3-ketoacyl-CoA thiolase_ (P76503)

Identification
Name:3-ketoacyl-CoA thiolase_
Synonyms:
  • ACSs
  • Acetyl-CoA acyltransferase
  • Acyl-CoA ligase
  • Beta-ketothiolase
  • Fatty acid oxidation complex subunit beta
Gene Name:fadI
Enzyme Class:
Biological Properties
General Function:Involved in transferase activity
Specific Function:Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed. Strongly involved in the anaerobic degradation of long and medium-chain fatty acids in the presence of nitrate and weakly involved in the aerobic degradation of long- chain fatty acids
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
  • Benzoate degradation via hydroxylation ec00362
  • Biosynthesis of unsaturated fatty acids ec01040
  • Ethylbenzene degradation ec00642
  • Fatty acid elongation in mitochondria ec00062
  • Fatty acid metabolism ec00071
  • Geraniol degradation ec00281
  • Metabolic pathways eco01100
  • Microbial metabolism in diverse environments ec01120
  • Valine, leucine and isoleucine degradation ec00280
  • alpha-Linolenic acid metabolism ec00592
KEGG Reactions:
2.0Thumb ↔ 1.0Thumb+1.0Thumb
2.0Acetyl-CoA ↔ 1.0Acetoacetyl-CoA + 1.0Coenzyme A
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Octanoyl-CoA ↔ 1.03-Oxodecanoyl-CoA + 1.0Coenzyme A
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Tetradecanoyl-CoA ↔ 1.03-Oxohexadecanoyl-CoA + 1.0Coenzyme A
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Hexanoyl-CoA ↔ 1.03-Oxooctanoyl-CoA + 1.0Coenzyme A
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Lauroyl-CoA ↔ 1.03-Oxotetradecanoyl-CoA + 1.0Coenzyme A
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Decanoyl-CoA (N-C10:0CoA) ↔ 1.03-Oxododecanoyl-CoA + 1.0Coenzyme A
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Coenzyme A + 1.03-Oxoadipyl-CoA ↔ 1.0Acetyl-CoA + 1.0Succinyl-CoA
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Succinyl-CoA + 1.0Acetyl-CoA ↔ 1.0Coenzyme A + 1.03-Oxoadipyl-CoA
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Propionyl-CoA + 1.0Acetyl-CoA ↔ 1.0Coenzyme A + 1.02-Methylacetoacetyl-CoA
ReactionCard
1.0Thumb+1.0Butanoyl-CoA ↔ 1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Butanoyl-CoA ↔ 1.0Coenzyme A + 1.03-Oxohexanoyl-CoA
ReactionCard
1.0Thumb+1.0Chenodeoxycholoyl-CoA ↔ 1.0Thumb+1.0Thumb
1.0Propionyl-CoA + 1.0Chenodeoxycholoyl-CoA ↔ 1.0Coenzyme A + 1.03alpha,7alpha-Dihydroxy-5beta-cholestanoyl-CoA
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.07-Methyl-3-oxo-6-octenoyl-CoA + 1.0Coenzyme A ↔ 1.05-Methylhex-4-enoyl-CoA + 1.0Acetyl-CoA
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.05-Methyl-3-oxo-4-hexenoyl-CoA + 1.0Coenzyme A ↔ 1.03-Methylcrotonyl-CoA + 1.0Acetyl-CoA
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1.0Acyl-CoA+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Acyl-CoA + 1.0Acetyl-CoA ↔ 1.0Coenzyme A + 1.03-Oxoacyl-CoA
ReactionCard
SMPDB Reactions:
1.03-oxo-opc8-coa → 1.0OPC6-CoA
1.03-oxo-opc8-coa → 1.0OPC6-CoA
ReactionCard
1.03-Oxo-OPC6-CoA → 1.0OPC4-CoA
1.03-Oxo-OPC6-CoA → 1.0OPC4-CoA
ReactionCard
1.03-oxo-opc4-coa → 1.0(+)-7-Isojasmonic acid CoA
1.03-oxo-opc4-coa → 1.0(+)-7-Isojasmonic acid CoA
ReactionCard
