Sulfate transport system permease protein cysT (P16701)

Identification
Name:Sulfate transport system permease protein cysT
Synonyms:Not Available
Gene Name:cysU
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Part of the ABC transporter complex cysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Sulfate+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Sulfate+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Sulfate + 1.0Sulfate → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0Sulfate + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Thiosulfate + 1.0Adenosine triphosphate + 1.0Water → 1.0ADP + 1.0Phosphate + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Molybdate → 1.0ADP + 1.0Hydrogen ion + 1.0Molybdate + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Sulfate → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Sulfate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Thiosulfate → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Thiosulfate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21246MolybdateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB21355SeleniteMetaboCard
ECMDB06343SelenocystathionineMetaboCard
ECMDB01448SulfateMetaboCard
ECMDB00257ThiosulfateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
Function
anion transmembrane transporter activity
inorganic anion transmembrane transporter activity
ion transmembrane transporter activity
substrate-specific transmembrane transporter activity
sulfate transmembrane transporter activity
transmembrane transporter activity
transporter activity
uptake transmembrane transporter activity
Process
anion transport
establishment of localization
inorganic anion transport
ion transport
sulfate transport
transport
Gene Properties
Blattner:b2424
Gene OrientationCounterclockwise
Centisome Percentage:54.74
Left Sequence End2539701
Right Sequence End2540534
Gene Sequence:
>834 bp
ATGCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAG
GATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTAC
AATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCAAGTCGTA
GTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGAAAAATC
CATCCTGGTGAATATTTTACTTTGATTGGTAATAGCCCCCGCGATATACGCTTGAAGATG
TGTGGTTATCAGGCTTATTTTAGTCGTACGGGGAGAAAGGAAATTCCTTCCGGCAATAGA
ACGAAACGAATATTGATAAATGTTCCAGGTATTTATAGTGACAGTTTTTGGGCGTCTATA
ATACGTGGAGAACTATCAGAGCTTTCACAGCCTACAGATGATGAATCGCTTCTGAATATG
AGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTT
GAGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCTTGGATTTTA
CAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTCCGTTTTATTTAAATGAT
GGAAACCCATATTTGGAAGTACATCATGTAATTCCCCTGTCTTCAGGTGGTGCTGATACA
ACAGATAACTGTGTTGCCCTTTGTCCGAATTGCCATAGAGAATTGCACTATAGTAAAAAT
GCAAAAGAACTAATCGAGATGCTTTACGTTAATATAAACCGATTACAGAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:277
Protein Molecular Weight:30292
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • 17-37
  • 64-84
  • 99-119
  • 136-156
  • 185-205
  • 215-235
  • 243-263
Protein Sequence:
>Sulfate transport system permease protein cysT
MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSWAQYWEVITNPQVVAAY
KVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDLPFALPTAVAGLTLASLFS
VNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQPVLEELGPEYEEAAETLGAT
RWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
DYPAASAIASVILAASLLLLFSINTLQSRFGRRVVGH
References
External Links:
ResourceLink
Uniprot ID:P16701
Uniprot Name:CYST_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651571
Ecogene ID:EG10197
Ecocyc:EG10197
ColiBase:b2424
Kegg Gene:b2424
EchoBASE ID:EB0194
CCDB:CYST_ECOLI
BacMap:16130349
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hryniewicz, M., Sirko, A., Palucha, A., Bock, A., Hulanicka, D. (1990). "Sulfate and thiosulfate transport in Escherichia coli K-12: identification of a gene encoding a novel protein involved in thiosulfate binding." J Bacteriol 172:3358-3366. Pubmed: 2188959
  • Sirko, A., Hryniewicz, M., Hulanicka, D., Bock, A. (1990). "Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster." J Bacteriol 172:3351-3357. Pubmed: 2188958
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837