Low specificity L-threonine aldolase (P75823)

Identification
Name:Low specificity L-threonine aldolase
Synonyms:
  • Low specificity L-TA
Gene Name:ltaE
Enzyme Class:
Biological Properties
General Function:Involved in lyase activity
Specific Function:Catalyzes the cleavage of L-allo-threonine and L- threonine to glycine and acetaldehyde. L-threo-phenylserine and L- erythro-phenylserine are also good substrates
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:
  • Glycine, serine and threonine metabolism ec00260
  • Metabolic pathways eco01100
  • Microbial metabolism in diverse environments ec01120
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0L-Threonine ↔ 1.0Acetaldehyde + 1.0Glycine
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.0L-Allothreonine → 1.0Acetaldehyde + 1.0Glycine
ReactionCard
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0L-Allothreonine ↔ 1.0Glycine + 1.0Acetaldehyde
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0L-Threonine ↔ 1.0Acetaldehyde + 1.0Glycine
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0D-Alanine + 1.0Pyridoxal 5'-phosphate → 1.0Pyridoxamine 5'-phosphate + 1.0Pyruvic acid
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.0L-Allothreonine → 1.0Acetaldehyde + 1.0Glycine
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0L-Alanine + 1.0Pyridoxal 5'-phosphate → 1.0Pyridoxamine 5'-phosphate + 1.0Pyruvic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00990AcetaldehydeMetaboCard
ECMDB01310D-AlanineMetaboCard
ECMDB00123GlycineMetaboCard
ECMDB00161L-AlanineMetaboCard
ECMDB21007L-AllothreonineMetaboCard
ECMDB00167L-ThreonineMetaboCard
ECMDB01491Pyridoxal 5'-phosphateMetaboCard
ECMDB01555Pyridoxamine 5'-phosphateMetaboCard
ECMDB00243Pyruvic acidMetaboCard
GO Classification:
Function
binding
catalytic activity
cofactor binding
lyase activity
pyridoxal phosphate binding
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process
Gene Properties
Blattner:b0870
Gene OrientationCounterclockwise
Centisome Percentage:19.56
Left Sequence End907516
Right Sequence End908517
Gene Sequence:
>1002 bp
ATGATTGATTTACGCAGTGATACCGTTACCCGACCAAGCCGCGCCATGCTCGAAGCGATG
ATGGCCGCCCCGGTTGGGGACGACGTTTACGGAGACGACCCTACCGTTAATGCTCTGCAG
GACTACGCAGCAGAGCTTTCCGGTAAAGAAGCCGCCATTTTTCTGCCTACCGGCACTCAG
GCCAACCTGGTCGCTCTGCTCAGTCACTGCGAACGCGGCGAAGAGTATATTGTCGGTCAG
GCCGCGCATAACTATCTGTTTGAAGCCGGTGGCGCGGCGGTGCTGGGCAGTATTCAACCG
CAACCCATAGACGCGGCTGCCGACGGCACGCTACCGCTGGATAAAGTGGCGATGAAAATC
AAACCCGACGATATCCATTTCGCCCGCACCAAATTACTCAGTCTGGAAAACACCCACAAC
GGCAAAGTGTTGCCGCGGGAATACCTGAAAGAAGCATGGGAATTTACCCGCGAGCGCAAT
CTGGCGCTGCATGTTGACGGTGCGCGCATCTTTAATGCCGTGGTGGCTTACGGCTGCGAA
CTGAAAGAGATCACGCAATATTGTGATTCGTTCACCATTTGCCTGTCGAAAGGTCTTGGG
ACGCCAGTCGGTTCATTACTCGTCGGTAATCGTGATTACATTAAACGTGCCATTCGCTGG
CGGAAAATGACAGGTGGCGGGATGCGCCAGTCCGGCATTCTGGCTGCCGCCGGGATATAT
GCCCTGAAAAATAACGTTGCGCGCTTGCAGGAAGACCACGACAACGCTGCCTGGATGGCG
GAGCAGCTGCGTGAAGCAGGCGCGGATGTGATGCGTCAGGACACCAATATGCTGTTTGTT
CGCGTCGGGGAAGAAAATGCTGCCGCGTTAGGCGAATACATGAAAGCGAGAAACGTGCTG
ATTAACGCCTCGCCGATTGTCCGCCTGGTGACGCATCTTGACGTCTCGCGCGAACAACTG
GCGGAAGTCGCCGCCCACTGGCGTGCATTCCTGGCGCGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:333
Protein Molecular Weight:36494
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Low specificity L-threonine aldolase
MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAAELSGKEAAIFLPTGTQ
ANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQPIDAAADGTLPLDKVAMKI
KPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERNLALHVDGARIFNAVVAYGCE
LKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAIRWRKMTGGGMRQSGILAAAGIY
ALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTNMLFVRVGEENAAALGEYMKARNVL
INASPIVRLVTHLDVSREQLAEVAAHWRAFLAR
References
External Links:
ResourceLink
Uniprot ID:P75823
Uniprot Name:LTAE_ECOLI
GenBank Gene ID:AB005050
Genebank Protein ID:2225892
Ecogene ID:EG13690
Ecocyc:EG13690
ColiBase:b0870
Kegg Gene:b0870
EchoBASE ID:EB3454
CCDB:LTAE_ECOLI
BacMap:16128838
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Liu, J. Q., Dairi, T., Itoh, N., Kataoka, M., Shimizu, S., Yamada, H. (1998). "Gene cloning, biochemical characterization and physiological role of a thermostable low-specificity L-threonine aldolase from Escherichia coli." Eur J Biochem 255:220-226. Pubmed: 9692922
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232