2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase (P77044)

Identification
Name:2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase
Synonyms:
  • 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase
  • 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase
  • 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase
Gene Name:mhpC
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Microbial metabolism in diverse environments ec01120
  • Phenylalanine metabolism ec00360
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Water + 1.02-Hydroxy-6-ketononadienedicarboxylate → 1.0Hydrogen ion + 1.02-Hydroxy-2,4-pentadienoate + 1.0Succinic acid
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Water + 1.02-Hydroxy-6-ketononatrienedioate → 1.0Fumaric acid + 1.0Hydrogen ion + 1.02-Hydroxy-2,4-pentadienoate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-Hydroxy-2,4-pentadienoate + 1.0Succinic acid ↔ 1.02-Hydroxy-6-ketononadienedicarboxylate + 1.0Water
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-Hydroxy-6-ketononatrienedioate + 1.0Water ↔ 1.02-Hydroxy-2,4-pentadienoate + 1.0Fumaric acid
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-Hydroxy-6-ketononadienedicarboxylate + 1.0Water + 1.02-Hydroxy-6-ketononatrienedioate ↔ 1.0Succinic acid + 1.0Fumaric acid
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.02-Hydroxy-2,4-pentadienoate
1.02-Hydroxy-6-ketononatrienedioate + 1.0Water → 1.0Hydrogen ion + 1.0Fumaric acid + 1.02-Hydroxy-2,4-pentadienoate + 1.02-Hydroxy-2,4-pentadienoate
ReactionCard
1.0(2E,4Z)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate+1.0Thumb → 1.0(2Z)-2-hydroxypenta-2,4-dienoate+1.0Thumb+1.0Thumb
1.0(2E,4Z)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + 1.0Water → 1.0(2Z)-2-hydroxypenta-2,4-dienoate + 1.0Succinic acid + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Water + 1.02-Hydroxy-6-ketononadienedicarboxylate → 1.0Hydrogen ion + 1.02-Hydroxy-2,4-pentadienoate + 1.0Succinic acid
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Water + 1.02-Hydroxy-6-ketononatrienedioate → 1.0Fumaric acid + 1.0Hydrogen ion + 1.02-Hydroxy-2,4-pentadienoate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-Hydroxy-2,4-pentadienoate + 1.0Succinic acid ↔ 1.02-Hydroxy-6-ketononadienedicarboxylate + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB200412-Hydroxy-2,4-pentadienoateMetaboCard
ECMDB040812-Hydroxy-6-ketononadienedicarboxylateMetaboCard
ECMDB200422-Hydroxy-6-ketononatrienedioateMetaboCard
ECMDB00176Fumaric acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00254Succinic acidMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
Gene Properties
Blattner:b0349
Gene OrientationClockwise
Centisome Percentage:7.98
Left Sequence End370463
Right Sequence End371329
Gene Sequence:
>882 bp
ATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCTG
AATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGAC
GAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGTGCTACTGGCTGGGCGAACTTCAGC
CGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGT
TGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATC
CTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATG
GGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTG
CTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATT
AAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATGGAT
ATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAAT
ATGCTGTCGCGCCGCGATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAA
CAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGCGCAAACCCTGATTGTCTGG
GGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCC
GGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGAC
GCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:293
Protein Molecular Weight:32585
Protein Theoretical pI:7
PDB File:1U2E
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase
MQEKMMSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGSGPGATGWANFS
RNIDPLVEAGYRVILLDCPGWGKSDSVVNSGSRSDLNARILKSVVDQLDIAKIHLLGNSM
GGHSSVAFTLKWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQPTIENLKLMMD
IFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPRLAEIKAQTLIVW
GRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLVLNFLARP
References
External Links:
ResourceLink
Uniprot ID:P77044
Uniprot Name:MHPC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674491
PDB ID:1U2E
Ecogene ID:EG20275
Ecocyc:EG20275
ColiBase:b0349
Kegg Gene:b0349
EchoBASE ID:EB4168
CCDB:MHPC_ECOLI
BacMap:90111115
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Dunn, G., Montgomery, M. G., Mohammed, F., Coker, A., Cooper, J. B., Robertson, T., Garcia, J. L., Bugg, T. D., Wood, S. P. (2005). "The structure of the C-C bond hydrolase MhpC provides insights into its catalytic mechanism." J Mol Biol 346:253-265. Pubmed: 15663942
  • Ferrandez, A., Garcia, J. L., Diaz, E. (1997). "Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12." J Bacteriol 179:2573-2581. Pubmed: 9098055
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Li, C., Li, J. J., Montgomery, M. G., Wood, S. P., Bugg, T. D. (2006). "Catalytic role for arginine 188 in the C-C hydrolase catalytic mechanism for Escherichia coli MhpC and Burkholderia xenovorans LB400 BphD." Biochemistry 45:12470-12479. Pubmed: 17029402
  • Li, C., Montgomery, M. G., Mohammed, F., Li, J. J., Wood, S. P., Bugg, T. D. (2005). "Catalytic mechanism of C-C hydrolase MhpC from Escherichia coli: kinetic analysis of His263 and Ser110 site-directed mutants." J Mol Biol 346:241-251. Pubmed: 15663941