PTS system glucose-specific EIICB component (P69786)

Identification
Name:PTS system glucose-specific EIICB component
Synonyms:
  • EIICB-Glc
  • EII-Glc
  • Glucose permease IIC component
  • PTS system glucose-specific EIIC component
  • Glucose-specific phosphotransferase enzyme IIB component
  • PTS system glucose-specific EIIB component
Gene Name:ptsG
Enzyme Class:
Biological Properties
General Function:Involved in protein-N(PI)-phosphohistidine-sugar phosphotransferase activity
Specific Function:The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport. This enzyme is also a chemoreceptor monitoring the environment for changes in sugar concentration
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
  • Amino sugar and nucleotide sugar metabolism II PW000887
  • Amino sugar and nucleotide sugar metabolism III PW000895
  • Galactose metabolism PW000821
  • Starch and sucrose metabolism PW000941
  • colanic acid building blocks biosynthesis PW000951
  • galactose degradation/Leloir Pathway PW000884
  • glycerol metabolism PW000914
  • glycerol metabolism II PW000915
  • glycerol metabolism III (sn-glycero-3-phosphoethanolamine) PW000916
  • glycerol metabolism IV (glycerophosphoglycerol) PW000917
  • glycerol metabolism V (glycerophosphoserine) PW000918
  • glycolysis and pyruvate dehydrogenase PW000785
KEGG Pathways:
Transports:
Transport References:
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
KEGG Reactions:
1.0Protein N(pi)-phospho-L-histidine+1.0Thumb ↔ 1.0Protein histidine+1.0Thumb
1.0Protein N(pi)-phospho-L-histidine + 1.0D-Glucose ↔ 1.0Protein histidine + 1.0Glucose 6-phosphate
ReactionCard
1.0Protein N(pi)-phospho-L-histidine+1.0Sugar ↔ 1.0Protein histidine+1.0Thumb
1.0Protein N(pi)-phospho-L-histidine + 1.0Sugar ↔ 1.0Protein histidine + 1.0Sugar phosphate
ReactionCard
SMPDB Reactions:
1.0Glucosamine+1.0HPr - phosphorylated → 1.0Thumb+1.0HPr
1.0Glucosamine + 1.0HPr - phosphorylated → 1.0Glucosamine 6-phosphate + 1.0HPr
ReactionCard
1.0Thumb+1.0HPr - phosphorylated → 1.0Thumb+1.0HPr+1.0Thumb
1.0alpha-D-Glucose + 1.0HPr - phosphorylated → 1.0α-D-glucose 6-phosphate + 1.0HPr + 1.0Mannose 6-phosphate
ReactionCard
1.0Beta-D-Glucose+1.0HPr - phosphorylated+1.0Thumb → 1.0Thumb+1.0HPr
1.0Beta-D-Glucose + 1.0HPr - phosphorylated + 1.0b-D-Glucose → 1.0beta-D-Glucose 6-phosphate + 1.0HPr
ReactionCard
1.0Thumb+1.0[PTS enzyme I]-Nπ-phospho-L-histidine → 1.0Thumb+1.0[PTS enzyme I]-L-histidine
1.0D-Glucose + 1.0[PTS enzyme I]-Nπ-phospho-L-histidine → 1.0β-D-glucose 1-phosphate + 1.0[PTS enzyme I]-L-histidine
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Phosphoenolpyruvic acid + 1.0N-Acetyl-D-glucosamine → 1.0N-Acetyl-D-Glucosamine 6-Phosphate + 1.0Pyruvic acid
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Phosphoenolpyruvic acid + 1.0D-Glucose → 1.0Glucose 6-phosphate + 1.0Pyruvic acid
ReactionCard
1.0Protein EIIB N(pi)-phospho-L-histidine/cysteine+1.0Thumb → 1.0protein EIIB+1.0sugar phosphate
1.0Protein EIIB N(pi)-phospho-L-histidine/cysteine + 1.0Sucrose → 1.0protein EIIB + 1.0sugar phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB03345alpha-D-GlucoseMetaboCard
ECMDB23064Alpha-D-glucose 6-phosphateMetaboCard
ECMDB00516b-D-GlucoseMetaboCard
ECMDB03498beta-D-Glucose 6-phosphateMetaboCard
ECMDB00122D-GlucoseMetaboCard
ECMDB01254Glucosamine 6-phosphateMetaboCard
ECMDB01401Glucose 6-phosphateMetaboCard
ECMDB01078Mannose 6-phosphateMetaboCard
ECMDB00215N-Acetyl-D-glucosamineMetaboCard
ECMDB01062N-Acetyl-D-Glucosamine 6-PhosphateMetaboCard
ECMDB00263Phosphoenolpyruvic acidMetaboCard
ECMDB00243Pyruvic acidMetaboCard
ECMDB00258SucroseMetaboCard
ECMDB24239β-D-glucose 1-phosphateMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
Function
carbohydrate transmembrane transporter activity
cation transmembrane transporter activity
cation:sugar symporter activity
glucose transmembrane transporter activity
hexose transmembrane transporter activity
ion transmembrane transporter activity
monosaccharide transmembrane transporter activity
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity
solute:cation symporter activity
substrate-specific transmembrane transporter activity
sugar transmembrane transporter activity
sugar:hydrogen symporter activity
transmembrane transporter activity
transporter activity
Process
carbohydrate transport
establishment of localization
glucose transport
hexose transport
monosaccharide transport
phosphoenolpyruvate-dependent sugar phosphotransferase system
transport
Gene Properties
Blattner:b1101
Gene OrientationClockwise
Centisome Percentage:24.94
Left Sequence End1157092
Right Sequence End1158525
Gene Sequence:
>1434 bp
ATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCAC
GGCAGGCTGACGGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAG
CGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAA
TATGCAGAGCCGCTGGTCGAAAGCCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCA
AAACGGGTGTGGCGACGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCG
CAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACAGAACGACAGAACC
CGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCC
ACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGC
GGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTG
GAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGC
GGCCTGACGGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAA
ATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTTGTCAGCACGGAAGGG
GCAACAGAATGGTGCGCAGAATACGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCG
CATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTG
TATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGAGGTATATCACTCAGGATCCG
ATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGCTGAATCCGGTTCAGTATATA
GATTCAATGGGACTGGCATCAAAATATGGACACTTAAATAATGGCGGATATGGAGCGAGA
CCCAACAAACCGCCTACGCCCGATCCAAGTAAATTGCCGGACATAGCGAAACAATTAAGA
CTGCCATATCCTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGA
GCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACT
TGTACGCCACAGGATGATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTAC
CTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCC
TATTTATTCCCAGACATTAATAAACCCGATGGGCCAGCAACAGCAGGGATAGATGATTTG
GGTGAAATTTTAGCTAAGATGAAACAGAGAACATCGAGAGGAATAAGAAAATGA
Protein Properties
Pfam Domain Function:
Protein Residues:477
Protein Molecular Weight:50676
Protein Theoretical pI:9
PDB File:1O2F
Signaling Regions:
  • None
Transmembrane Regions:
  • 15-35
  • 51-71
  • 80-100
  • 112-132
  • 152-172
  • 191-211
  • 250-270
  • 280-300
  • 310-330
  • 356-376
Protein Sequence:
>PTS system glucose-specific EIICB component
MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVVSHVMAEAGGSVFANMP
LIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLHLPAEEIASKHLADTGVLG
GIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLAAIFTGVVLSFIWPPIGSAIQ
TFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPFQMQIGEYTNAAGQVFHGDIPRY
MAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPENRAKVGGIMISAALTSFLTGITEPI
EFSFMFVAPILYIIHAILAGLAFPICILLGMRDGTSFSHGLIDFIVLSGNSSKLWLFPIV
GIGYAIVYYTIFRVLIKALDLKTPGREDATEDAKATGTSEMAPALVAAFGGKENITNLDA
CITRLRVSVADVSKVDQAGLKKLGAAGVVVAGSGVQAIFGTKSDNLKTEMDEYIRNH
References
External Links:
ResourceLink
Uniprot ID:P69786
Uniprot Name:PTGCB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651310
PDB ID:1O2F
Ecogene ID:EG10787
Ecocyc:EG10787
ColiBase:b1101
Kegg Gene:b1101
EchoBASE ID:EB0780
CCDB:PTGCB_ECOLI
BacMap:16129064
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Eberstadt, M., Grdadolnik, S. G., Gemmecker, G., Kessler, H., Buhr, A., Erni, B. (1996). "Solution structure of the IIB domain of the glucose transporter of Escherichia coli." Biochemistry 35:11286-11292. Pubmed: 8784182
  • Erni, B., Zanolari, B. (1986). "Glucose-permease of the bacterial phosphotransferase system. Gene cloning, overproduction, and amino acid sequence of enzyme IIGlc." J Biol Chem 261:16398-16403. Pubmed: 3023349
  • Gemmecker, G., Eberstadt, M., Buhr, A., Lanz, R., Grdadolnik, S. G., Kessler, H., Erni, B. (1997). "Glucose transporter of Escherichia coli: NMR characterization of the phosphocysteine form of the IIB(Glc) domain and its binding interface with the IIA(Glc) subunit." Biochemistry 36:7408-7417. Pubmed: 9200688
  • Golic Grdadolnik, S., Eberstadt, M., Gemmecker, G., Kessler, H., Buhr, A., Erni, B. (1994). "The glucose transporter of Escherichia coli. Assignment of the 1H, 13C and 15N resonances and identification of the secondary structure of the soluble IIB domain." Eur J Biochem 219:945-952. Pubmed: 8112346
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Meins, M., Jeno, P., Muller, D., Richter, W. J., Rosenbusch, J. P., Erni, B. (1993). "Cysteine phosphorylation of the glucose transporter of Escherichia coli." J Biol Chem 268:11604-11609. Pubmed: 8505292
  • Morita, T., Mochizuki, Y., Aiba, H. (2006). "Translational repression is sufficient for gene silencing by bacterial small noncoding RNAs in the absence of mRNA destruction." Proc Natl Acad Sci U S A 103:4858-4863. Pubmed: 16549791
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Vanderpool, C. K., Gottesman, S. (2004). "Involvement of a novel transcriptional activator and small RNA in post-transcriptional regulation of the glucose phosphoenolpyruvate phosphotransferase system." Mol Microbiol 54:1076-1089. Pubmed: 15522088