Acyl carrier protein phosphodiesterase (P21515)

Identification
Name:Acyl carrier protein phosphodiesterase
Synonyms:
  • ACP phosphodiesterase
Gene Name:acpH
Enzyme Class:
Biological Properties
General Function:Involved in [acyl-carrier-protein] phosphodiesterase activity
Specific Function:Converts holo-ACP to apo-ACP by hydrolytic cleavage of the phosphopantetheine prosthetic group from ACP
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
  • Pantothenate and CoA biosynthesis ec00770
KEGG Reactions:
1.0Acyl-carrier protein+1.0Thumb+1.0Acyl-carrier protein ↔ 1.0Thumb+1.0Apo-[acyl-carrier-protein]
1.0Acyl-carrier protein + 1.0Water + 1.0Acyl-carrier protein ↔ 1.0Pantetheine 4'-phosphate + 1.0Apo-[acyl-carrier-protein]
ReactionCard
Complex Reactions:
1.0Decanoyl-ACP (n-C10:0ACP)+1.0Thumb → 1.0acyl carrier protein+1.0Thumb+1.0Thumb
1.0Decanoyl-ACP (n-C10:0ACP) + 1.0Water → 1.0acyl carrier protein + 1.0Decanoate (N-C10:0) + 1.0Hydrogen ion
ReactionCard
1.0Dodecanoyl-ACP (n-C12:0ACP)+1.0Thumb → 1.0acyl carrier protein+1.0Thumb+1.0Thumb
1.0Dodecanoyl-ACP (n-C12:0ACP) + 1.0Water → 1.0acyl carrier protein + 1.0Dodecanoate (N-C12:0) + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0cis-tetradec-7-enoyl-[acyl-carrier protein] (n-C14:1) → 1.0acyl carrier protein+1.0Thumb+1.0Thumb
1.0Water + 1.0cis-tetradec-7-enoyl-[acyl-carrier protein] (n-C14:1) → 1.0acyl carrier protein + 1.0Hydrogen ion + 1.0Tetradecenoate (N-C14:1)
ReactionCard
1.0Thumb+1.0Myristoyl-ACP (n-C14:0ACP) → 1.0acyl carrier protein+1.0Thumb+1.0Thumb
1.0Water + 1.0Myristoyl-ACP (n-C14:0ACP) → 1.0acyl carrier protein + 1.0Hydrogen ion + 1.0tetradecanoate (n-C14:0)
ReactionCard
1.0Thumb+1.0Octanoyl-ACP (n-C8:0ACP) → 1.0acyl carrier protein+1.0Thumb+1.0Thumb
1.0Water + 1.0Octanoyl-ACP (n-C8:0ACP) → 1.0acyl carrier protein + 1.0Hydrogen ion + 1.0Caprylic acid
ReactionCard
1.0Thumb+1.0Palmitoyl-ACP (n-C16:0ACP) → 1.0acyl carrier protein+1.0Thumb+1.0Thumb
1.0Water + 1.0Palmitoyl-ACP (n-C16:0ACP) → 1.0acyl carrier protein + 1.0Hydrogen ion + 1.0Palmitic acid
ReactionCard
1.0Holo-[acyl-carrier-protein]+1.0Thumb → 1.0Thumb+1.0apo-[acyl-carrier-protein]
1.0Holo-[acyl-carrier-protein] + 1.0Water → 1.0Pantetheine 4'-phosphate + 1.0apo-[acyl-carrier-protein]
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00482Caprylic acidMetaboCard
ECMDB21204Decanoate (N-C10:0)MetaboCard
ECMDB21209Dodecanoate (N-C12:0)MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00220Palmitic acidMetaboCard
ECMDB01416Pantetheine 4'-phosphateMetaboCard
ECMDB21361tetradecanoate (n-C14:0)MetaboCard
ECMDB21362Tetradecenoate (N-C14:1)MetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on ester bonds
phosphoric diester hydrolase activity
phosphoric ester hydrolase activity
[acyl-carrier-protein] phosphodiesterase activity
Process
carboxylic acid metabolic process
cellular metabolic process
fatty acid biosynthetic process
fatty acid metabolic process
metabolic process
monocarboxylic acid metabolic process
organic acid metabolic process
oxoacid metabolic process
Gene Properties
Blattner:b0404
Gene OrientationCounterclockwise
Centisome Percentage:9.13
Left Sequence End423561
Right Sequence End424142
Gene Sequence:
>582 bp
ATGAATTTTTTAGCTCACCTGCATTTAGCCCATCTCGCGGAAAGCTCGCTTTCCGGCAAT
TTACTGGCTGATTTCGTACGCGGAAATCCTGAAGAAAGTTTTCCGCCCGACGTCGTGGCT
GGCATTCATATGCATCGACGTATCGACGTATTGACTGACAATCTGCCGGAAGTCCGCGAA
GCACGGGAGTGGTTTCGTAGTGAAACGCGCCGCGTTGCGCCTATTACGCTGGATGTCATG
TGGGATCACTTTCTTTCCCGCCACTGGTCGCAGCTGTCGCCGGACTTTCCGCTACAGGAA
TTTGTCTGTTATGCCCGCGAGCAAGTGATGACGATTTTGCCGGACTCACCGCCACGTTTT
ATCAATCTGAACAATTACTTGTGGTCAGAGCAGTGGCTGGTGCGCTATCGCGATATGGAT
TTCATCCAGAACGTGTTAAACGGCATGGCAAGCCGCCGCCCACGTCTGGATGCCCTGCGT
GACTCCTGGTACGATTTAGACGCTCATTATGACGCCCTCGAAACCCGCTTCTGGCAGTTT
TATCCGCGGATGATGGCGCAGGCGTCACGCAAGGCGTTATAA
Protein Properties
Pfam Domain Function:
Protein Residues:193
Protein Molecular Weight:22961
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Acyl carrier protein phosphodiesterase
MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHMHRRIDVLTDNLPEVRE
AREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFVCYAREQVMTILPDSPPRF
INLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALRDSWYDLDAHYDALETRFWQF
YPRMMAQASRKAL
References
External Links:
ResourceLink
Uniprot ID:P21515
Uniprot Name:ACPH_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674544
Ecogene ID:EG11095
Ecocyc:EG11095
ColiBase:b0404
Kegg Gene:b0404
EchoBASE ID:EB1087
CCDB:ACPH_ECOLI
BacMap:16128389
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Reuter, K., Slany, R., Ullrich, F., Kersten, H. (1991). "Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes." J Bacteriol 173:2256-2264. Pubmed: 1706703
  • Thomas, J., Cronan, J. E. (2005). "The enigmatic acyl carrier protein phosphodiesterase of Escherichia coli: genetic and enzymological characterization." J Biol Chem 280:34675-34683. Pubmed: 16107329