ECMDB

Identification

Name:

Uridine kinase

Synonyms:

Cytidine monophosphokinase
Uridine monophosphokinase

Gene Name:

udk

Enzyme Class:

2.7.1.48

Biological Properties

General Function:

Involved in ATP binding

Specific Function:

ATP + uridine = ADP + UMP

Cellular Location:

Cytoplasm

SMPDB Pathways:

Pyrimidine metabolism PW000942

KEGG Pathways:

Drug metabolism - other enzymes ec00983
Metabolic pathways eco01100
Pyrimidine metabolism ec00240

KEGG Reactions:

1.0

→

1.0

1.0Cytidine + 1.0Guanosine triphosphate → 1.0Cytidine monophosphate + 1.0Guanosine diphosphate + 1.0Hydrogen ion
ReactionCard

1.0

→

1.0

1.0Guanosine triphosphate + 1.0Uridine → 1.0Guanosine diphosphate + 1.0Hydrogen ion + 1.0Uridine 5'-monophosphate
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1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Cytidine ↔ 1.0ADP + 1.0Cytidine monophosphate
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1.0

↔

1.0

1.0Uridine triphosphate + 1.0Cytidine ↔ 1.0Uridine 5'-diphosphate + 1.0Cytidine monophosphate
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1.0

↔

1.0

1.0Guanosine triphosphate + 1.0Cytidine ↔ 1.0Guanosine diphosphate + 1.0Cytidine monophosphate
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1.0

↔

1.0

1.0Inosine triphosphate + 1.0Cytidine ↔ 1.0IDP + 1.0Cytidine monophosphate
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1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Uridine ↔ 1.0ADP + 1.0Uridine 5'-monophosphate
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1.0

↔

1.0

1.0Uridine triphosphate + 1.0Uridine ↔ 1.0Uridine 5'-diphosphate + 1.0Uridine 5'-monophosphate
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1.0

↔

1.0

1.0Guanosine triphosphate + 1.0Uridine ↔ 1.0Guanosine diphosphate + 1.0Uridine 5'-monophosphate
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1.0

↔

1.0

1.0Inosine triphosphate + 1.0Uridine ↔ 1.0IDP + 1.0Uridine 5'-monophosphate
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1.0

↔

1.0

1.0dATP + 1.0Cytidine ↔ 1.0dADP + 1.0Cytidine monophosphate
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1.0

↔

1.0

1.0dATP + 1.0Uridine ↔ 1.0dADP + 1.0Uridine 5'-monophosphate
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1.0

↔

1.0dGDP

1.0

1.0dGTP + 1.0Uridine ↔ 1.0dGDP + 1.0Uridine 5'-monophosphate
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1.0

↔

1.0dGDP

1.0

1.0dGTP + 1.0Cytidine ↔ 1.0dGDP + 1.0Cytidine monophosphate
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1.0

↔

1.0

1.0Thymidine 5'-triphosphate + 1.0Cytidine ↔ 1.0dTDP + 1.0Cytidine monophosphate
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1.0

↔

1.0

1.0Thymidine 5'-triphosphate + 1.0Uridine ↔ 1.0dTDP + 1.0Uridine 5'-monophosphate
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1.0

↔

1.0

1.0dCTP + 1.0Uridine ↔ 1.0dCDP + 1.0Uridine 5'-monophosphate
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1.0

↔

1.0dUDP

1.0

1.0Deoxyuridine triphosphate + 1.0Uridine ↔ 1.0dUDP + 1.0Uridine 5'-monophosphate
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1.0

↔

1.0

1.0dCTP + 1.0Cytidine ↔ 1.0dCDP + 1.0Cytidine monophosphate
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1.0

↔

1.0dUDP

1.0

1.0Deoxyuridine triphosphate + 1.0Cytidine ↔ 1.0dUDP + 1.0Cytidine monophosphate
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1.05-Fluorouridine

1.0

↔

1.05-Fluorouridine monophosphate

1.0

1.05-Fluorouridine + 1.0Adenosine triphosphate ↔ 1.05-Fluorouridine monophosphate + 1.0ADP
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EcoCyc Reactions:

