2-hydroxy-3-oxopropionate reductase_ (P77161)

Identification
Name:2-hydroxy-3-oxopropionate reductase_
Synonyms:
  • Tartronate semialdehyde reductase
  • TSAR
Gene Name:glxR
Enzyme Class:
Biological Properties
General Function:Involved in 3-hydroxyisobutyrate dehydrogenase activity
Specific Function:(R)-glycerate + NAD(P)(+) = 2-hydroxy-3- oxopropanoate + NAD(P)H
Cellular Location:Not Available
SMPDB Pathways:
  • glycolate and glyoxylate degradation PW000827
  • glycolate and glyoxylate degradation II PW002021
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Tartronate semialdehyde + 1.0Hydrogen ion + 1.0NADH ↔ 1.0Glyceric acid + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glyceric acid + 1.0NAD ↔ 1.0Tartronate semialdehyde + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glyceric acid + 1.0NADP ↔ 1.0Tartronate semialdehyde + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Glyceric acid + 1.0NAD + 1.0NADP ↔ 1.0Tartronate semialdehyde + 1.0NADH + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Tartronate semialdehyde + 1.0NADH + 1.0Hydrogen ion → 1.0NAD + 1.0Glyceric acid
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Tartronate semialdehyde + 1.0Hydrogen ion + 1.0NADH ↔ 1.0Glyceric acid + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glyceric acid + 1.0NAD ↔ 1.0Tartronate semialdehyde + 1.0NADH + 1.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04077Glyceric acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
ECMDB06938Tartronate semialdehydeMetaboCard
GO Classification:
Function
2-hydroxy-3-oxopropionate reductase activity
3-hydroxyisobutyrate dehydrogenase activity
binding
catalytic activity
coenzyme binding
cofactor binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
phosphogluconate dehydrogenase (decarboxylating) activity
Process
alcohol metabolic process
branched chain family amino acid metabolic process
cellular aldehyde metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
glucose catabolic process
glucose metabolic process
glyoxylate metabolic process
hexose metabolic process
metabolic process
monosaccharide metabolic process
oxidation reduction
pentose-phosphate shunt
small molecule metabolic process
valine metabolic process
Gene Properties
Blattner:b0509
Gene OrientationClockwise
Centisome Percentage:11.55
Left Sequence End535810
Right Sequence End536688
Gene Sequence:
>879 bp
ATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTG
GCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTG
GGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAG
GGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCAT
CACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCG
CGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTT
CGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCC
GGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTA
CTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGC
GAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTC
GCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGG
ATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGC
ACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAT
TTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGT
GAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:292
Protein Molecular Weight:30800
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2-hydroxy-3-oxopropionate reductase
MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAVSVETARQVTEASDIIF
IMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVS
GGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVGGNGDGQTCKVANQIIVALNI
EAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPGFKIALHQKDLN
LALQSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALELMANHKLA
References
External Links:
ResourceLink
Uniprot ID:P77161
Uniprot Name:GLXR_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321946
Ecogene ID:EG13265
Ecocyc:EG13265
ColiBase:b0509
Kegg Gene:b0509
EchoBASE ID:EB3052
CCDB:GLXR_ECOLI
BacMap:16128493
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cusa, E., Obradors, N., Baldoma, L., Badia, J., Aguilar, J. (1999). "Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli." J Bacteriol 181:7479-7484. Pubmed: 10601204
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553