Displaying growth conditions 801 - 825 of 1187 in total
ECMDB ID NameConcentrationStrain
Media
Growth Status
Growth System
TemperatureCitation
ECMDB03357N-Acetylornithine43± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB03357N-Acetylornithine398± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB03357N-Acetylornithine152± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L acetate
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB03357N-Acetylornithine14± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB03357N-Acetylornithine0.61± 0.03 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
Download Details
ECMDB03335IDP75± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB03335IDP24± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB03070Shikimic acid14± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB02908Sodium188000± 3000 uMK12
16 mM NaH2PO4; 32 mM Na2HPO4; 5 mM (NH4)2SO4; 40 mM NaCl; 5 mM KCl; 0.4 mM MgSO4, 55 mM glucose
Stationary Phase (50 mM chloride in media)
Shake flask
37 oCPMID: 13909522
ECMDB02908Sodium70000± 2000 uMK12
16 mM NaH2PO4; 32 mM Na2HPO4; 5 mM (NH4)2SO4; 40 mM NaCl; 5 mM KCl; 0.4 mM MgSO4, 55 mM glucose
Mid-Log Phase (50 mM chloride in media)
Shake flask
37 oCPMID: 13909522
ECMDB02908Sodium5000± 0 uMK-12
Not Available
Not Available
Not Available
Not Available1. Cybercell Database: http://ccdb.wishartlab.com/CCDB/cgi-bin/STAT_NEW.cgi
2. Phillips R., Kondev, J., Theriot, J. (2008) “Physical Biology of the Cell” Garland Science, New York, NY.
ECMDB02654Phenylethylamine0.43± 0.11 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
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ECMDB02649D-Erythrose 4-phosphate1± 0 uMW3110
4.0 g/L Na2SO4; 5.36 g/L (NH4)2SO4; 1.0 g/L NH4Cl; 7.3 g/L K2HPO4; 1.8 g/L NaH2PO4 H2O; 12.0 g/L (NH4)2-H-citrate; 4.0 mL/L MgSO4 (1 M); 6.0 mL/L trace element solution; 0.02 g/L thiamine, 20 g/L glucose
Mid Log Phase
Bioreactor, pH controlled, aerated, dilution rate=0.125 L/h
37 oCPark, C., Park, C., Lee, Y., Lee, S.Y., Oh, H.B., Lee, J. (2011) Determination of the Intracellular Concentration of Metabolites in Escherichia coli Collected during the Exponential and Stationary Growth Phases using Liquid Chromatography-Mass Spectrometry. Bull Korean Chem. Soc. 32: 524-530.
ECMDB02649D-Erythrose 4-phosphate3± 0 uMW3110
4.0 g/L Na2SO4; 5.36 g/L (NH4)2SO4; 1.0 g/L NH4Cl; 7.3 g/L K2HPO4; 1.8 g/L NaH2PO4 H2O; 12.0 g/L (NH4)2-H-citrate; 4.0 mL/L MgSO4 (1 M); 6.0 mL/L trace element solution; 0.02 g/L thiamine, 20 g/L glucose
Stationary Phase
Bioreactor, pH controlled, aerated
37 oCPark, C., Park, C., Lee, Y., Lee, S.Y., Oh, H.B., Lee, J. (2011) Determination of the Intracellular Concentration of Metabolites in Escherichia coli Collected during the Exponential and Stationary Growth Phases using Liquid Chromatography-Mass Spectrometry. Bull Korean Chem. Soc. 32: 524-530.
ECMDB02322Cadaverine1750± 0 uMK12 HB101
199 Medium with Earle’s salts –which contains 21 amino acids, 17 vitamins, 10 components of nucleic acids, sodium acetate, glucose, NaC1, KCl, CaC12, MgS04, Na2HP04, and Fe(N03)3
Mid Log Phase
Shake flask
37 oCPMID: 8742354
ECMDB02322Cadaverine200± 0 uMK12 HB101
199 Medium with Earle’s salts –which contains 21 amino acids, 17 vitamins, 10 components of nucleic acids, sodium acetate, glucose, NaC1, KCl, CaC12, MgS04, Na2HP04, and Fe(N03)3
Stationary Phase
Shake flask
37 oCPMID: 8742354
ECMDB02251L-Homoserine16± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB02033D-Ribose-5-phosphate1020± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB02033D-Ribose-5-phosphate686± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L acetate
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB02033D-Ribose-5-phosphate1320± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB02005Methionine sulfoxide7± 1 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
Download Details
ECMDB01586Glucose 1-phosphate413± 0 uMW3110
4.0 g/L Na2SO4; 5.36 g/L (NH4)2SO4; 1.0 g/L NH4Cl; 7.3 g/L K2HPO4; 1.8 g/L NaH2PO4 H2O; 12.0 g/L (NH4)2-H-citrate; 4.0 mL/L MgSO4 (1 M); 6.0 mL/L trace element solution; 0.02 g/L thiamine, 20 g/L glucose
Mid Log Phase
Bioreactor, pH controlled, aerated, dilution rate=0.125 L/h
37 oCPark, C., Park, C., Lee, Y., Lee, S.Y., Oh, H.B., Lee, J. (2011) Determination of the Intracellular Concentration of Metabolites in Escherichia coli Collected during the Exponential and Stationary Growth Phases using Liquid Chromatography-Mass Spectrometry. Bull Korean Chem. Soc. 32: 524-530.
ECMDB01586Glucose 1-phosphate89± 0 uMW3110
4.0 g/L Na2SO4; 5.36 g/L (NH4)2SO4; 1.0 g/L NH4Cl; 7.3 g/L K2HPO4; 1.8 g/L NaH2PO4 H2O; 12.0 g/L (NH4)2-H-citrate; 4.0 mL/L MgSO4 (1 M); 6.0 mL/L trace element solution; 0.02 g/L thiamine, 20 g/L glucose
Stationary Phase
Bioreactor, pH controlled, aerated
37 oCPark, C., Park, C., Lee, Y., Lee, S.Y., Oh, H.B., Lee, J. (2011) Determination of the Intracellular Concentration of Metabolites in Escherichia coli Collected during the Exponential and Stationary Growth Phases using Liquid Chromatography-Mass Spectrometry. Bull Korean Chem. Soc. 32: 524-530.
ECMDB01586Glucose 1-phosphate33± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB01546CDP144± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
Displaying growth conditions 801 - 825 of 1187 in total