Putative monooxygenase rutA (P75898)

Identification
Name:Putative monooxygenase rutA
Synonyms:
  • Pyrimidine utilization protein A
Gene Name:rutA
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Specific Function:Part of the rut operon, which is required for the utilization of pyrimidines as sole nitrogen source
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Uracil + 1.0FMNH + 1.0Oxygen ↔ 1.0Ureidoacrylate peracid + 1.0Flavin Mononucleotide
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Uracil + 1.0FMNH + 1.0Oxygen + 1.0Thymine ↔ 1.0Ureidoacrylate peracid + 1.0Flavin Mononucleotide + 1.0(Z)-2-Methyl-ureidoacrylate peracid
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Uracil + 1.0FMNH2 + 1.0Oxygen → 1.0Ureidoacrylate peracid + 1.0Flavin Mononucleotide + 1.0Hydrogen ion + 1.0Peroxyaminoacrylate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Uracil + 1.0FMNH + 1.0Oxygen ↔ 1.0Ureidoacrylate peracid + 1.0Flavin Mononucleotide
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0(Z)-2-methylureidoacrylate peracid+1.0Thumb+1.0Thumb
1.0Thymine + 1.0Oxygen + 1.0FMNH → 1.0(Z)-2-methylureidoacrylate peracid + 1.0Flavin Mononucleotide + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Uracil + 1.0Oxygen + 1.0FMNH → 1.0Hydrogen ion + 1.0Ureidoacrylate peracid + 1.0Flavin Mononucleotide
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0NADH + 1.0Oxygen + 1.0Uracil → 1.0NAD + 1.0Ureidoacrylate peracid
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Uracil + 1.0FMNH(2) + 1.0Oxygen → 1.0Ureidoacrylate peracid + 1.0Flavin Mononucleotide + 1.0Water
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Thymine + 1.0FMNH(2) + 1.0Oxygen → 1.0(Z)-2-Methyl-ureidoacrylate peracid + 1.0Flavin Mononucleotide + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB23105(Z)-2-Methyl-ureidoacrylate peracidMetaboCard
ECMDB01520Flavin MononucleotideMetaboCard
ECMDB01142FMNHMetaboCard
ECMDB23187FMNH(2)MetaboCard
ECMDB24059FMNH2MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB04124OxygenMetaboCard
ECMDB21275PeroxyaminoacrylateMetaboCard
ECMDB00262ThymineMetaboCard
ECMDB00300UracilMetaboCard
ECMDB21299Ureidoacrylate peracidMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b1012
Gene OrientationCounterclockwise
Centisome Percentage:23.11
Left Sequence End1072086
Right Sequence End1073234
Gene Sequence:
>1149 bp
TTGATTAAGTCAGCGCTATTGGTTCTGGAAGACGGAACCCAGTTTCACGGTCGGGCCATA
GGGGCAACAGGTTCGGCGGTTGGGGAAGTCGTTTTCAATACTTCAATGACCGGTTATCAA
GAAATCCTCACTGATCCTTCCTATTCTCGTCAAATCGTTACTCTTACTTATCCCCATATT
GGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTG
GTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCT
TACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGACGCGT
TTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCG
GCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAA
GAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGC
CTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGT
GCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCG
GCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAAC
GGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAA
ACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGT
GCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTG
GAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACA
TTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATT
CATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCA
CACGACGCCGCGCCGTTGTTCGACCACTTTATCGAGTTAATTGAGCAGTACCGTAAAACC
GCTAAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:382
Protein Molecular Weight:42219
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Putative monooxygenase rutA
MQDAAPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYH
FDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMA
ATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKSD
FKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFAPTA
ARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRS
GTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIETF
GERIQPLMQCRAHLPALTQEVA
References
External Links:
ResourceLink
Uniprot ID:P75898
Uniprot Name:RUTA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321914
Ecogene ID:EG13859
Ecocyc:EG13859
ColiBase:b1012
Kegg Gene:b1012
EchoBASE ID:EB3619
CCDB:RUTA_ECOLI
BacMap:49176071
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Loh, K. D., Gyaneshwar, P., Markenscoff Papadimitriou, E., Fong, R., Kim, K. S., Parales, R., Zhou, Z., Inwood, W., Kustu, S. (2006). "A previously undescribed pathway for pyrimidine catabolism." Proc Natl Acad Sci U S A 103:5114-5119. Pubmed: 16540542
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232