Sulfate/thiosulfate import ATP-binding protein cysA (P16676)

Identification
Name:Sulfate/thiosulfate import ATP-binding protein cysA
Synonyms:
  • Sulfate-transporting ATPase
Gene Name:cysA
Enzyme Class:
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Part of the ABC transporter complex cysAWTP involved in sulfate/thiosulfate import. Responsible for energy coupling to the transport system
Cellular Location:Cell inner membrane; Peripheral membrane protein
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Sulfate+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Sulfate+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Sulfate + 1.0Sulfate → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0Sulfate + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Thiosulfate + 1.0Adenosine triphosphate + 1.0Water → 1.0ADP + 1.0Phosphate + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Molybdate → 1.0ADP + 1.0Hydrogen ion + 1.0Molybdate + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Sulfate → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Sulfate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Thiosulfate → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Thiosulfate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0sulfate(Out) → 1.0Thumb+1.0Thumb+1.0sulfate(In)
1.0Adenosine triphosphate + 1.0Water + 1.0sulfate(Out) → 1.0ADP + 1.0Inorganic phosphate + 1.0sulfate(In)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB21246MolybdateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB21355SeleniteMetaboCard
ECMDB06343SelenocystathionineMetaboCard
ECMDB01448SulfateMetaboCard
ECMDB00257ThiosulfateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
ATP-binding cassette (ABC) transporter complex
cell part
macromolecular complex
membrane
protein complex
Function
active transmembrane transporter activity
adenyl nucleotide binding
adenyl ribonucleotide binding
anion transmembrane transporter activity
ATP binding
ATPase activity
ATPase activity, coupled to transmembrane movement of substances
binding
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
inorganic anion transmembrane transporter activity
ion transmembrane transporter activity
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
P-P-bond-hydrolysis-driven transmembrane transporter activity
primary active transmembrane transporter activity
purine nucleoside binding
pyrophosphatase activity
substrate-specific transmembrane transporter activity
sulfate transmembrane transporter activity
sulfate transmembrane-transporting ATPase activity
transmembrane transporter activity
transporter activity
Process
anion transport
establishment of localization
inorganic anion transport
ion transport
sulfate transport
transport
Gene Properties
Blattner:b2422
Gene OrientationCounterclockwise
Centisome Percentage:54.70
Left Sequence End2537739
Right Sequence End2538836
Gene Sequence:
>1098 bp
GTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCACGACGGCGGCTCAGGCTGAG
CGTATTCGCGATCTCACCAGTGTTCAGGGGGTAAGGCAAAACTCACTGATTGGCTATGGT
CTGGTGGTGGGGCTGGATGGCACCGGTGACCAGACAACCCAGACGCCGTTTACCACACAA
ACGCTTAATAACATGCTCTCACAGCTGGGAATTACCGTTCCGACGGGCACCAATATGCAG
CTAAAAAACGTCGCTGCGGTAATGGTGACAGCGTCACTTCCTCCGTTTGGACGTCAGGGG
CAAACCATCGATGTGGTGGTTTCTTCCATGGGAAATGCCAAAAGCTTGCGTGGAGGTACG
TTGTTGATGACACCGCTTAAGGGCGTTGACAGTCAGGTGTATGCGCTGGCGCAGGGCAAT
ATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTG
AACGGTGGACGGATCACCAATGGTGCGGTTATTGAACGTGAATTGCCCAGCCAGTTTGGC
GTCGGGAATACCCTTAATTTGCAACTTAACGACGAAGATTTCAGCATGGCGCAGCAAATC
GCTGACACCATCAACCGCGTGCGTGGATATGGCAGCGCCACCGCGTTAGATGCGCGGACT
ATTCAGGTGCGCGTACCGAGTGGCAACAGTTCCCAGGTCCGCTTCCTTGCCGATATTCAG
AATATGCAGGTTAATGTCACCCCGCAGGACGCTAAAGTAGTGATTAACTCGCGCACCGGT
TCGGTGGTGATGAATCGCGAAGTGACCCTCGACAGCTGCGCGGTAGCGCAGGGGAATCTC
TCAGTAACAGTTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGA
CAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAA
AGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCT
ACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCA
AAACTGGAAATCATCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:365
Protein Molecular Weight:41059
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Sulfate/thiosulfate import ATP-binding protein cysA
MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTLLRIIAGLEHQTSGHIR
FHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVLPRRERPNAAAIKAKVTKL
LEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLLDEPFGALDAQVRKELRRWLR
QLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQADAPDQVWREPATRFVLEFMGEV
NRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLRPWEVDISRRTSLDSPLPVQVLEAS
PKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGERLFVGLQHARLYNGDERIETRDEELA
LAQSA
References
External Links:
ResourceLink
Uniprot ID:P16676
Uniprot Name:CYSA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062658
Ecogene ID:EG10183
Ecocyc:EG10183
ColiBase:b2422
Kegg Gene:b2422
EchoBASE ID:EB0180
CCDB:CYSA_ECOLI
BacMap:16130348
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Rosentel, J. K., Healy, F., Maupin-Furlow, J. A., Lee, J. H., Shanmugam, K. T. (1995). "Molybdate and regulation of mod (molybdate transport), fdhF, and hyc (formate hydrogenlyase) operons in Escherichia coli." J Bacteriol 177:4857-4864. Pubmed: 7665461
  • Sirko, A., Hryniewicz, M., Hulanicka, D., Bock, A. (1990). "Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster." J Bacteriol 172:3351-3357. Pubmed: 2188958
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837