| Identification |
|---|
| Name: | Aminomethyltransferase |
|---|
| Synonyms: | - Glycine cleavage system T protein
|
|---|
| Gene Name: | gcvT |
|---|
| Enzyme Class: | |
|---|
| Biological Properties |
|---|
| General Function: | Involved in aminomethyltransferase activity |
|---|
| Specific Function: | The glycine cleavage system catalyzes the degradation of glycine |
|---|
| Cellular Location: | Not Available |
|---|
| SMPDB Pathways: | |
|---|
| KEGG Pathways: | |
|---|
| KEGG Reactions: | | | |
1.0 | + | 1.0 | + | 1.0 | ↔ | 1.0 | + | 1.0 | + | 1.0 | + | 1.0 | + | 1.0 |
| | | | |
1.0S-Aminomethyldihydrolipoylprotein | + | 1.0 | + | 1.0S-Aminomethyldihydrolipoylprotein | ↔ | 1.0Dihydrolipoylprotein | + | 1.0 | + | 1.0 |
| | |
1.0 | + | 1.0 | + | 1.0 | → | 1.0 | + | 1.0 | + | 1.0 | + | 1.0 | + | 1.0 |
| |
|
|---|
| SMPDB Reactions: | |
1.0S-Aminomethyldihydrolipoylprotein; | + | 1.0Tetrahydrofolic acid | + | 1.0 | ↔ | 1.05,10-Methylene-THF | + | 1.0 | + | 1.0dihydrolipoylprotein | + | 1.0 |
| | |
1.0 | + | 1.0 | → | 1.0N5-Formyl-THF | + | 1.0 |
| |
|
|---|
| EcoCyc Reactions: | | | |
1.0 | + | 1.0 | + | 1.0 | → | 1.0 | + | 1.0 | + | 1.0 | + | 1.0 | + | 1.0 |
| |
|
|---|
| Complex Reactions: | |
1.0 | + | 1.0 | + | 1.0 | → | 1.0 | + | 1.0 | + | 1.0 | + | 1.0 |
| | |
1.0[Protein]-S(8)-aminomethyldihydrolipoyllysine | + | 1.0 | → | 1.0[protein]-dihydrolipoyllysine | + | 1.0 | + | 1.0 |
| |
|
|---|
| Metabolites: | |
|---|
| GO Classification: | | Component |
|---|
| cell part | | cytoplasm | | intracellular part | | Function |
|---|
| aminomethyltransferase activity | | catalytic activity | | methyltransferase activity | | transferase activity | | transferase activity, transferring one-carbon groups | | Process |
|---|
| cellular amino acid and derivative metabolic process | | cellular amino acid metabolic process | | cellular metabolic process | | glycine catabolic process | | glycine metabolic process | | metabolic process | | serine family amino acid metabolic process |
|
|---|
| Gene Properties |
|---|
| Blattner: | b2905 |
|---|
| Gene Orientation | Counterclockwise |
|---|
| Centisome Percentage: | 65.69 |
|---|
| Left Sequence End | 3047595 |
|---|
| Right Sequence End | 3048689 |
|---|
| Gene Sequence: | >1095 bp
ATGTCGCGCGTCAATCCTCTATCTTCGCTGTCATTGTTGGCGGTTCTGGTTTTAGCGGGG
TGTAGTAGTCAGGCTCCGCAACCGTTGAAAAAGGGCGAAAAAGCGATAGATGTAGCGAGC
GTCGTGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTC
GCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTG
GCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAG
ATCGCCTGGCAAGAGATTGACCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTT
CATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCG
GTGCGTACAGAAAAGCAATTGAGCGCGATTTTTGACGATCTGATCAACATGGTGCCAATG
GGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGC
ATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGAAAATGGACGGTACAGTCCGT
CAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTAT
CCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCC
AGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTG
GACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGC
AAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGT
GACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACG
AAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAA
ATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGT
TGTATGAAACAGTGA |
|---|
| Protein Properties |
|---|
| Pfam Domain Function: | |
|---|
| Protein Residues: | 364 |
|---|
| Protein Molecular Weight: | 40146 |
|---|
| Protein Theoretical pI: | 5 |
|---|
| PDB File: | 1VLO |
| Signaling Regions: | |
|---|
| Transmembrane Regions: | |
|---|
| Protein Sequence: | >Aminomethyltransferase
MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDAGMFDVSHMTIVDLRGS
RTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVYYFTEDFFRLVVNSATREK
DLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLFNDAQRQAVEGMKPFFGVQAG
DLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKPCGLGARDTLRLEAGMNLYGQEM
DETISPLAANMGWTIAWEPADRDFIGREALEVQREHGTEKLVGLVMTEKGVLRNELPVRF
TDAQGNQHEGIITSGTFSPTLGYSIALARVPEGIGETAIVQIRNREMPVKVTKPVFVRNG
KAVA |
|---|
| References |
|---|
| External Links: | |
|---|
| General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
- Okamura-Ikeda, K., Ohmura, Y., Fujiwara, K., Motokawa, Y. (1993). "Cloning and nucleotide sequence of the gcv operon encoding the Escherichia coli glycine-cleavage system." Eur J Biochem 216:539-548. Pubmed: 8375392
- Stauffer, L. T., Ghrist, A., Stauffer, G. V. (1993). "The Escherichia coli gcvT gene encoding the T-protein of the glycine cleavage enzyme system." DNA Seq 3:339-346. Pubmed: 8219277
|
|---|
