ECMDB: Glutaredoxin-4 (P0AC69)

Identification

Name:

Glutaredoxin-4

Synonyms:

Grx4
Monothiol glutaredoxin

Gene Name:

grxD

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in electron carrier activity

Specific Function:

Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters (Probable)

Cellular Location:

Cytoplasm

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

Complex Reactions:

1.0glutaredoxin

1.0

→

1.0

1.0glutaredoxin

1.0

1.0glutaredoxin + 1.0Uridine 5'-diphosphate → 1.0dUDP + 1.0glutaredoxin + 1.0Water
ReactionCard

1.0

1.0glutaredoxin

→

1.0

1.0glutaredoxin

1.0

1.0Guanosine diphosphate + 1.0glutaredoxin → 1.0dGDP + 1.0glutaredoxin + 1.0Water
ReactionCard

1.0

1.0glutaredoxin

→

1.0

1.0glutaredoxin

1.0

1.0CDP + 1.0glutaredoxin → 1.0dCDP + 1.0glutaredoxin + 1.0Water
ReactionCard

1.0

1.0glutaredoxin

→

1.0

1.0glutaredoxin

1.0

1.0ADP + 1.0glutaredoxin → 1.0dADP + 1.0glutaredoxin + 1.0Water
ReactionCard

1.0glutaredoxin

1.0

→

1.0glutaredoxin

2.0

1.0

1.0glutaredoxin + 1.0Phosphoadenosine phosphosulfate → 1.0glutaredoxin + 2.0Hydrogen ion + 1.0Adenosine 3',5'-diphosphate + 1.0Sulfite
ReactionCard

1.0glutaredoxin

2.0

→

1.0glutaredoxin

1.0

1.0glutaredoxin + 2.0Glutathione → 1.0glutaredoxin + 1.0Glutathione disulfide
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00061	Adenosine 3',5'-diphosphate	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB01546	CDP	MetaboCard
ECMDB21326	dADP	MetaboCard
ECMDB01245	dCDP	MetaboCard
ECMDB00960	dGDP	MetaboCard
ECMDB01000	dUDP	MetaboCard
ECMDB00125	Glutathione	MetaboCard
ECMDB21221	Glutathione disulfide	MetaboCard
ECMDB01201	Guanosine diphosphate	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB01134	Phosphoadenosine phosphosulfate	MetaboCard
ECMDB00240	Sulfite	MetaboCard
ECMDB00295	Uridine 5'-diphosphate	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
disulfide oxidoreductase activity
electron carrier activity
oxidoreductase activity
oxidoreductase activity, acting on a sulfur group of donors
protein disulfide oxidoreductase activity
Process
cell redox homeostasis
cellular homeostasis
cellular process

Gene Properties

Blattner:

b1654

Gene Orientation

Counterclockwise

Centisome Percentage:

37.33

Left Sequence End

1731778

Right Sequence End

1732125

Gene Sequence:

>348 bp
ATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTA
AAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGT
GAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAAC
TGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGAT
CAGTATGGTACGACCGTTATCGCAGGGGAAGATGCGCAGAAAGATGTCGGTGAAGCGAAG
TACAACCCATTCGCTGACCTGAAAGCGATGATGAACAAGAAGAAGTGA

Protein Properties

Pfam Domain Function:

Glutaredoxin (PF00462 )

Protein Residues:

115

Protein Molecular Weight:

12879

Protein Theoretical pI:

PDB File:

1YKA

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Glutaredoxin-4
MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACGERFAYVDILQNPDIRA
ELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKETAAKYKSEEPDAE

References

External Links:

Resource	Link
Uniprot ID:	P0AC69
Uniprot Name:	GLRX4_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674550
PDB ID:	1YKA
Ecogene ID:	EG12181
Ecocyc:	EG12181
ColiBase:	b1654
Kegg Gene:	b1654
EchoBASE ID:	EB2098
CCDB:	GLRX4_ECOLI
BacMap:	16129612

General Reference:

Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Fernandes, A. P., Fladvad, M., Berndt, C., Andresen, C., Lillig, C. H., Neubauer, P., Sunnerhagen, M., Holmgren, A., Vlamis-Gardikas, A. (2005). "A novel monothiol glutaredoxin (Grx4) from Escherichia coli can serve as a substrate for thioredoxin reductase." J Biol Chem 280:24544-24552. Pubmed: 15833738
Fladvad, M., Bellanda, M., Fernandes, A. P., Mammi, S., Vlamis-Gardikas, A., Holmgren, A., Sunnerhagen, M. (2005). "Molecular mapping of functionalities in the solution structure of reduced Grx4, a monothiol glutaredoxin from Escherichia coli." J Biol Chem 280:24553-24561. Pubmed: 15840565
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Iwema, T., Picciocchi, A., Traore, D. A., Ferrer, J. L., Chauvat, F., Jacquamet, L. (2009). "Structural basis for delivery of the intact [Fe2S2] cluster by monothiol glutaredoxin." Biochemistry 48:6041-6043. Pubmed: 19505088
Reuven, N. B., Koonin, E. V., Rudd, K. E., Deutscher, M. P. (1995). "The gene for the longest known Escherichia coli protein is a member of helicase superfamily II." J Bacteriol 177:5393-5400. Pubmed: 7559321