1.0

→

1.0

1.0Cytidine + 1.0Guanosine triphosphate → 1.0Cytidine monophosphate + 1.0Guanosine diphosphate + 1.0Hydrogen ion
ReactionCard

1.0

→

1.0

1.0Guanosine triphosphate + 1.0Uridine → 1.0Guanosine diphosphate + 1.0Hydrogen ion + 1.0Uridine 5'-monophosphate
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Uridine → 1.0ADP + 1.0Uridine 5'-monophosphate
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1.0

→

1.0

1.0Adenosine triphosphate + 1.0Cytidine → 1.0ADP + 1.0Cytidine monophosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB00089	Cytidine	MetaboCard
ECMDB00095	Cytidine monophosphate	MetaboCard
ECMDB21326	dADP	MetaboCard
ECMDB01532	dATP	MetaboCard
ECMDB01245	dCDP	MetaboCard
ECMDB00998	dCTP	MetaboCard
ECMDB01191	Deoxyuridine triphosphate	MetaboCard
ECMDB00960	dGDP	MetaboCard
ECMDB01440	dGTP	MetaboCard
ECMDB01274	dTDP	MetaboCard
ECMDB01000	dUDP	MetaboCard
ECMDB01201	Guanosine diphosphate	MetaboCard
ECMDB01273	Guanosine triphosphate	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB03335	IDP	MetaboCard
ECMDB00189	Inosine triphosphate	MetaboCard
ECMDB01342	Thymidine 5'-triphosphate	MetaboCard
ECMDB00296	Uridine	MetaboCard
ECMDB00295	Uridine 5'-diphosphate	MetaboCard
ECMDB00288	Uridine 5'-monophosphate	MetaboCard
ECMDB00285	Uridine triphosphate	MetaboCard

GO Classification:

Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
kinase activity
nucleoside binding
phosphotransferase activity, alcohol group as acceptor
purine nucleoside binding
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
metabolic process

Gene Properties

Blattner:

b2066

Gene Orientation

Counterclockwise

Centisome Percentage:

46.13

Left Sequence End

2140331

Right Sequence End

2140972

Gene Sequence:

>642 bp
ATGCGCAGTAAGTATATCGTCATTGAGGGGCTGGAAGGCGCAGGCAAAACTACCGCGCGT
AATGTGGTGGTTGAGACGCTCGAGCAACTGGGTATCCGCGACATGGTTTTCACTCGGGAA
CCTGGCGGTACGCAACTTGCCGAAAAGTTAAGAAGCCTGGTGCTGGATATCAAATCGGTA
GGCGATGAAGTCATTACCGATAAAGCCGAAGTTCTGATGTTTTATGCCGCGCGCGTTCAA
CTGGTAGAAACGGTCATCAAACCAGCTCTGGCTAACGGCACCTGGGTGATTGGCGATCGC
CACGATCTCTCCACTCAGGCGTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTG
GCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGAT
GTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAG
CAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAA
GATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATC
CGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGA

Protein Properties

Pfam Domain Function:

PRK (PF00485 )

Protein Residues:

213

Protein Molecular Weight:

24353

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Uridine kinase
MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVIPEDCYYKDQSHLSMEE
RVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTRMKETVTVEPKKVIILEGI
LLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRSMDSVMAQYQKTVRPMFLQFI
EPSKQYADIIVPRGGKNRIAIDILKAKISQFFE

References

External Links:

Resource	Link
Uniprot ID:	P0A8F4
Uniprot Name:	URK_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	4062665
Ecogene ID:	EG11701
Ecocyc:	EG11701
ColiBase:	b2066
Kegg Gene:	b2066
EchoBASE ID:	EB1652
CCDB:	URK_ECOLI
BacMap:	90111379

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
Neuhard, J., Tarpo, L. (1993). "Location of the udk gene on the physical map of Escherichia coli." J Bacteriol 175:5742-5743. Pubmed: 8366067

Uridine kinase (P0A8F4)