Identification
Name:Probable nitrate reductase molybdenum cofactor assembly chaperone NarW
Synonyms:
  • Redox enzyme maturation protein NarW
Gene Name:narW
Enzyme Class:Not Available
Biological Properties
General Function:Involved in unfolded protein binding
Specific Function:Chaperone required for proper molybdenum cofactor insertion and final assembly of the membrane-bound respiratory nitrate reductase 2
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Acceptor+1.0Thumb+1.0Acceptor1.0Thumb+1.0Reduced acceptor+1.0Reduced acceptor
1.0Nitrite + 1.0Acceptor + 1.0Water + 1.0Acceptor ↔ 1.0Nitrate + 1.0Reduced acceptor + 1.0Reduced acceptor
ReactionCard
2.0Ferricytochrome c+1.0Thumb+1.0Thumb1.0Thumb+2.0Ferrocytochrome c+2.0Thumb
2.0Ferricytochrome c + 1.0Nitrite + 1.0Water ↔ 1.0Nitrate + 2.0Ferrocytochrome c + 2.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.0Nitrate+1.0cytochrome c nitrite reductase+1.0Thumb1.0Nitrite+1.0cytochrome c nitrite reductase+1.0Thumb+1.0Thumb
1.0Nitrate + 1.0cytochrome c nitrite reductase + 1.0Nitrate ↔ 1.0Nitrite + 1.0cytochrome c nitrite reductase + 1.0Water + 1.0Nitrite
ReactionCard
1.0Thumb+2.0Thumb+2.0Electron1.0Thumb+1.0Thumb
1.0Selenate + 2.0Hydrogen ion + 2.0Electron → 1.0Selenite + 1.0Water
ReactionCard
1.0Thumb+2.0Thumb+1.0a menaquinol1.0Thumb+1.0Thumb+1.0a menaquinone
1.0Nitrate + 2.0Hydrogen ion + 1.0a menaquinol → 1.0Nitrite + 1.0Water + 1.0a menaquinone
ReactionCard
Complex Reactions:
2.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+2.0Thumb
2.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Menaquinone 8+1.0Thumb+2.0Thumb
2.0Hydrogen ion + 1.0Menaquinol 8 + 1.0Nitrate → 1.0Water + 1.0Menaquinone 8 + 1.0Nitrite + 2.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21652Ferricytochrome cMetaboCard
ECMDB23135Ferrocytochrome cMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB02878NitrateMetaboCard
ECMDB02786NitriteMetaboCard
ECMDB23788SelenateMetaboCard
ECMDB21355SeleniteMetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
binding
protein binding
unfolded protein binding
Process
cellular component assembly
cellular component organization
cellular component organization or biogenesis
cellular protein complex assembly
chaperone-mediated protein complex assembly
macromolecular complex assembly
protein complex assembly
Gene Properties
Blattner:b1466
Gene OrientationCounterclockwise
Centisome Percentage:33.08
Left Sequence End1534638
Right Sequence End1535333
Gene Sequence:
>696 bp
ATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGA
CTTGAACCGCAAGCAGAAGATGCGCCGCTTAACAACTATGCTAACGAGCGGCTTACCCAA
CTTAACCAACAGCAGCGCTGTGACGCGTTTATTCAGTTTGCCAGCGATGTTATGTTGACG
CGCTGTGAGCTGACCAGCGAGGCGAAGGCGGCAGAAGCTGCAATACGTCTGTGTAAAGAG
CTGGGAGATCAGTCGGTCGTGATTAGCGGTGACACGAGGCTGGAGGAATTGGGGATTAGC
GAACGTTTGCAGCAGGAATGCAATGCCGTTGTTTGGGATCCGGCGAAAGGTGCCGAGAAT
ATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAA
TCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCG
GAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCA
GAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTCCTGCATTAACATCATT
AGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCATGGCCCG
GTGAAAGCGGTGTATCTGATTATTGAGGATTGTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:231
Protein Molecular Weight:26160
Protein Theoretical pI:4
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Probable nitrate reductase molybdenum cofactor assembly chaperone NarW
MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLLNAPLLDKQAEWCEVFD
RGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRELPDYLPLYLEYLSVLPDD
QAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGSSLSSDSVTKQVNSEERDDTR
QALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDVAPQYVDISAGGGK
References
External Links:
ResourceLink
Uniprot ID:P19317
Uniprot Name:NARW_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674452
Ecogene ID:EG10645
Ecocyc:EG10645
ColiBase:b1466
Kegg Gene:b1466
EchoBASE ID:EB0639
CCDB:NARW_ECOLI
BacMap:16129425
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blasco, F., Iobbi, C., Ratouchniak, J., Bonnefoy, V., Chippaux, M. (1990). "Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon." Mol Gen Genet 222:104-111. Pubmed: 2233673
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553