1.0a 3-oxoacyl-CoA +1.0Thumb → 1.0Thumb+1.0a 2,3,4-saturated fatty acyl CoA 
1.0a 3-oxoacyl-CoA  + 1.0Coenzyme A → 1.0Acetyl-CoA + 1.0a 2,3,4-saturated fatty acyl CoA 
ReactionCard
1.0Acetoacetyl-CoA+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Succinyl-CoA+1.0Thumb
1.0Acetoacetyl-CoA + 1.0Coenzyme A + 1.0Acetoacetyl-CoA → 1.0Acetyl-CoA + 1.0Succinyl-CoA + 1.0Succinyl-CoA
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.03-Oxodecanoyl-CoA + 1.0Coenzyme A → 1.0Acetyl-CoA + 1.0Lauroyl-CoA
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Octanoyl-CoA+1.0Thumb
1.03-Oxohexanoyl-CoA + 1.0Coenzyme A → 1.0Acetyl-CoA + 1.0Octanoyl-CoA + 1.0Octanoyl-CoA
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.03-Oxododecanoyl-CoA + 1.0Coenzyme A → 1.0Acetyl-CoA + 1.0Tetradecanoyl-CoA
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.03-Oxotetradecanoyl-CoA + 1.0Coenzyme A → 1.0Acetyl-CoA + 1.0Palmityl-CoA
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Decanoyl-CoA (n-C10:0CoA)+1.0Thumb
1.03-Oxooctanoyl-CoA + 1.0Coenzyme A → 1.0Acetyl-CoA + 1.0Decanoyl-CoA (n-C10:0CoA) + 1.0Decanoyl-CoA (N-C10:0CoA)
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Stearoyl-CoA+1.0Thumb
1.03-Oxohexadecanoyl-CoA + 1.0Coenzyme A → 1.0Acetyl-CoA + 1.0Stearoyl-CoA + 1.0Stearoyl-CoA
ReactionCard
1.03-Oxooctadecanoyl-CoA+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Stearoyl-CoA+1.0Thumb
1.03-Oxooctadecanoyl-CoA + 1.0Coenzyme A + 1.03-Oxooctadecanoyl-CoA → 1.0Acetyl-CoA + 1.0Stearoyl-CoA + 1.0Stearoyl-CoA
ReactionCard
EcoCyc Reactions:
2.0Thumb ↔ 1.0Thumb+1.0Thumb
2.0Acetyl-CoA ↔ 1.0Acetoacetyl-CoA + 1.0Coenzyme A
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Coenzyme A + 1.03-Oxoadipyl-CoA ↔ 1.0Acetyl-CoA + 1.0Succinyl-CoA
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Butyryl-CoA ↔ 1.03-Oxohexanoyl-CoA + 1.0Coenzyme A
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.03-Oxooctadecanoyl-CoA + 1.0Coenzyme A ↔ 1.0Acetyl-CoA + 1.0Palmityl-CoA
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB011572-Methylacetoacetyl-CoAMetaboCard
ECMDB014933-Methylcrotonyl-CoAMetaboCard
ECMDB200743-Oxo-OPC4-CoAMetaboCard
ECMDB200753-Oxo-OPC6-CoAMetaboCard
ECMDB200773-Oxoadipyl-CoAMetaboCard
ECMDB039393-Oxodecanoyl-CoAMetaboCard
ECMDB039373-Oxododecanoyl-CoAMetaboCard
ECMDB064023-Oxohexadecanoyl-CoAMetaboCard
ECMDB039433-Oxohexanoyl-CoAMetaboCard
ECMDB211703-Oxooctadecanoyl-CoAMetaboCard
ECMDB039413-Oxooctanoyl-CoAMetaboCard
ECMDB039353-Oxotetradecanoyl-CoAMetaboCard
ECMDB021593alpha,7alpha-Dihydroxy-5beta-cholestanoyl-CoAMetaboCard
ECMDB201045-Methyl-3-oxo-4-hexenoyl-CoAMetaboCard
ECMDB201055-Methylhex-4-enoyl-CoAMetaboCard
ECMDB201087-Methyl-3-oxo-6-octenoyl-CoAMetaboCard
ECMDB01484Acetoacetyl-CoAMetaboCard
ECMDB01206Acetyl-CoAMetaboCard
ECMDB20120Benzoyl acetyl-CoAMetaboCard
ECMDB01088Butyryl-CoAMetaboCard
ECMDB01423Coenzyme AMetaboCard
ECMDB06404Decanoyl-CoA (N-C10:0CoA)MetaboCard
ECMDB02845Hexanoyl-CoAMetaboCard
ECMDB03571Lauroyl-CoAMetaboCard
ECMDB01070Octanoyl-CoAMetaboCard
ECMDB01338Palmityl-CoAMetaboCard
ECMDB01275Propionyl-CoAMetaboCard
ECMDB21284Stearoyl-CoAMetaboCard
ECMDB01022Succinyl-CoAMetaboCard
ECMDB01521Tetradecanoyl-CoAMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
