ECMDB

Identification

Name:

Bifunctional aspartokinase/homoserine dehydrogenase 2

Synonyms:

Aspartokinase II/homoserine dehydrogenase II
AKII-HDII
Aspartokinase
Homoserine dehydrogenase

Gene Name:

metL

Enzyme Class:

Biological Properties

General Function:

Involved in cellular amino acid biosynthetic process

Specific Function:

L-homoserine + NAD(P)(+) = L-aspartate 4- semialdehyde + NAD(P)H

Cellular Location:

Not Available

SMPDB Pathways:

Lysine biosynthesis PW000771
Secondary Metabolites: threonine biosynthesis from aspartate PW000976
methionine biosynthesis PW000814
threonine biosynthesis PW000817

KEGG Pathways:

Cysteine and methionine metabolism ec00270
Glycine, serine and threonine metabolism ec00260
Lysine biosynthesis ec00300
Metabolic pathways eco01100
Microbial metabolism in diverse environments ec01120
Monobactam biosynthesis eco00261

KEGG Reactions:

1.0

↔

1.0

1.0L-Aspartic acid + 1.0Adenosine triphosphate ↔ 1.0L-Aspartyl-4-phosphate + 1.0ADP
ReactionCard

1.0

↔

1.0

1.0L-Homoserine + 1.0NADP ↔ 1.0L-Aspartate-semialdehyde + 1.0Hydrogen ion + 1.0NADPH
ReactionCard

1.0

↔

1.0

1.0L-Homoserine + 1.0NAD ↔ 1.0L-Aspartate-semialdehyde + 1.0NADH + 1.0Hydrogen ion
ReactionCard

SMPDB Reactions:

1.0

1.0NADPH

1.0

→

1.0

1.0L-Homoserine

1.0

1.0L-Aspartate-semialdehyde + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0NADP + 1.0L-Homoserine + 1.0L-Homoserine
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0L-Aspartic acid + 1.0Adenosine triphosphate ↔ 1.0L-Aspartyl-4-phosphate + 1.0ADP
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB12249	L-Aspartate-semialdehyde	MetaboCard
ECMDB00191	L-Aspartic acid	MetaboCard
ECMDB12250	L-Aspartyl-4-phosphate	MetaboCard
ECMDB02251	L-Homoserine	MetaboCard
ECMDB00902	NAD	MetaboCard
ECMDB01487	NADH	MetaboCard
ECMDB00217	NADP	MetaboCard
ECMDB04111	NADPH	MetaboCard

GO Classification:

Function
aspartate kinase activity
binding
catalytic activity
homoserine dehydrogenase activity
NADP or NADPH binding
nucleotide binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
phosphotransferase activity, carboxyl group as acceptor
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
aspartate family amino acid biosynthetic process
cellular amino acid and derivative metabolic process
cellular amino acid biosynthetic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process
oxidation reduction

Gene Properties

Blattner:

b3940

Gene Orientation

Clockwise

Centisome Percentage:

88.97

Left Sequence End

4127858

Right Sequence End

4130290

Gene Sequence:

