ECMDB: Vitamin B12-binding protein (P37028)

Identification

Name:

Vitamin B12-binding protein

Synonyms:

Not Available

Gene Name:

btuF

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in binding

Specific Function:

Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC

Cellular Location:

Periplasm

SMPDB Pathways:

adenosylcobalamin salvage from cobinamide PW001884

KEGG Pathways:

ABC transporters ec02010

SMPDB Reactions:

1.0Cobinamide

1.0

→

1.0Adenosine diphosphate

1.0

1.0Cobinamide

1.0

1.0Cobinamide + 1.0Adenosine triphosphate + 1.0Water + 1.0Cobinamide → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0Cobinamide + 1.0ADP
ReactionCard

1.0Cyanocobalamin

1.0

→

1.0Adenosine diphosphate

1.0

1.0Cyanocobalamin

1.0

1.0Cyanocobalamin + 1.0Adenosine triphosphate + 1.0Water + 1.0Cyanocobalamin → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0Cyanocobalamin + 1.0ADP
ReactionCard

EcoCyc Reactions:

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Water + 1.0Cob(I)alamin → 1.0ADP + 1.0Cob(I)alamin + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Water + 1.0Adenosylcobalamin → 1.0Adenosylcobalamin + 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Water + 1.0Cobinamide → 1.0ADP + 1.0Cobinamide + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB02086	Adenosylcobalamin	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB04040	Cob(I)alamin	MetaboCard
ECMDB06902	Cobinamide	MetaboCard
ECMDB21386	Cyanocobalamin	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB01429	Phosphate	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
cation transmembrane transporter activity
di-, tri-valent inorganic cation transmembrane transporter activity
inorganic cation transmembrane transporter activity
ion transmembrane transporter activity
iron ion transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
cation transport
establishment of localization
high-affinity iron ion transport
ion transport
iron ion transmembrane transport
iron ion transport
metal ion transport
transition metal ion transport
transport

Gene Properties

Blattner:

b0158

Gene Orientation

Counterclockwise

Centisome Percentage:

3.83

Left Sequence End

177662

Right Sequence End

178462

Gene Sequence:

>801 bp
ATGGCTAAGTCACTGTTCAGGGCGCTGGTCGCCCTGTCTTTTCTTGCGCCACTGTGGCTC
AACGCCGCGCCGCGCGTCATCACGCTTTCTCCCGCCAACACTGAACTTGCCTTTGCCGCC
GGGATCACGCCGGTTGGGGTCAGCAGCTATTCCGACTATCCTCCACAAGCGCAAAAGATT
GAGCAGGTTTCCACCTGGCAGGGGATGAATCTGGAACGCATTGTCGCGCTGAAACCCGAT
CTGGTGATTGCCTGGCGTGGAGGTAATGCCGAGCGGCAGGTTGACCAGCTGGCTTCGCTG
GGAATAAAAGTGATGTGGGTCGATGCGACAAGCATTGAACAAATTGCCAATGCGTTACGT
CAACTGGCCCCCTGGAGTCCGCAACCAGACAAGGCCGAACAAGCCGCGCAATCCCTGCTG
GATCAGTACGCGCAATTGAAAGCGCAATATGCTGATAAACCTAAAAAACGTGTTTTTCTG
CAATTCGGCATTAATCCGCCATTTACCAGTGGAAAAGAGTCGATTCAGAACCAGGTACTC
GAAGTTTGTGGCGGAGAAAACATCTTTAAAGACAGCCGGGTTCCCTGGCCGCAAGTTAGC
CGCGAACAGGTGTTAGCACGCTCGCCACAGGCGATTGTCATTACAGGCGGACCGGACCAA
ATTCCTAAAATCAAACAATACTGGGGTGAACAGCTCAAAATTCCCGTTATTCCTCTCACG
AGTGACTGGTTTGAACGTGCAAGCCCACGTATTATCCTCGCTGCACAACAGCTCTGTAAT
GCGCTTTCACAGGTAGATTAG

Protein Properties

Pfam Domain Function:

Peripla_BP_2 (PF01497 )

Protein Residues:

266

Protein Molecular Weight:

29367

Protein Theoretical pI:

PDB File:

1N2Z

Signaling Regions:

1-22

Transmembrane Regions:

None

Protein Sequence:

>Vitamin B12-binding protein
MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITPVGVSSYSDYPPQAQKI
EQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGIKVMWVDATSIEQIANALR
QLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFLQFGINPPFTSGKESIQNQVL
EVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGPDQIPKIKQYWGEQLKIPVIPLT
SDWFERASPRIILAAQQLCNALSQVD

References

External Links:

Resource	Link
Uniprot ID:	P37028
Uniprot Name:	BTUF_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674362
PDB ID:	1N2Z
Ecogene ID:	EG12334
Ecocyc:	EG12334
ColiBase:	b0158
Kegg Gene:	b0158
EchoBASE ID:	EB2238
CCDB:	BTUF_ECOLI
BacMap:	16128151

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Borths, E. L., Locher, K. P., Lee, A. T., Rees, D. C. (2002). "The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter." Proc Natl Acad Sci U S A 99:16642-16647. Pubmed: 12475936
Cadieux, N., Bradbeer, C., Reeger-Schneider, E., Koster, W., Mohanty, A. K., Wiener, M. C., Kadner, R. J. (2002). "Identification of the periplasmic cobalamin-binding protein BtuF of Escherichia coli." J Bacteriol 184:706-717. Pubmed: 11790740
Fujita, N., Mori, H., Yura, T., Ishihama, A. (1994). "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Nucleic Acids Res 22:1637-1639. Pubmed: 8202364
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Karpowich, N. K., Huang, H. H., Smith, P. C., Hunt, J. F. (2003). "Crystal structures of the BtuF periplasmic-binding protein for vitamin B12 suggest a functionally important reduction in protein mobility upon ligand binding." J Biol Chem 278:8429-8434. Pubmed: 12468528