Cytochrome c-type protein torC (P33226)

Identification
Name:Cytochrome c-type protein torC
Synonyms:Not Available
Gene Name:torC
Enzyme Class:Not Available
Biological Properties
General Function:Involved in iron ion binding
Specific Function:Part of the anaerobic respiratory chain of trimethylamine-N-oxide reductase torA. Acts by transferring electrons from the membranous menaquinones to torA. This transfer probably involves an electron transfer pathway from menaquinones to the N-terminal domain of torC, then from the N-terminus to the C-terminus, and finally to torA. TorC apocytochrome negatively autoregulates the torCAD operon probably by inhibiting the torS kinase activity
Cellular Location:Cell inner membrane; Single-pass type II membrane protein
SMPDB Pathways:
KEGG Pathways:
SMPDB Reactions:
1.0Thumb+3.0Thumb+1.0Thumb+2.0Electron → 1.0Thumb+1.0Thumb+2.0Thumb+1.0menaquinone-8
1.0Trimethylamine N-Oxide + 3.0Hydrogen ion + 1.0Menaquinol 8 + 2.0Electron → 1.0Trimethylamine + 1.0Water + 2.0Hydrogen ion + 1.0menaquinone-8
ReactionCard
1.0Thumb+1.0Thumb+2.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Trimethylamine N-Oxide + 1.0NADH + 2.0Hydrogen ion → 1.0Trimethylamine + 1.0NAD + 1.0Water
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Menaquinone 8+1.0Thumb
1.0Hydrogen ion + 1.0Menaquinol 8 + 1.0Trimethylamine N-Oxide → 1.0Water + 1.0Menaquinone 8 + 1.0Trimethylamine
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.02-Demethylmenaquinol 8 + 1.0Hydrogen ion + 1.0Trimethylamine N-Oxide → 1.02-Demethylmenaquinone 8 + 1.0Water + 1.0Trimethylamine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211542-Demethylmenaquinol 8MetaboCard
ECMDB211552-Demethylmenaquinone 8MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00906TrimethylamineMetaboCard
ECMDB04161Trimethylamine N-OxideMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
cell wall
external encapsulating structure
Gram-negative-bacterium-type cell wall
integral to membrane
intrinsic to membrane
membrane part
peptidoglycan-based cell wall
Function
binding
cation binding
electron carrier activity
heme binding
ion binding
iron ion binding
metal ion binding
transition metal ion binding
Gene Properties
Blattner:b0996
Gene OrientationClockwise
Centisome Percentage:22.79
Left Sequence End1057307
Right Sequence End1058479
Gene Sequence:
>1173 bp
ATGCGGAAACTCTGGAACGCGCTACGCCGACCCAGTGCTCGTTGGTCGGTACTGGCGCTG
GTCGCAATTGGGATTGTGATTGGCATTGCGCTGATTGTATTGCCACACGTTGGGATCAAA
GTCACCAGCACAACCGAATTTTGTGTCAGTTGCCACAGTATGCAACCGGTGTATGAAGAA
TATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGT
CATATCCCGCCGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAGCAATGATATC
TACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCGCG
GAACTTGCCGAGCGTGAATGGGCGCGAATGAAAGAAAACAACTCGGCAACCTGCCGCTCC
TGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATG
AAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAG
TTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAAT
GACAGTGGTGACACGCTGTACTCTATTGATATTAAGCCAATTTATGCGGCGAAAGGCGAT
AAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGAAGTGAAAGTCCTTAAACGTGACGGC
GACTGGCTGCAAATTGAAATTACCGGCTGGACGGAAAGCGCCGGACGTCAGCGTGTACTC
ACCCAATTCCCAGGTAAACGCATCTTTGTTGCCTCGATTCGTGGTGATGTGCAGCAGCAG
GTAAAAACGCTGGAGAAAACCACCGTTGCCGACACCAATACCGAGTGGAGCAAGTTGCAG
GCCACTGCGTGGATGAAGAAAGGCGACATGGTGAACGATATCAAACCGATCTGGGCTTAT
GCGGATTCGTTGTACAACGGCACCTGTAACCAGTGCCACGGCGCACCGGAAATCGCCCAC
TTTGACGCTAACGGTTGGATCGGCACGCTCAACGGCATGATTGGCTTTACCAGTCTCGAT
AAACGTGAAGAACGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGT
AAGGCTCACGGCGATAAGAAGGAAGAAAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:390
Protein Molecular Weight:43606
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • 17-37
Protein Sequence:
>Cytochrome c-type protein torC
MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTSTTEFCVSCHSMQPVYEE
YKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQTFIAHSIDTPEKFEAKRA
ELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQMKVAAKDNQSCIDCHKGIAHQ
LPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAKGDKEASGSLLPASEVKVLKRDG
DWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGDVQQQVKTLEKTTVADTNTEWSKLQ
ATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHGAPEIAHFDANGWIGTLNGMIGFTSLD
KREERTLLKYLQMNASDTAGKAHGDKKEEK
References
External Links:
ResourceLink
Uniprot ID:P33226
Uniprot Name:TORC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651488
Ecogene ID:EG11815
Ecocyc:EG11815
ColiBase:b0996
Kegg Gene:b0996
EchoBASE ID:EB1762
CCDB:TORC_ECOLI
BacMap:16128962
General Reference:
  • Ansaldi, M., Bordi, C., Lepelletier, M., Mejean, V. (1999). "TorC apocytochrome negatively autoregulates the trimethylamine N-oxide (TMAO) reductase operon in Escherichia coli." Mol Microbiol 33:284-295. Pubmed: 10411745
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Gon, S., Giudici-Orticoni, M. T., Mejean, V., Iobbi-Nivol, C. (2001). "Electron transfer and binding of the c-type cytochrome TorC to the trimethylamine N-oxide reductase in Escherichia coli." J Biol Chem 276:11545-11551. Pubmed: 11056172
  • Gon, S., Jourlin-Castelli, C., Theraulaz, L., Mejean, V. (2001). "An unsuspected autoregulatory pathway involving apocytochrome TorC and sensor TorS in Escherichia coli." Proc Natl Acad Sci U S A 98:11615-11620. Pubmed: 11562502
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Iobbi-Nivol, C., Crooke, H., Griffiths, L., Grove, J., Hussain, H., Pommier, J., Mejean, V., Cole, J. A. (1994). "A reassessment of the range of c-type cytochromes synthesized by Escherichia coli K-12." FEMS Microbiol Lett 119:89-94. Pubmed: 8039676
  • Mejean, V., Iobbi-Nivol, C., Lepelletier, M., Giordano, G., Chippaux, M., Pascal, M. C. (1994). "TMAO anaerobic respiration in Escherichia coli: involvement of the tor operon." Mol Microbiol 11:1169-1179. Pubmed: 8022286
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232