>2433 bp
ATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCACCGTATACGGT
GCTGAAGGGTTCGTAGTGAAAGATATTCATTTCGAAGGCCTTCAGCGTGTCGCCGTTGGT
GCGGCCCTCCTCAGTATGCCGGTGCGCACAGGCGACACGGTTAATGATGAAGATATCAGT
AATACCATTCGCGCTCTGTTTGCTACCGGCAACTTTGAGGATGTTCGCGTCCTTCGTGAT
GGTGATACCCTTCTGGTTCAGGTAAAAGAACGTCCGACCATTGCCAGCATTACTTTCTCC
GGTAACAAATCGGTGAAAGATGACATGCTGAAGCAAAACCTCGAGGCTTCTGGTGTGCGT
GTGGGCGAATCCCTCGATCGCACCACCATTGCCGATATCGAGAAAGGTCTGGAAGACTTC
TACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGC
AACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATT
AACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGATCTCTCATTTCCAACTGCGT
GACGAAGTGCCGTGGTGGAACGTGGTAGGCGATCGTAAATACCAGAAACAGAAACTGGCG
GGCGACCTTGAAACCCTGCGCAGCTACTATCTGGATCGCGGTTATGCCCGTTTCAACATC
GACTCTACCCAGGTCAGTCTGACGCCAGATAAAAAAGGTATTTACGTCACGGTGAACATC
ACCGAAGGCGATCAGTACAAGCTTTCTGGCGTTGAAGTGAGCGGCAACCTTGCCGGGCAC
TCCGCTGAAATTGAGCAGCTGACTAAGATCGAGCCGGGTGAGCTGTATAACGGCACCAAA
GTGACCAAGATGGAAGATGACATCAAAAAGCTTCTCGGTCGCTATGGTTATGCCTATCCG
CGCGTACAGTCGATGCCCGAAATTAACGATGCCGACAAAACCGTTAAATTACGTGTGAAC
GTTGATGCGGGTAACCGTTTCTACGTGCGTAAGATCCGTTTTGAAGGTAACGATACCTCG
AAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGAT
CTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACC
GATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAG
CGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTC
CAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGG
ACCAAAAACGATTACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTA
GATGGCGTAAGCCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACGCCGAC
CTGTCCGACTATACCAACAAGAGTTATGGTACAGACGTGACGTTGGGCTTCCCGATTAAC
GAATATAACTCGCTGCGTGCAGGTCTGGGTTATGTACATAACTCCCTGTCCAACATGCAG
CCTCAGGTTGCGATGTGGCGTTATCTGTACTCTATGGGTGAACATCCGAGCACCTCTGAT
CAGGATAACAGCTTCAAAACGGACGACTTCACGTTCAACTATGGTTGGACCTATAACAAG
CTTGACCGTGGTTACTTCCCGACAGATGGTTCACGTGTCAACCTGACCGGTAAAGTGACC
ATTCCTGGATCGGATAACGAATACTACAAAGTGACGTTAGACACGGCGACTTATGTGCCG
ATCGATGACGATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGT
TTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTG
CGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGT
AATTATGATCCGGACTATGATTACGAATGTGCGACTCAGGACGGCGCGAAAGACCTGTGT
AAATCGGATGATGCTGTAGGCGGTAACGCCATGGCGGTTGCCAGCCTCGAGTTCATCACC
CCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGAT
ATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTAT
AGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTG
GGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAA
CAGTTCCAGTTTAACATCGGTAAAACCTGGTAA

Protein Properties

Pfam Domain Function:

AA_kinase (PF00696 )
Homoserine_dh (PF00742 )
NAD_binding_3 (PF03447 )

Protein Residues:

810

Protein Molecular Weight:

88887

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Bifunctional aspartokinase/homoserine dehydrogenase 2
MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINW
LKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDA
VYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAERAAQPQVDEGLSYPLLQQLL
VQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP
RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIER
VLASGTGARIVTSHDDVCLIEFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLL
QFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAGVTRNPLHCHRFWQQLKGQPV
EFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFARE
QSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPY
DDLVVLDVTASQQLADQYLDFASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYN
ATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLT
EPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNE
QMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRD
NPLVIRGPGAGRDVTAGAIQSDINRLAQLL

References

External Links:

Resource	Link
Uniprot ID:	P00562
Uniprot Name:	AK2H_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674369
Ecogene ID:	EG10590
Ecocyc:	EG10590
ColiBase:	b3940
Kegg Gene:	b3940
EchoBASE ID:	EB0585
CCDB:	AK2H_ECOLI
BacMap:	16131778

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Plunkett, G. 3rd, Burland, V., Daniels, D. L., Blattner, F. R. (1993). "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Nucleic Acids Res 21:3391-3398. Pubmed: 8346018
Zakin, M. M., Duchange, N., Ferrara, P., Cohen, G. N. (1983). "Nucleotide sequence of the metL gene of Escherichia coli. Its product, the bifunctional aspartokinase ii-homoserine dehydrogenase II, and the bifunctional product of the thrA gene, aspartokinase I-homoserine dehydrogenase I, derive from a common ancestor." J Biol Chem 258:3028-3031. Pubmed: 6298218

Bifunctional aspartokinase/homoserine dehydrogenase 2 (P00562)