ECMDB: Probable nitrate reductase molybdenum cofactor assembly chaperone NarW (P19317)

Identification

Name:

Probable nitrate reductase molybdenum cofactor assembly chaperone NarW

Synonyms:

Redox enzyme maturation protein NarW

Gene Name:

narW

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in unfolded protein binding

Specific Function:

Chaperone required for proper molybdenum cofactor insertion and final assembly of the membrane-bound respiratory nitrate reductase 2

Cellular Location:

Cytoplasm

SMPDB Pathways:

Nitrogen metabolism PW000755
Selenium metabolism PW001894
nitrate reduction VIII PW002092

KEGG Pathways:

Nitrogen metabolism ec00910

KEGG Reactions:

1.0

1.0Acceptor

1.0

1.0Acceptor

↔

1.0

1.0Reduced acceptor

1.0Nitrite + 1.0Acceptor + 1.0Water + 1.0Acceptor ↔ 1.0Nitrate + 1.0Reduced acceptor + 1.0Reduced acceptor
ReactionCard

2.0Ferricytochrome c

1.0

↔

1.0

2.0Ferrocytochrome c

2.0

2.0Ferricytochrome c + 1.0Nitrite + 1.0Water ↔ 1.0Nitrate + 2.0Ferrocytochrome c + 2.0Hydrogen ion
ReactionCard

SMPDB Reactions:

1.0Nitrate

1.0cytochrome c nitrite reductase

1.0

↔

1.0Nitrite

1.0cytochrome c nitrite reductase

1.0

1.0Nitrate + 1.0cytochrome c nitrite reductase + 1.0Nitrate ↔ 1.0Nitrite + 1.0cytochrome c nitrite reductase + 1.0Water + 1.0Nitrite
ReactionCard

1.0

2.0

2.0Electron

→

1.0

1.0Selenate + 2.0Hydrogen ion + 2.0Electron → 1.0Selenite + 1.0Water
ReactionCard

1.0

2.0

1.0a menaquinol

→

1.0

1.0a menaquinone

1.0Nitrate + 2.0Hydrogen ion + 1.0a menaquinol → 1.0Nitrite + 1.0Water + 1.0a menaquinone
ReactionCard

Complex Reactions:

2.0

1.0

→

1.0

2.0

2.0Hydrogen ion + 1.0Nitrate + 1.0Ubiquinol-8 → 1.0Water + 1.0Nitrite + 1.0Ubiquinone-8 + 2.0Hydrogen ion
ReactionCard

2.0

1.0

→

1.0

1.0Menaquinone 8

1.0

2.0

2.0Hydrogen ion + 1.0Menaquinol 8 + 1.0Nitrate → 1.0Water + 1.0Menaquinone 8 + 1.0Nitrite + 2.0Hydrogen ion
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB21652	Ferricytochrome c	MetaboCard
ECMDB23135	Ferrocytochrome c	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB21245	Menaquinol 8	MetaboCard
ECMDB02878	Nitrate	MetaboCard
ECMDB02786	Nitrite	MetaboCard
ECMDB23788	Selenate	MetaboCard
ECMDB21355	Selenite	MetaboCard
ECMDB01060	Ubiquinol-8	MetaboCard
ECMDB21296	Ubiquinone-8	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
binding
protein binding
unfolded protein binding
Process
cellular component assembly
cellular component organization
cellular component organization or biogenesis
cellular protein complex assembly
chaperone-mediated protein complex assembly
macromolecular complex assembly
protein complex assembly

Gene Properties

Blattner:

b1466

Gene Orientation

Counterclockwise

Centisome Percentage:

33.08

Left Sequence End

1534638

Right Sequence End

1535333

Gene Sequence:

>696 bp
ATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGA
CTTGAACCGCAAGCAGAAGATGCGCCGCTTAACAACTATGCTAACGAGCGGCTTACCCAA
CTTAACCAACAGCAGCGCTGTGACGCGTTTATTCAGTTTGCCAGCGATGTTATGTTGACG
CGCTGTGAGCTGACCAGCGAGGCGAAGGCGGCAGAAGCTGCAATACGTCTGTGTAAAGAG
CTGGGAGATCAGTCGGTCGTGATTAGCGGTGACACGAGGCTGGAGGAATTGGGGATTAGC
GAACGTTTGCAGCAGGAATGCAATGCCGTTGTTTGGGATCCGGCGAAAGGTGCCGAGAAT
ATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAA
TCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCG
GAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCA
GAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTCCTGCATTAACATCATT
AGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCATGGCCCG
GTGAAAGCGGTGTATCTGATTATTGAGGATTGTTGA

Protein Properties

Pfam Domain Function:

Nitrate_red_del (PF02613 )

Protein Residues:

231

Protein Molecular Weight:

26160

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Probable nitrate reductase molybdenum cofactor assembly chaperone NarW
MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLLNAPLLDKQAEWCEVFD
RGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRELPDYLPLYLEYLSVLPDD
QAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGSSLSSDSVTKQVNSEERDDTR
QALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDVAPQYVDISAGGGK

References

External Links:

Resource	Link
Uniprot ID:	P19317
Uniprot Name:	NARW_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674452
Ecogene ID:	EG10645
Ecocyc:	EG10645
ColiBase:	b1466
Kegg Gene:	b1466
EchoBASE ID:	EB0639
CCDB:	NARW_ECOLI
BacMap:	16129425

General Reference:

Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
Blasco, F., Iobbi, C., Ratouchniak, J., Bonnefoy, V., Chippaux, M. (1990). "Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon." Mol Gen Genet 222:104-111. Pubmed: 2233673
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553