhydrolase

• 3.1.1.32
• 3.1.1.4

• Glycerophospholipid metabolism ec00564
• Metabolic pathways eco01100
• alpha-Linolenic acid metabolism ec00592

>870 bp
ATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAG
GGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACC
CCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCC
GTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCGTTCTGCAAAGAC
TCCATTCTGGAAGCCATCGACGCAGGCATCAAACTGATTATCACCATCACTGAAGGCATC
CCGACGCTGGATATGCTGACCGTGAAAGTGAAGCTGGATGAAGCAGGCGTTCGTATGATC
GGCCCGAACTGCCCAGGCGTTATCACTCCGGGTGAATGCAAAATCGGTATCCAGCCTGGT
CACATTCACAAACCGGGTAAAGTGGGTATCGTTTCCCGTTCCGGTACACTGACCTATGAA
GCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGT
GACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAG
ACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAGCGCTGAAGAAGAAGCAGCTGCG
TACATCAAAGAGCACGTTACCAAGCCAGTTGTGGGTTACATCGCTGGTGTGACTGCGCCG
AAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGAT
GAGAAATTCGCTGCTCTGGAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATC
GGTGAAGCACTGAAAACTGTTCTGAAATAA

• PLA1 (PF02253 )

• 1-20

• 53-65
• 85-99
• 106-118
• 129-148
• 151-164
• 174-186
• 189-198
• 217-223
• 226-234
• 242-250
• 256-265
• 275-286

>Phospholipase A1
MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQEHDNPFTLYPYDTNYL
IYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGILGPNSVLGASYTQKSWWQL
SNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGYNHDSNGRSDPTSRSWNRLYT
RLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLKIGYHLGDAVLSAKGQYNWNTGY
GGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQTRVGVGVMLNDLF

• Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
• Brok, R. G., Brinkman, E., van Boxtel, R., Bekkers, A. C., Verheij, H. M., Tommassen, J. (1994). "Molecular characterization of enterobacterial pldA genes encoding outer membrane phospholipase A." J Bacteriol 176:861-870. Pubmed: 8300539
• Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
• de Geus, P., Verheij, H. M., Riegman, N. H., Hoekstra, W. P., de Haas, G. H. (1984). "The pro- and mature forms of the E. coli K-12 outer membrane phospholipase A are identical." EMBO J 3:1799-1802. Pubmed: 6383820
• Dekker, N., Merck, K., Tommassen, J., Verheij, H. M. (1995). "In vitro folding of Escherichia coli outer-membrane phospholipase A." Eur J Biochem 232:214-219. Pubmed: 7556153
• Dekker, N., Tommassen, J., Lustig, A., Rosenbusch, J. P., Verheij, H. M. (1997). "Dimerization regulates the enzymatic activity of Escherichia coli outer membrane phospholipase A." J Biol Chem 272:3179-3184. Pubmed: 9013551
• Dekker, N., Tommassen, J., Verheij, H. M. (1999). "Bacteriocin release protein triggers dimerization of outer membrane phospholipase A in vivo." J Bacteriol 181:3281-3283. Pubmed: 10322034
• Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
• Homma, H., Chiba, N., Kobayashi, T., Kudo, I., Inoue, K., Ikeda, H., Sekiguchi, M., Nojima, S. (1984). "Characteristics of detergent-resistant phospholipase A overproduced in E. coli cells bearing its cloned structural gene." J Biochem 96:1645-1653. Pubmed: 6397463
• Homma, H., Kobayashi, T., Chiba, N., Karasawa, K., Mizushima, H., Kudo, I., Inoue, K., Ikeda, H., Sekiguchi, M., Nojima, S. (1984). "The DNA sequence encoding pldA gene, the structural gene for detergent-resistant phospholipase A of E. coli." J Biochem 96:1655-1664. Pubmed: 6397464
• Horrevoets, A. J., Verheij, H. M., de Haas, G. H. (1991). "Inactivation of Escherichia coli outer-membrane phospholipase A by the affinity label hexadecanesulfonyl fluoride. Evidence for an active-site serine." Eur J Biochem 198:247-253. Pubmed: 2040286
• Irino, N., Nakayama, K., Nakayama, H. (1986). "The recQ gene of Escherichia coli K12: primary structure and evidence for SOS regulation." Mol Gen Genet 205:298-304. Pubmed: 3027506
• Snijder, H. J., Kingma, R. L., Kalk, K. H., Dekker, N., Egmond, M. R., Dijkstra, B. W. (2001). "Structural investigations of calcium binding and its role in activity and activation of outer membrane phospholipase A from Escherichia coli." J Mol Biol 309:477-489. Pubmed: 11371166
• Snijder, H. J., Ubarretxena-Belandia, I., Blaauw, M., Kalk, K. H., Verheij, H. M., Egmond, M. R., Dekker, N., Dijkstra, B. W. (1999). "Structural evidence for dimerization-regulated activation of an integral membrane phospholipase." Nature 401:717-721. Pubmed: 10537112
• Snijder, H. J., Van Eerde, J. H., Kingma, R. L., Kalk, K. H., Dekker, N., Egmond, M. R., Dijkstra, B. W. (2001). "Structural investigations of the active-site mutant Asn156Ala of outer membrane phospholipase A: function of the Asn-His interaction in the catalytic triad." Protein Sci 10:1962-1969. Pubmed: 11567087