Pyridoxine kinase (P40191)

Identification
Name:Pyridoxine kinase
Synonyms:
  • PN/PL/PM kinase
  • Pyridoxal kinase
  • Pyridoxamine kinase
  • Vitamin B6 kinase
Gene Name:pdxK
Enzyme Class:
Biological Properties
General Function:Involved in pyridoxal kinase activity
Specific Function:Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Pyridoxal ↔ 1.0ADP + 1.0Hydrogen ion + 1.0Pyridoxal 5'-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.04-Amino-5-hydroxymethyl-2-methylpyrimidine + 1.0Adenosine triphosphate ↔ 1.04-Amino-2-methyl-5-phosphomethylpyrimidine + 1.0ADP + 1.0Hydrogen ion + 1.04-amino-5-phosphonooxymethyl-2-methylpyrimidine
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Pyridoxine → 1.0ADP + 1.0Hydrogen ion + 1.0Pyridoxine 5'-phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Pyridoxamine → 1.0ADP + 1.0Hydrogen ion + 1.0Pyridoxamine 5'-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Pyridoxal ↔ 1.0ADP + 1.0Pyridoxal 5'-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Pyridoxine ↔ 1.0ADP + 1.0Pyridoxine 5'-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Pyridoxamine ↔ 1.0ADP + 1.0Pyridoxamine 5'-phosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb
1.0Pyridoxine + 1.0Adenosine triphosphate → 1.0Pyridoxine 5'-phosphate + 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Adenosine diphosphate+1.0Thumb
1.0Pyridoxal + 1.0Adenosine triphosphate → 1.0Pyridoxal 5'-phosphate + 1.0Adenosine diphosphate + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Adenosine diphosphate+1.0Thumb
1.0Pyridoxamine + 1.0Adenosine triphosphate → 1.0Pyridoxamine 5'-phosphate + 1.0Adenosine diphosphate + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Pyridoxal ↔ 1.0ADP + 1.0Hydrogen ion + 1.0Pyridoxal 5'-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.04-Amino-5-hydroxymethyl-2-methylpyrimidine + 1.0Adenosine triphosphate ↔ 1.04-Amino-2-methyl-5-phosphomethylpyrimidine + 1.0ADP + 1.0Hydrogen ion + 1.04-amino-5-phosphonooxymethyl-2-methylpyrimidine
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Pyridoxine → 1.0ADP + 1.0Hydrogen ion + 1.0Pyridoxine 5'-phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Pyridoxamine → 1.0ADP + 1.0Hydrogen ion + 1.0Pyridoxamine 5'-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Pyridoxamine ↔ 1.0ADP + 1.0Pyridoxamine 5'-phosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Pyridoxal → 1.0ADP + 1.0Pyridoxal 5'-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB200844-Amino-2-methyl-5-phosphomethylpyrimidineMetaboCard
ECMDB200864-Amino-5-hydroxymethyl-2-methylpyrimidineMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01545PyridoxalMetaboCard
ECMDB01491Pyridoxal 5'-phosphateMetaboCard
ECMDB01431PyridoxamineMetaboCard
ECMDB01555Pyridoxamine 5'-phosphateMetaboCard
ECMDB00239PyridoxineMetaboCard
ECMDB01319Pyridoxine 5'-phosphateMetaboCard
GO Classification:
Function
catalytic activity
kinase activity
pyridoxal kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
metabolic process
pyridoxine biosynthetic process
pyridoxine metabolic process
small molecule metabolic process
vitamin B6 metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process
Gene Properties
Blattner:b2418
Gene OrientationCounterclockwise
Centisome Percentage:54.62
Left Sequence End2534408
Right Sequence End2535259
Gene Sequence:
>852 bp
ATGGCTGAAATTACCGCATCCCTGGTAAAAGAGCTGCGTGAGCGTACTGGCGCAGGCATG
ATGGATTGCAAAAAAGCACTGACTGAAGCTAACGGCGACATCGAGCTGGCAATCGAAAAC
ATGCGTAAGTCCGGTGCTATTAAAGCAGCGAAAAAAGCAGGCAACGTTGCTGCTGACGGC
GTGATCAAAACCAAAATCGACGGCAACTACGGCATCATTCTGGAAGTTAACTGCCAGACT
GACTTCGTTGCAAAAGACGCTGGTTTCCAGGCGTTCGCAGACAAAGTTCTGGACGCAGCT
GTTGCTGGCAAAATCACTGACGTTGAAGTTCTGAAAGCACAGTTCGAAGAAGAACGTGTT
GCGCTGGTAGCGAAAATTGGTGAAAACATCAACATTCGCCGCGTTGCTGCGCTGGAAGGC
GACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGC
GCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTC
ATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGAT
ATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATG
AAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAA
ACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAA
GTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCC
AAGCAGTCTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:283
Protein Molecular Weight:30847
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Pyridoxine kinase
MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGLNVFAVPTVLLSNTPHY
DTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQIKILAEWLTALRKDHPDL
LIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNIFELEILTGKNCRDLDSAIAA
AKSLLSDTLKWVVVTSASGNEENQEMQVVVVTADSVNVISHSRVKTDLKGTGDLFCAQLI
SGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELILPPLAEA
References
External Links:
ResourceLink
Uniprot ID:P40191
Uniprot Name:PDXK_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21239025
Ecogene ID:EG12642
Ecocyc:EG12642
ColiBase:b2418
Kegg Gene:b2418
EchoBASE ID:EB2519
CCDB:PDXK_ECOLI
BacMap:16130344
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • De Reuse, H., Danchin, A. (1988). "The ptsH, ptsI, and crr genes of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system: a complex operon with several modes of transcription." J Bacteriol 170:3827-3837. Pubmed: 2457575
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Saffen, D. W., Presper, K. A., Doering, T. L., Roseman, S. (1987). "Sugar transport by the bacterial phosphotransferase system. Molecular cloning and structural analysis of the Escherichia coli ptsH, ptsI, and crr genes." J Biol Chem 262:16241-16253. Pubmed: 2960675
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837
  • Yang, Y., Zhao, G., Winkler, M. E. (1996). "Identification of the pdxK gene that encodes pyridoxine (vitamin B6) kinase in Escherichia coli K-12." FEMS Microbiol Lett 141:89-95. Pubmed: 8764513