Vitamin B12 import ATP-binding protein BtuD (P06611)

Identification
Name:Vitamin B12 import ATP-binding protein BtuD
Synonyms:
  • Vitamin B12-transporting ATPase
Gene Name:btuD
Enzyme Class:
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Responsible for energy coupling to the transport system
Cellular Location:Cell inner membrane; Peripheral membrane protein
SMPDB Pathways:
  • adenosylcobalamin salvage from cobinamide PW001884
KEGG Pathways:
SMPDB Reactions:
1.0Cobinamide+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Cobinamide+1.0Thumb
1.0Cobinamide + 1.0Adenosine triphosphate + 1.0Water + 1.0Cobinamide → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0Cobinamide + 1.0ADP
ReactionCard
1.0Cyanocobalamin+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Cyanocobalamin+1.0Thumb
1.0Cyanocobalamin + 1.0Adenosine triphosphate + 1.0Water + 1.0Cyanocobalamin → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0Cyanocobalamin + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Cob(I)alamin → 1.0ADP + 1.0Cob(I)alamin + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Adenosylcobalamin → 1.0Adenosylcobalamin + 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Cobinamide → 1.0ADP + 1.0Cobinamide + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0vitamin B12(Out) → 1.0Thumb+1.0Thumb+1.0vitamin B12(In)
1.0Adenosine triphosphate + 1.0Water + 1.0vitamin B12(Out) → 1.0ADP + 1.0Inorganic phosphate + 1.0vitamin B12(In)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB02086AdenosylcobalaminMetaboCard
ECMDB01341ADPMetaboCard
ECMDB04040Cob(I)alaminMetaboCard
ECMDB06902CobinamideMetaboCard
ECMDB21386CyanocobalaminMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ATPase activity
binding
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
purine nucleoside binding
pyrophosphatase activity
Gene Properties
Blattner:b1709
Gene OrientationCounterclockwise
Centisome Percentage:38.60
Left Sequence End1790833
Right Sequence End1791582
Gene Sequence:
>750 bp
ATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCG
TTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAG
CGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCC
AGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAG
CAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCGCTCAAGGCGAGTGACAGACGCTGG
CAGGCGCTGGCGGAAAACAACCACTTTTTAAGCAGCGACCATCTGCATAACATTACCGAA
GTGGCGATTCACCGCCTGGAGCAGCAGCTTGGCAAGCCTTACGTCTGGGGCGGTACGCGG
CCTGATAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAG
GCTAAGCTCCCGCGCACGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCG
AACAACGACCTGCGCCGGGGAGATTTGCTGTTTTTCCATATCCACAGCCGCGAGATAGCC
GATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAA
ACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGC
AGGATTTTGACGGAAGAGACGATTTTGTAG
Protein Properties
Pfam Domain Function:
Protein Residues:249
Protein Molecular Weight:27081
Protein Theoretical pI:10
PDB File:1L7V
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Vitamin B12 import ATP-binding protein BtuD
MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLARMAGMTSGKGSIQFAG
QPLEAWSATKLALHRAYLSQQQTPPFATPVWHYLTLHQHDKTRTELLNDVAGALALDDKL
GRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEPMNSLDVAQQSALDKILSALC
QQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRREEVLTPPNLAQAYGMNFRRLDIE
GHRMLISTI
References
External Links:
ResourceLink
Uniprot ID:P06611
Uniprot Name:BTUD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674388
PDB ID:1L7V
Ecogene ID:EG10128
Ecocyc:EG10128
ColiBase:b1709
Kegg Gene:b1709
EchoBASE ID:EB0126
CCDB:BTUD_ECOLI
BacMap:16129665
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Friedrich, M. J., de Veaux, L. C., Kadner, R. J. (1986). "Nucleotide sequence of the btuCED genes involved in vitamin B12 transport in Escherichia coli and homology with components of periplasmic-binding-protein-dependent transport systems." J Bacteriol 167:928-934. Pubmed: 3528129
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Locher, K. P., Lee, A. T., Rees, D. C. (2002). "The E. coli BtuCD structure: a framework for ABC transporter architecture and mechanism." Science 296:1091-1098. Pubmed: 12004122