acyltransferase activity
catalytic activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
carboxylic acid metabolic process
cellular metabolic process
fatty acid metabolic process
lipid catabolic process
lipid metabolic process
metabolic process
monocarboxylic acid metabolic process
organic acid metabolic process
oxoacid metabolic process
primary metabolic process
Gene Properties
Blattner:b2342
Gene OrientationCounterclockwise
Centisome Percentage:52.96
Left Sequence End2457181
Right Sequence End2458491
Gene Sequence:
>1311 bp
ATGGGTCAGGTTTTACCGCTGGTTACCCGCCAGGGCGATCGTATCGCCATTGTTAGCGGT
TTACGTACGCCTTTTGCCCGTCAGGCGACGGCTTTTCATGGCATTCCCGCGGTTGATTTA
GGGAAGATGGTGGTAGGCGAACTGCTGGCACGCAGCGAGATCCCCGCCGAAGTGATTGAA
CAACTGGTCTTTGGTCAGGTCGTACAAATGCCTGAAGCCCCCAACATTGCGCGTGAAATT
GTTCTCGGTACGGGAATGAATGTACATACCGATGCTTACAGCGTCAGCCGCGCTTGCGCT
ACCAGTTTCCAGGCAGTTGCAAACGTCGCAGAAAGCCTGATGGCGGGAACTATTCGAGCG
GGGATTGCCGGTGGGGCAGATTCCTCTTCGGTATTGCCAATTGGCGTCAGTAAAAAACTG
GCGCGCGTGCTGGTTGATGTCAACAAAGCTCGTACCATGAGCCAGCGACTGAAACTCTTC
TCTCGCCTGCGTTTGCGCGACTTAATGCCCGTACCACCTGCGGTAGCAGAATATTCTACC
GGCTTGCGGATGGGCGACACCGCAGAGCAAATGGCGAAAACCTACGGCATCACCCGAGAA
CAGCAAGATGCATTAGCGCACCGTTCGCATCAGCGTGCCGCTCAGGCATGGTCAGACGGA
AAACTCAAAGAAGAGGTGATGACTGCCTTTATCCCTCCTTATAAACAACCGCTTGTCGAA
GACAACAATATTCGCGGTAATTCCTCGCTTGCCGATTACGCAAAGCTGCGCCCGGCGTTT
GATCGCAAACACGGAACGGTAACGGCGGCAAACAGTACGCCGCTGACCGATGGCGCGGCA
GCGGTGATCCTGATGACTGAATCCCGGGCGAAAGAATTAGGGCTGGTGCCGCTGGGGTAT
CTGCGCAGCTACGCATTTACTGCGATTGATGTCTGGCAGGACATGTTGCTCGGTCCAGCC
TGGTCAACACCGCTGGCGCTGGAGCGTGCCGGTTTGACGATGAGCGATCTGACATTGATC
GATATGCACGAAGCCTTTGCAGCTCAGACGCTGGCGAATATTCAGTTGCTGGGTAGTGAA
CGTTTTGCTCGTGAAGCACTGGGGCGTGCACATGCCACTGGCGAAGTGGACGATAGCAAA
TTTAACGTGCTTGGCGGTTCGATTGCTTACGGGCATCCCTTCGCGGCGACCGGCGCGCGG
ATGATTACCCAGACATTGCATGAACTTCGCCGTCGCGGCGGTGGATTTGGTTTAGTTACC
GCCTGTGCTGCCGGTGGGCTTGGCGCGGCAATGGTTCTGGAGGCGGAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:436
Protein Molecular Weight:46530
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>3-ketoacyl-CoA thiolase
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIE
QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
GIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSE
RFAREALGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT
ACAAGGLGAAMVLEAE
References
External Links:
ResourceLink
Uniprot ID:P76503
Uniprot Name:FADI_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675365
Ecogene ID:EG14128
Ecocyc:EG14128
ColiBase:b2342
Kegg Gene:b2342
EchoBASE ID:EB3880
CCDB:FADI_ECOLI
BacMap:16130275
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Campbell, J. W., Morgan-Kiss, R. M., Cronan, J. E. Jr (2003). "A new Escherichia coli metabolic competency: growth on fatty acids by a novel anaerobic beta-oxidation pathway." Mol Microbiol 47:793-805. Pubmed: 12535077
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Snell, K. D., Feng, F., Zhong, L., Martin, D., Madison, L. L. (2002). "YfcX enables medium-chain-length poly(3-hydroxyalkanoate) formation from fatty acids in recombinant Escherichia coli fadB strains." J Bacteriol 184:5696-5705. Pubmed: 12270828
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837