ECMDB: Spermidine N(1)-acetyltransferase (P0A951)

Identification

Name:

Spermidine N(1)-acetyltransferase

Synonyms:

Diamine acetyltransferase
SAT

Gene Name:

speG

Enzyme Class:

2.3.1.57

Biological Properties

General Function:

Involved in N-acetyltransferase activity

Specific Function:

Regulates polyamine concentration by their degradation. Acts on spermidine, forming N(1)- and N(8)-acetylspermidine

Cellular Location:

Cytoplasm (Potential)

SMPDB Pathways:

S-adenosyl-L-methionine biosynthesis PW000837

KEGG Pathways:

Arginine and proline metabolism ec00330
Metabolic pathways eco01100

KEGG Reactions:

1.0

↔

1.0

1.0Acetyl-CoA + 1.0Putrescine ↔ 1.0Coenzyme A + 1.0N-Acetylputrescine
ReactionCard

1.0

↔

1.0

1.0Acetyl-CoA + 1.0Alkane-alpha,omega-diamine ↔ 1.0Coenzyme A + 1.0N-Acetyldiamine
ReactionCard

EcoCyc Reactions:

1.0

→

1.0

1.0Acetyl-CoA + 1.0Spermidine → 1.0N1-Acetylspermidine + 1.0Coenzyme A + 1.0Hydrogen ion
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Acetyl-CoA + 1.0Spermidine → 1.0Coenzyme A + 1.0Hydrogen ion + 1.0N8-Acetylspermidine
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01206	Acetyl-CoA	MetaboCard
ECMDB01423	Coenzyme A	MetaboCard
ECMDB20274	Ethylenediamine	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB02064	N-Acetylputrescine	MetaboCard
ECMDB01276	N1-Acetylspermidine	MetaboCard
ECMDB21254	N8-Acetylspermidine	MetaboCard
ECMDB01414	Putrescine	MetaboCard
ECMDB01257	Spermidine	MetaboCard

GO Classification:

Function
acetyltransferase activity
acyltransferase activity
catalytic activity
N-acetyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
metabolic process

Gene Properties

Blattner:

b1584

Gene Orientation

Clockwise

Centisome Percentage:

35.65

Left Sequence End

1654208

Right Sequence End

1654768

Gene Sequence:

>561 bp
ATGAACGTGAGTAAATATGTCGCTATCTTTTCCTTTGTTTTTATTCAGTTAATCAGCGTT
GGTAAAGTTTTTGCTAACGCAGATGAGTGGATGACAACGTTTAGAGAAAATATTGCACAA
ACCTGGCAACAGCCTGAACATTATGATTTATATATTCCTGCCATCACCTGGCATGCACGT
TTCGCTTACGACAAAGAAAAAACCGATCGCTATAACGAGCGACCGTGGGGTGGCGGTTTT
GGCCTGTCGCGTTGGGATGAAAAAGGAAACTGGCATGGCCTGTATGCCATGGCATTTAAG
GACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCG
CTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGAT
AACTGGAATTACATCCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCA
GTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCC
TGGATGCGCTTTCAGTTTTGA

Protein Properties

Pfam Domain Function:

Acetyltransf_1 (PF00583 )

Protein Residues:

186

Protein Molecular Weight:

21887

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Spermidine N(1)-acetyltransferase
MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVELSDLYDKHIHDQSERRF
VVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVLNLYKL
YLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAIRMCIFQHQYLAEHKTPGQTL
LKPTAQ

References

External Links:

Resource	Link
Uniprot ID:	P0A951
Uniprot Name:	ATDA_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674727
Ecogene ID:	EG12447
Ecocyc:	EG12447
ColiBase:	b1584
Kegg Gene:	b1584
EchoBASE ID:	EB2341
CCDB:	ATDA_ECOLI
BacMap:	16129542

General Reference:

Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Fukuchi, J., Kashiwagi, K., Takio, K., Igarashi, K. (1994). "Properties and structure of spermidine acetyltransferase in Escherichia coli." J Biol Chem 269:22581-22585. Pubmed: 8077207
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553