Phosphomethylpyrimidine synthase (P30136)

Identification
Name:Phosphomethylpyrimidine synthase
Synonyms:
  • Hydroxymethylpyrimidine phosphate synthase
  • HMP-P synthase
  • HMP-phosphate synthase
  • HMPP synthase
  • Thiamine biosynthesis protein thiC
Gene Name:thiC
Enzyme Class:
Biological Properties
General Function:Involved in thiamine biosynthetic process
Specific Function:Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.04-Amino-5-hydroxymethyl-2-methylpyrimidine + 1.0S-Adenosylmethionine ↔ 1.05-Aminoimidazole ribonucleotide + 1.04-Amino-2-methyl-5-phosphomethylpyrimidine + 1.05'-Deoxyadenosine + 1.0L-Methionine + 1.0Formic acid + 1.0CO
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0S-adenosyl-L-methionine → 3.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb+1.04-amino-2-methyl-5-phosphomethylpyrimidine
1.05-Aminoimidazole ribonucleotide + 1.0S-adenosyl-L-methionine → 3.0Hydrogen ion + 1.0CO + 1.0Formic acid + 1.0L-Methionine + 1.05'-Deoxyadenosine + 1.04-amino-2-methyl-5-phosphomethylpyrimidine
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ? 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb+1.0carbon monoxide+1.0Thumb
1.05-Aminoimidazole ribonucleotide + 1.0S-Adenosylmethionine ? 1.04-Amino-2-methyl-5-phosphomethylpyrimidine + 1.05'-Deoxyadenosine + 1.0L-Methionine + 1.0Formic acid + 1.0carbon monoxide + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+2.0Thumb+3.0Thumb+1.0Thumb
1.05-Aminoimidazole ribonucleotide + 1.0Water + 1.0NAD → 1.04-Amino-2-methyl-5-phosphomethylpyrimidine + 2.0Formic acid + 3.0Hydrogen ion + 1.0NADH
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB200844-Amino-2-methyl-5-phosphomethylpyrimidineMetaboCard
ECMDB200864-Amino-5-hydroxymethyl-2-methylpyrimidineMetaboCard
ECMDB211745'-DeoxyadenosineMetaboCard
ECMDB012355-Aminoimidazole ribonucleotideMetaboCard
ECMDB23004COMetaboCard
ECMDB00142Formic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00696L-MethionineMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Process
cellular aromatic compound metabolic process
cellular metabolic process
metabolic process
thiamin and derivative metabolic process
thiamin biosynthetic process
thiamin metabolic process
Gene Properties
Blattner:b3994
Gene OrientationCounterclockwise
Centisome Percentage:90.36
Left Sequence End4192227
Right Sequence End4194122
Gene Sequence:
>1896 bp
ATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTA
AGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTT
GAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCG
CGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCT
GATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCT
GAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAA
CGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTC
CTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTT
GAAATTTATAAGACCCGTTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGC
GAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCTTGAG
GACGGAAGAATTGAATCCCGCGATCCGTTTTATCGTGAAGATTTCACGGTCCTCTACCGC
TGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCAT
CTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGTTCCTGGTTTGT
GGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACC
GATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTAGTTTAC
GACGATAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTG
GAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGA
AATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGC
GATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATG
CAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAA
TGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGG
GCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTA
TTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTA
ATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATC
AATGTCGGAGATGTATTCTTTTACTGGTATATGCCAGCGGGTTATTTTACCGCTGTTTGC
CTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGA
CAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCA
ACACACAGTGGACGTCGGCGGAAAGGGGAAACACGGAAACTGGATTACGCGTTGCTAGGT
TGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCT
TCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCAC
CTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGC
GGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:631
Protein Molecular Weight:70850
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Phosphomethylpyrimidine synthase
MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRVPMREIQLSPTLIGGSK
EQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKA
RLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITPEMEFIAIRENMGRERIRSEV
LRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
GNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQAL
EKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMA
KWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFAELHTLGELT
KIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
GAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAM
SKARFEFRWEDQFNLALDPFTARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDY
AATQTIEMGMADMSENFRARGGEIYLRKEEA
References
External Links:
ResourceLink
Uniprot ID:P30136
Uniprot Name:THIC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674891
Ecogene ID:EG11585
Ecocyc:EG11585
ColiBase:b3994
Kegg Gene:b3994
EchoBASE ID:EB1544
CCDB:THIC_ECOLI
BacMap:16131824
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Vander Horn, P. B., Backstrom, A. D., Stewart, V., Begley, T. P. (1993). "Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12." J Bacteriol 175:982-992. Pubmed: 8432721