ECMDB

Identification

Name:

Phenylacetaldehyde dehydrogenase

Synonyms:

Gene Name:

feaB

Enzyme Class:

1.2.1.39

Biological Properties

General Function:

Involved in oxidoreductase activity

Specific Function:

Acts almost equally well on phenylacetaldehyde, 4- hydroxyphenylacetaldehyde and 3,4-dihydroxyphenylacetaldehyde

Cellular Location:

Not Available

SMPDB Pathways:

Phenylalanine metabolism PW000921
Phenylethylamine metabolism PW002027

KEGG Pathways:

Metabolic pathways eco01100
Microbial metabolism in diverse environments ec01120
Phenylalanine metabolism ec00360
Styrene degradation ec00643

KEGG Reactions:

1.0

↔

2.0

1.0

1.0Water + 1.0NAD + 1.0Phenylacetaldehyde ↔ 2.0Hydrogen ion + 1.0NADH + 1.0Benzeneacetic acid
ReactionCard

1.0

↔

1.0

1.0Phenylacetaldehyde + 1.0NAD + 1.0Water ↔ 1.0Benzeneacetic acid + 1.0NADH + 1.0Hydrogen ion
ReactionCard

SMPDB Reactions:

1.0

→

1.0

1.0Phenylacetaldehyde + 1.0NAD + 1.0Water → 1.0NADH + 1.0Hydrogen ion + 1.0Benzeneacetic acid
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Phenylacetaldehyde + 1.0NAD + 1.0Water → 1.0Benzeneacetic acid + 1.0NADH
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB04128	Benzeneacetic acid	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00902	NAD	MetaboCard
ECMDB01487	NADH	MetaboCard
ECMDB06236	Phenylacetaldehyde	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
oxidoreductase activity
Process
metabolic process
oxidation reduction

Gene Properties

Blattner:

b1385

Gene Orientation

Clockwise

Centisome Percentage:

31.16

Left Sequence End

1445543

Right Sequence End

1447042

Gene Sequence:

>1500 bp
ATGACAGAGCCGCATGTAGCAGTATTAAGCCAGGTCCAACAGTTTCTCGATCGTCAACAC
GGTCTTTATATTGATGGTCGTCCTGGCCCCGCACAAAGTGAAAAACGGTTGGCGATCTTT
GATCCGGCCACCGGGCAAGAAATTGCGTCTACTGCTGATGCCAACGAAGCGGATGTAGAT
AACGCAGTCATGTCTGCCTGGCGGGCCTTTGTCTCGCGTCGCTGGGCCGGGCGATTACCC
GCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAG
CTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAA
GTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGT
AAAACGCTGGACTTGTCGATTCCCTTACCCCAGGGGGCGCGTTATCAGGCCTGGACGCGT
AAAGAGCCGGTTGGCGTAGTGGCGGGAATTGTGCCATGGAACTTTCCGTTGATGATTGGT
ATGTGGAAGGTGATGCCAGCACTGGCAGCAGGCTGTTCAATCGTGATTAAGCCTTCGGAA
ACCACGCCACTGACGATGTTGCGCGTGGCGGAACTGGCCAGCGAGGCTGGTATCCCTGAT
GGCGTTTTTAATGTCGTCACCGGGTCAGGTGCTGTATGCGGCGCGGCCCTGACGTCACAT
CCTCATGTTGCGAAAATCAGTTTTACCGGTTCAACCGCGACGGGAAAAGGTATTGCCAGA
ACTGCTGCTGATCACTTAACGCGTGTAACGCTGGAACTGGGCGGTAAAAACCCGGCAATT
GTATTAAAAGATGCTGATCCGCAATGGGTTATTGAAGGCTTGATGACCGGAAGCTTCCTG
AATCAAGGGCAAGTATGCGCCGCCAGTTCGCGAATTTATATTGAAGCGCCGTTGTTTGAC
ACGCTGGTTAGTGGATTTGAGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCA
CCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCACTGCGACAAAGTGTGTTCATTC
CTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGCCGGA
GAGGGGTATTATGTTGCGCCAACGCTGGTGGTAAATCCCGATGCTAAATTGCGCTTAACT
CGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCG
TTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGTCTGGACGCAAAATCTC
TCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCAT
ACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGAT
TTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAA

Protein Properties

Pfam Domain Function:

Aldedh (PF00171 )

Protein Residues:

499

Protein Molecular Weight:

53699

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Phenylacetaldehyde dehydrogenase
MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPATGQEIASTADANEADVD
NAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELAQLETLEQGKSIAISRAFE
VGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTRKEPVGVVAGIVPWNFPLMIG
MWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGIPDGVFNVVTGSGAVCGAALTSH
PHVAKISFTGSTATGKGIARTAADHLTRVTLELGGKNPAIVLKDADPQWVIEGLMTGSFL
NQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQVGPGMSPVAQINPLVSRAHCDKVCSF
LDDAQAQQAELIRGSNGPAGEGYYVAPTLVVNPDAKLRLTREEVFGPVVNLVRVADGEEA
LQLANDTEYGLTASVWTQNLSQALEYSDRLQAGTVWVNSHTLIDANLPFGGMKQSGTGRD
FGPDWLDGWCETKSVCVRY

References

External Links:

Resource	Link
Uniprot ID:	P80668
Uniprot Name:	FEAB_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674936
Ecogene ID:	EG13179
Ecocyc:	EG13179
ColiBase:	b1385
Kegg Gene:	b1385
EchoBASE ID:	EB2971
CCDB:	FEAB_ECOLI
BacMap:	90111264

General Reference:

Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Ferrandez, A., Prieto, M. A., Garcia, J. L., Diaz, E. (1997). "Molecular characterization of PadA, a phenylacetaldehyde dehydrogenase from Escherichia coli." FEBS Lett 406:23-27. Pubmed: 9109378
Hanlon, S. P., Hill, T. K., Flavell, M. A., Stringfellow, J. M., Cooper, R. A. (1997). "2-phenylethylamine catabolism by Escherichia coli K-12: gene organization and expression." Microbiology 143 ( Pt 2):513-518. Pubmed: 9043126
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553

Phenylacetaldehyde dehydrogenase (P80668)