Glyoxylate/hydroxypyruvate reductase A (P75913)

Identification
Name:Glyoxylate/hydroxypyruvate reductase A
Synonyms:
  • 2-ketoacid reductase
Gene Name:ghrA
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Specific Function:Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively. Inactive towards 2-oxo-D-gluconate, 2-oxoglutarate, oxaloacetate and pyruvate. Only D- and L-glycerate are involved in the oxidative activity with NADP. Activity with NAD is very low
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:Not Available
KEGG Pathways:
  • Glycine, serine and threonine metabolism ec00260
  • Glyoxylate and dicarboxylate metabolism ec00630
  • Metabolic pathways eco01100
  • Microbial metabolism in diverse environments ec01120
  • Pyruvate metabolism ec00620
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Glyoxylic acid + 1.0Hydrogen ion + 1.0NADPH + 1.0Glycolate ↔ 1.0Glycolic acid + 1.0NADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Hydroxypyruvic acid + 1.0NADH → 1.0Glyceric acid + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Hydroxypyruvic acid + 1.0NADPH → 1.0Glyceric acid + 1.0NADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Pyruvaldehyde + 1.0NADH → 1.0D-Lactaldehyde + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glycolic acid + 1.0NADP ↔ 1.0Glyoxylic acid + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glyceric acid + 1.0NAD ↔ 1.0Hydroxypyruvic acid + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glyceric acid + 1.0NADP ↔ 1.0Hydroxypyruvic acid + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0D-Lactaldehyde + 1.0NAD ↔ 1.0Pyruvaldehyde + 1.0NADH + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Glyoxylic acid + 1.0Hydrogen ion + 1.0NADPH + 1.0Glycolate ↔ 1.0Glycolic acid + 1.0NADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Hydroxypyruvic acid + 1.0NADPH → 1.0Glyceric acid + 1.0NADP
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glycolic acid + 1.0NADP ↔ 1.0Glyoxylic acid + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ← 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glycolic acid + 1.0NADP ← 1.0Hydrogen ion + 1.0NADPH + 1.0Glyoxylic acid
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Glyoxylic acid + 1.0Hydrogen ion + 1.0NADH → 1.0Glycolic acid + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Glycolic acid + 1.0NADP → 1.0Glyoxylic acid + 1.0NADPH
ReactionCard
1.0Thumb+1.0NAD(P)(+) → 1.0Thumb+1.0NAD(P)H
1.0Glyceric acid + 1.0NAD(P)(+) → 1.0Hydroxypyruvic acid + 1.0NAD(P)H
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB06458D-LactaldehydeMetaboCard
ECMDB04077Glyceric acidMetaboCard
ECMDB23741GlycolateMetaboCard
ECMDB03035Glycolic acidMetaboCard
ECMDB00119Glyoxylic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01352Hydroxypyruvic acidMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
ECMDB01167PyruvaldehydeMetaboCard
GO Classification:
Function
binding
catalytic activity
cofactor binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Process
metabolic process
Gene Properties
Blattner:b1033
Gene OrientationClockwise
Centisome Percentage:23.65
Left Sequence End1097109
Right Sequence End1098047
Gene Sequence:
>939 bp
ATGGATATCATCTTTTATCACCCAACGTTCGATACCCAATGGTGGATTGAGGCACTGCGC
AAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGAT
TATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGATCTTAAAGCGGTG
TTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATG
CTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAG
GAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAG
CAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGC
ATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTT
CCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGA
CGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAAT
ACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCG
TATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTG
GATAGCGGCAAAGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCT
GAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACC
CGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAG
AGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:312
Protein Molecular Weight:35343
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glyoxylate/hydroxypyruvate reductase A
MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAV
FALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQ
QNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRFPLRCWSRTRKSWPGVQSFAG
REELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVEYISRTIAQLEKGE
RVCGQVDRARGY
References
External Links:
ResourceLink
Uniprot ID:P75913
Uniprot Name:GHRA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062597
Ecogene ID:EG13869
Ecocyc:EG13869
ColiBase:b1033
Kegg Gene:b1033
EchoBASE ID:EB3628
CCDB:GHRA_ECOLI
BacMap:90111205
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Fountoulakis, M., Takacs, M. F., Berndt, P., Langen, H., Takacs, B. (1999). "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Electrophoresis 20:2181-2195. Pubmed: 10493123
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Nunez, M. F., Pellicer, M. T., Badia, J., Aguilar, J., Baldoma, L. (2001). "Biochemical characterization of the 2-ketoacid reductases encoded by ycdW and yiaE genes in Escherichia coli." Biochem J 354:707-715. Pubmed: 11237876
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232