Lactaldehyde reductase (P0A9S1)

Identification
Name:Lactaldehyde reductase
Synonyms:
  • Propanediol oxidoreductase
Gene Name:fucO
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity
Specific Function:(R)-propane-1,2-diol + NAD(+) = (R)- lactaldehyde + NADH
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Ethylene glycol + 1.0NAD ↔ 1.0Glycolaldehyde + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Propylene glycol + 1.0NAD ↔ 1.0Lactaldehyde + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0(R)-Propane-1,2-diol + 1.0(S)-Propane-1,2-diol + 1.0NAD ↔ 1.0D-Lactaldehyde + 1.0(S)-Lactaldehyde + 1.0NADH + 1.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.0(S)-lactaldehyde+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0(S)-lactaldehyde + 1.0NADH + 1.0Hydrogen ion + 1.0Lactaldehyde → 1.0NAD + 1.0Propylene glycol
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Propylene glycol + 1.0NAD ↔ 1.0Lactaldehyde + 1.0NADH + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Lactaldehyde + 1.0NADH ↔ 1.0(S)-Propane-1,2-diol + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0(R)-Propane-1,2-diol + 1.0NAD → 1.0D-Lactaldehyde + 1.0NADH
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0(S)-Propane-1,2-diol + 1.0NAD → 1.0Lactaldehyde + 1.0NADH
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21100(R)-Propane-1,2-diolMetaboCard
ECMDB23841(S)-LactaldehydeMetaboCard
ECMDB21102(S)-Propane-1,2-diolMetaboCard
ECMDB06458D-LactaldehydeMetaboCard
ECMDB20146Ethylene glycolMetaboCard
ECMDB02165GlycolaldehydeMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB03052LactaldehydeMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB01881Propylene glycolMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
ion binding
metal ion binding
oxidoreductase activity
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b2799
Gene OrientationCounterclockwise
Centisome Percentage:63.15
Left Sequence End2929887
Right Sequence End2931035
Gene Sequence:
>1152 bp
ATGTTTGAACCAATGGAACTTACCAATGACGCGGTGATTAAAGTCATCGGCGTCGGCGGC
GGCGGCGGTAATGCTGTTGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTC
GCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACAGACGATTCAAATC
GGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCG
GCTGATGAGGATCGCGATGCATTGCGTGCGGCGCTGGAAGGTGCAGACATGGTCTTTATT
GCTGCGGGTATGGGTGGTGGTACCGGTACAGGTGCAGCACCAGTCGTCGCTGAAGTGGCA
AAAGATTTGGGTATCCTGACCGTTGCTGTCGTCACTAAGCCTTTCAACTTTGAAGGCAAG
AAGCGTATGGCATTCGCGGAGCAGGGGATCACTGAACTGTCCAAGCATGTGGACTCTCTG
ATCACTATCCCGAACGACAAACTGCTGAAAGTTCTGGGCCGCGGTATCTCCCTGCTGGAT
GCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGATT
ACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTGAGATG
GGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGTGCGGAAGAAGCTGCT
GAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTG
CTGGTTAACATCACGGCGGGCTTCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAAC
ACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCG
GATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGT
CCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAG
CAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGAC
AATGCGCCGCAAACTGCGAAAGAGCCGGATTATCTGGATATCCCAGCATTCCTGCGTAAG
CAAGCTGATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:383
Protein Molecular Weight:40644
Protein Theoretical pI:5
PDB File:1RRM
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Lactaldehyde reductase
MMANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQCGVVAKVTDKMDAAGL
AWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDTCKAIGIISNNPEFADVR
SLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRRKFVCVDPHDIPQVAFIDAD
MMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHIKAIEIIAGALRGSVAGDKDAG
EEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPHGVANAILLPHVMRYNADFTGEKY
RDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVGIPPHLRDVGVRKEDIPALAQAALDD
VCTGGNPREATLEDIVELYHTAW
References
External Links:
ResourceLink
Uniprot ID:P0A9S1
Uniprot Name:FUCO_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674323
PDB ID:1RRM
Ecogene ID:EG10351
Ecocyc:EG10351
ColiBase:b2799
Kegg Gene:b2799
EchoBASE ID:EB0347
CCDB:FUCO_ECOLI
BacMap:16130706
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Chen, Y. M., Lu, Z., Lin, E. C. (1989). "Constitutive activation of the fucAO operon and silencing of the divergently transcribed fucPIK operon by an IS5 element in Escherichia coli mutants selected for growth on L-1,2-propanediol." J Bacteriol 171:6097-6105. Pubmed: 2553671
  • Conway, T., Ingram, L. O. (1989). "Similarity of Escherichia coli propanediol oxidoreductase (fucO product) and an unusual alcohol dehydrogenase from Zymomonas mobilis and Saccharomyces cerevisiae." J Bacteriol 171:3754-3759. Pubmed: 2661535
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lu, Z., Lin, E. C. (1989). "The nucleotide sequence of Escherichia coli genes for L-fucose dissimilation." Nucleic Acids Res 17:4883-4884. Pubmed: 2664711
  • Shao, Z., Newman, E. B. (1993). "Sequencing and characterization of the sdaB gene from Escherichia coli K-12." Eur J Biochem 212:777-784. Pubmed: 8385012